Long Noncoding RNA PSMB8-AS1 Mediates the Tobacco-Carcinogen-Induced Transformation of a Human Bronchial Epithelial Cell Line by Regulating Cell Cycle.

Lung cancer is the main cause of cancer deaths around the world. Nitrosamine 4-(methyl nitrosamine)-1-(3-pyridyl)-1-butanone (NNK) is a tobacco-specific carcinogen of lung cancer. Abundant evidence implicates long noncoding RNAs (lncRNAs) in tumorigenesis.

[Effects of high magneto-gravitational environment on calcium/calmodulin signal of MG63 osteoblast-like cells].

Aim: To investigate the effects of high magneto-gravitational environment on Ca(2+);]/calmodulin (CaM) signal of MG63 osteoblast-like cells.

Methods: A special designed large gradient high magnetic field could produce three different high magneto-gravitational environments including μg (12 T), 1 g (16 T) and 2 g (12 T). The effects of high magneto-gravitational environments on intracellular free Ca(2+);] concentration ([Ca(2+);](i);) and protein expression including calmodulin (CaM), myosin light chain kinases (MLCK) and phosphorylated Ca(2+);]/CaM dependent protein kinase II(pCaMKII) were measured by Fluo-3/AM or Western blot, respectively.

[Roles of autocrine soluble fibronectin on the maintenance of cell shape of osteosarcoma MG-63 cells].

Aim: To investigate the roles of autocrine soluble FN of MG-63 cells in cell shape maintaining.

Methods: Human osteosarcoma MG-63 cells (1 × 10(4); cells/cm(2);) were routinely cultured in MEM medium+10% fetal bovine serum (FBS) for 24 h and then the medium was replaced by fresh medium(the group without autocrine soluble FN), conditional medium of collected after 24 h of MG-63 cells (the group containing autocrine soluble FN ) and fresh medium with 20 microg/mL FN( the group with plasma FN) after 24 h, and the cell morphology was observed using phase contrast microscope; The concentrations of soluble FN in fresh and conditional medium were detected; Microfilament and insoluble FN changes of MG-63 cells were investigated.

Results: The concentration of soluble FN in conditional medium is much higher than that in the fresh medium (P<0.