Publications by authors named "Sheng-Nan Huang"

Background: Although quercetin exhibits promising anti-tumor properties, its clinical application is limited due to inherent defects and a lack of tumor targeting.

Objectives: This study aimed to prepare and characterize active targeting folate-chitosan modified quercetin liposomes (FA-CS-QUE-Lip), and its antitumor activity and was also studied.

Materials And Methods: Box-Behnken Design (BBD) response surface method was used to select the optimal formulation of quercetin liposomes (QUE-LP).

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  • Human cytomegalovirus (HCMV) is linked to congenital birth defects due to its negative impact on neuronal migration, specifically through the downregulation of connexin 43 (Cx43).
  • The viral protein IE1 has been identified as the key player in reducing Cx43 levels by binding to its C terminus and triggering its degradation via the ubiquitin-proteasome pathway.
  • Experimental evidence from mouse models shows that the introduction of IE1 leads to cortical atrophy and migration issues, highlighting a potential target for intervention in HCMV-related neural maldevelopment.
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  • Congenital human cytomegalovirus (HCMV) infection disrupts fetal brain development by upregulating suppressor of cytokine signaling 3 (SOCS3) in neural progenitor cells (NPCs).
  • The viral protein pUL97 was identified as a key factor that prolongs SOCS3 expression by interacting with the transcription factor regulatory factor X 7 (RFX7).
  • Increased SOCS3 levels lead to impaired proliferation and migration of NPCs, revealing a new mechanism by which HCMV affects fetal brain development and cytokine signaling.
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The lateral hypothalamus (LH) is an important brain region mediating sleep-wake behavior. Recent evidence has shown that astrocytes in central nervous system modulate the activity of adjacent neurons and participate in several physiological functions. However, the role of LH astrocytes in sleep-wake regulation remains unclear.

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The dorsal raphe nucleus (DRN) has previously been proved to be involved in the regulation of the sleep-wake behavior. DRN contains several neuron types, such as 5-HTergic and GABAergic neurons. GABAergic neurons, which are the second largest cell subtype in the DRN, participate in a variety of neurophysiological functions.

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During the long coevolution of human cytomegalovirus (HCMV) and humans, the host has formed a defense system of multiple layers to eradicate the invader, and the virus has developed various strategies to evade host surveillance programs. The intrinsic immunity primarily orchestrated by promyelocytic leukemia (PML) nuclear bodies (PML-NBs) represents the first line of defense against HCMV infection. Here, we demonstrate that microrchidia family CW-type zinc finger 3 (MORC3), a PML-NBs component, is a restriction factor targeting HCMV infection.

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Aflatoxin M1 (AFM1) and ochratoxin A (OTA), which are occasionally detected in milk and commercial baby foods, could easily enter and reach the gastrointestinal tract, posing impairment to the first line of defense and causing dysfunction of the tissue. The objective of this study was to investigate the immunostimulatory roles of individual and combined AFM1 and OTA on the immature intestine. Thus, we used ELISA assays to evaluate the generation of cytokines from ex vivo CD-1 fetal mouse jejunum induced by AFM1 and OTA and explored the related regulatory pathways and pivot genes using RNA-seq analysis.

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Defensive behavior, a group of responses that evolved due to threatening stimuli, is crucial for animal survival in the natural environment. For defensive measures to be timely and successful, a high arousal state and immediate sleep-to-wakefulness transition are required. Recently, the glutamatergic basal forebrain (BF) has been implicated in sleep-wake regulation; however, the associated physiological functions and underlying neural circuits remain unknown.

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  • Human cytomegalovirus (HCMV) has a complex genome and relies on various cellular factors for replication, particularly emphasizing the role of the host protein WDR11 in the formation of a specialized compartment for virion assembly.
  • WDR11 is upregulated during HCMV infection and is crucial for the reorganization of cellular membranes, specifically aiding in the creation of the virion assembly compartment (vAC) necessary for the maturation of virions.
  • Disruption of WDR11 function impairs HCMV replication by affecting the Golgi apparatus' remodeling, vAC formation, and ultimately the production of infectious virions, highlighting its essential role in viral life cycle stages.
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Congenital cytomegalovirus (cCMV) infection is the leading infectious cause of neurodevelopmental disorders. However, the neuropathogenesis remains largely elusive due to a lack of informative animal models. In this study, we developed a congenital murine CMV (cMCMV) infection mouse model with high survival rate and long survival period that allowed long-term follow-up study of neurodevelopmental disorders.

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  • Human cytomegalovirus (HCMV) can establish a persistent infection, which poses risks of severe illness, especially in newborns and people with weakened immune systems.
  • In a study involving T98G cells, researchers identified 168 proteins that changed during HCMV latency and reactivation, highlighting the molecular mechanisms involved.
  • The study also found that proteins like ApoE and the PI3K pathway significantly influence HCMV infection status, with ApoE promoting latency and blocking the PI3K pathway triggering reactivation.
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Hearing loss is one of the most prevalent sensory disabilities worldwide with huge social and economic burdens. The leading cause of sensorineural hearing loss (SNHL) in children is congenital cytomegalovirus (CMV) infection. Though the implementation of universal screening and early intervention such as antiviral or anti-inflammatory ameliorate the severity of CMV-associated diseases, direct and targeted therapeutics is still seriously lacking.

