Publications by authors named "Sheng Kai Pong"
In mammalian cells, small non-coding RNAs (sncRNAs) negatively regulate gene expression in a pathway known as RNA interference (RNAi). RNAi can be categorized into post-transcriptional gene silencing (PTGS), which involves the cleavage of target messenger RNA (mRNA) or inhibition of translation in the cytoplasm, and transcriptional gene silencing (TGS), which is mediated by the establishment of repressive epigenetic marks at target loci. Transfer RNAs (tRNAs), which are essential for translation, can be processed into small ncRNAs, termed tRNA-derived small RNAs (tsRNAs).
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- Eukaryotic genomes produce long intergenic non-coding RNAs (lincRNAs) that may play a role in gene regulation, but their overall contribution to the connection between genetics and observable traits (phenotype) is not well understood.
- Researchers used CRISPR/Cas9 to delete 141 lincRNA genes and analyzed their effects alongside 238 coding-gene mutants by testing growth and viability under various conditions, uncovering phenotypes for nearly 60% of the lincRNA deletions.
- Additional experiments involving overexpression of 113 lincRNAs revealed that 90.3% of these strains showed altered growth, suggesting that lincRNAs have specific cellular functions that vary with environmental conditions, laying the
Centrioles are microtubule-based organelles crucial for cell division, sensing and motility. In Caenorhabditis elegans, the onset of centriole formation requires notably the proteins SAS-5 and SAS-6, which have functional equivalents across eukaryotic evolution. Whereas the molecular architecture of SAS-6 and its role in initiating centriole formation are well understood, the mechanisms by which SAS-5 and its relatives function is unclear.
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