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We previously reported that human cytomegalovirus (HCMV) utilizes the cellular protein WD repeat-containing protein 5 (WDR5) to facilitate capsid nuclear egress. Here, we further show that HCMV infection results in WDR5 localization in a juxtanuclear region, and that its localization to this cellular site is associated with viral replication and late viral gene expression. Furthermore, WDR5 accumulated in the virion assembly compartment (vAC) and co-localized with vAC markers of gamma-tubulin (γ-tubulin), early endosomes, and viral vAC marker proteins pp65, pp28, and glycoprotein B (gB).

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Predatory hunting is an important approach for animals to obtain valuable nutrition and energy, which critically depends on heightened arousal. Yet the neural substrates underlying predatory hunting remain largely undefined. Here, we report that basal forebrain (BF) GABAergic neurons play an important role in regulating predatory hunting.

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The glutamatergic lateral hypothalamus (LH) has been implicated in a variety of behaviors, such as evasion and feeding, while its role in defensive behaviors and relevant neurocircuits remains unclear. Here, we demonstrated that the glutamatergic LH is a critical structure regulating defensive behaviors. Trimethylthiazole (TMT), the odor of mice predator, significantly increased c-Fos expression in the LH.

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Background: Visceral pain is one of the most common types of pain and particularly in the abdomen is associated with gastrointestinal diseases. Bulleyaconitine A (BAA), isolated from , is prescribed in China to treat chronic pain. The present study is aimed at evaluating the mechanisms underlying BAA visceral antinociception.

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The obesity epidemic is a global problem and a great challenge for public health. Overconsumption of food, especially palatable food, is the leading cause of obesity. The precise neural circuits underlying food overconsumption remain unclear and require further characterization.

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Visceral pain is one of the leading causes for abdominal pain in gastroenterological diseases and is still hard to treat effectively. Bulleyaconitine A (BAA) is an aconitine analog and has been used for the treatment of pain. Our previous work suggested that BAA exerted analgesic effects on neuropathic pain through stimulating the expression of dynorphin A in spinal microglia.

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  • The study aimed to assess how lactoferrin, α-lactalbumin, and β-lactoglobulin can protect against cerebral ischemia-reperfusion (I/R) injuries using both cell cultures and mouse models.
  • The experiment measured levels of toll-like receptor 4 (TLR4) and other inflammatory markers, finding that these proteins protect neurons by modulating the TLR4 pathway.
  • Among the three proteins, β-lactoglobulin demonstrated the highest protective activity, suggesting that TLR4 could be a new target for preventing brain injuries during strokes.
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  • MicroRNA (miRNA) therapy faces challenges in cancer treatment due to its degradation and instability, making effective delivery to target cells difficult.
  • Researchers developed a novel delivery system using gold nanocages modified with specific materials (AuNCs/PEI/miRNA/HA) to facilitate targeted intracellular delivery of miRNA through receptor-mediated endocytosis.
  • Results showed that the delivery system effectively targeted hepatocellular carcinoma (HCC) cells, enhancing antitumor effects through a combination of gene therapy and photothermal therapy, and indicating its potential for HCC treatment.
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Novel bioactive heterocycles containing a 3,4,5-trimethoxyphenyl fragment as antiproliferative agents by targeting tubulin were synthesized and their preliminary structure activity relationships (SARs) were explored. Among all these chemical agents, 2-(Benzo[d]oxazol-2-ylthio)-N-(4-methoxybenzyl)-N-(3,4,5-trimethoxyphenyl)acetamide (4d) exhibited the potent antiproliferative activity against MGC-803 cells with an IC value of 0.45 μM by induction of G2/M pahse arrest and cell apoptosis.

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A previous report showed that both () genes were significantly upregulated in kiwifruit after waterlogging treatment using Illumina sequencing technology, and that the kiwifruit gene was required during waterlogging, but might not be required during other environmental stresses. Here, the function of another gene, named , was analyzed. The expression of the gene was determined using qRT-PCR, and the results showed that the expression levels of in the reproductive organs were much higher than those in the nutritive organs.

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  • Congenital human cytomegalovirus (HCMV) infection is a major cause of neurological disabilities in children, with unclear mechanisms behind these effects.
  • The study highlights that HCMV infection influences the Notch signaling pathway by reducing levels of Hes1, a key regulator for neural progenitor cells, potentially impacting fetal brain development.
  • The HCMV protein IE1 promotes Hes1 degradation through ubiquitination and is identified as a novel potential E3 ubiquitin ligase, shedding light on the link between HCMV infection and neurodevelopmental disorders.
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  • Human cytomegalovirus (HCMV) infection is a major cause of birth defects, particularly affecting brain development.
  • The study highlights the importance of Hes1, a key regulator in the Notch signaling pathway, which shows oscillatory expression in human neural progenitor cells (NPCs), with a cycle of about 1.5 hours.
  • HCMV infection disrupts this Hes1 rhythm and lowers its expression, leading to negative impacts on NPC proliferation and differentiation, potentially contributing to neurodevelopmental disorders linked to congenital HCMV infections.
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Ethanolic fermentation is classically associated with waterlogging tolerance when plant cells switch from respiration to anaerobic fermentation. Pyruvate decarboxylase (PDC), which catalyzes the first step in this pathway, is thought to be the main regulatory enzyme. Here, we cloned a full-length PDC cDNA sequence from kiwifruit, named AdPDC1.

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