Population-level amplification of gene regulation by programmable gene transfer.

Engineering cells to sense and respond to environmental cues often focuses on maximizing gene regulation at the single-cell level. Inspired by population-level control mechanisms like the immune response, we demonstrate dynamic control and amplification of gene regulation in bacterial populations using programmable plasmid-mediated gene transfer. By regulating plasmid loss rate, transfer rate and fitness effects via Cas9 endonuclease, F conjugation machinery and antibiotic selection, we modulate the fraction of plasmid-carrying cells, serving as an amplification factor for single-cell-level regulation.

Cocoyam powder extracted from as a novel plant-based bioflocculant for industrial wastewater treatment: Flocculation performance and mechanism.

In the current investigation, the comparative study of cocoyam bioflocculant (CYBF) and chemical flocculant for the removal of heavy metals, COD, BOD, TDS, TSS, sulphate and nitrate from tannery effluent, and dyes from synthetic dye wastewater were examined. Different analytical techniques, including Fourier transforms infrared (FTIR), X-ray powder diffraction (XRD), Scanning electron microscopy (SEM), and energy dispersive spectroscopy (EDX), were used to characterise the extracted bioflocculant. FTIR spectral measurement of the bioflocculant demonstrated the presence of hydroxyl, carboxyl, and amino groups.

Evaluation of the Effects of Caesalpinia crista on Letrozole-Induced Models of Polycystic Ovarian Syndrome.

Background Polycystic ovarian syndrome (PCOS) is the most common endocrine disorder in women of reproductive age, affecting reproductive, endocrine, and metabolic functions. This study was designed to validate the claims in Ayurveda regarding the efficacy of (Latakaranj) to treat PCOS. Its seeds are uterine stimulants and ovulation inducers and improve menstrual cycle irregularities.

Zwitterion to normal formation of L-Alanine in water solvation as an ultrasonic impact from their Gibbs energy barrier: Experiment with different molarities and DFT simulation for few basis sets.

l-Alanine has Physiological significance, and their molecular interaction is subjected to the Gibbs energy barrier. The present study has reported the Gibb's energy barrier of l-Alanine (l-Ala) from 20.095 to 20.

Biologia Futura: treatment of wastewater and water using tannin-based coagulants.

Industrialization and urbanization are mainly responsible for environmental pollution generating enormous amount of wastewater which needs to be treated. Wastewaters from various sources are toxic to humans and livestock, as well as posing environmental risks. Various treatment approaches have been used for the elimination of contaminants from water and wastewater.

An improved primary wastewater treatment system for a slaughterhouse industry: a full-scale experience.

The effluent streams from individual slaughtering operations were segregated based on the degree of similarity and were treated separately. The wastewater from lairage and paunch sections was dominant in suspended solids (SS: 6,000-25,000 mg/L) and was separated using a hydrasieve (500 μm) and externally fed rotary drum filter (EFRDF, 200 μm), respectively. The SS removal efficiency of the hydrasieve and EFRDF was 75% and 55%, respectively, and remaining solids were removed through a primary clarifier.

Intrauterine bony fragments - An unexpected finding in the hysterectomy specimen.

Intrauterine bony fragments (IUBF) are an unusual finding in hysterectomy specimen received in a histopathology laboratory. Females harboring IUBF may present non-specific symptoms like vaginal bleeding, leukorrhea, chronic pelvic pain, and secondary infertility. Herein we report the case of a 35-year-old female who presented vaginal discharge and bleeding for two years, since when she had an abortion.

Anaerobic treatment of slaughterhouse wastewater: a review.

This article presents a review of anaerobic treatment technologies to treat slaughterhouse wastewater including its advantages and disadvantages. Physico-chemical characteristics and biochemical methane potential (BMP) of slaughterhouse wastewater are addressed. Various anaerobic treatment technologies are presented with the related operating parameters, viz.

Treatment of low strength wastewater using compact submerged aerobic fixed film (SAFF) reactor filled with high specific surface area synthetic media.

Studies on laboratory-scale submerged aerobic fixed film reactor (SAFF) packed with synthetic media having specific surface area of 165 m/m with a void volume of 89% were carried out to assess its performance under various organic loading rates (OLR) and hydraulic retention times (HRT). Synthetic wastewater having chemical oxygen demand (COD) and biochemical oxygen demand (BOD) of 400 ± 10% and 210 ± 10% mg/L respectively was fed and the reactor was subjected to OLRs ranging from 0.37 to 1.

An audit of studies registered retrospectively with the Clinical Trials Registry of India: A one year analysis.

Background: The Clinical Trials Registry of India (CTRI) was launched in July 2007 and will enter its tenth year in 2017. While its mission is to encourage prospective trial registration, CTRI does permit retrospective trial registration. Against this backdrop, the present audit was carried out with the primary objective of assessing the nature and extent of trials retrospectively registered with CTRI.

Management of fenestration using bone allograft in conjunction with platelet-rich fibrin.

Fenestration and dehiscence are said to be anatomical variations of cortical bone and not true pathological entities. They represent window-like defects covered by periosteum and overlying gingiva with or without the intact marginal bone. The etiology of such defects is still unclear, though many hypotheses such as occlusal traumatism, trauma, and variation in root bone angulation have been put forward.

Effect of supplementation of recombinant Regucalcin in extender on cryopreservation of spermatozoa of water buffalo (Bubalus bubalis).

Elevated intracellular calcium concentration and oxidative damage are two major factors contributing to the poor fertility of cryopreserved spermatozoa. Regucalcin (RGN), also known as Senescence marker protein-30 (SMP-30), is a calcium-binding protein with multiple roles that include calcium homeostasis, anti-oxidative, anti-apoptosis, and anti-proliferation. In Drosophila, RGN is reportedly a putative cold-tolerance gene and a cytoprotective role for RGN against intracellular calcium elevation and oxidative stress was reported in P19 cell lines.

Detection and localization of regucalcin in spermatozoa of water buffalo (Bubalus bubalis): A calcium-regulating multifunctional protein.

Regucalcin (RGN) is a calcium-regulating, anti-apoptotic, antioxidative and antiproliferative multifunctional protein predominantly seen in liver and kidney. All these functions are very crucial during spermatogenesis and sperm maturation process until fertilization of the ovum. Although many studies have reported the wide distribution of regucalcin in the male reproductive tract of the rat, human and bovine, its presence in spermatozoa is yet to be demonstrated wherein calcium has a pivotal role in the transport, capacitation, acrosomal reaction and further fusion with ova.

Calcium Binding Ability of Recombinant Buffalo Regucalcin: A Study Using Circular Dichroism Spectroscopy.

Regucalcin is a calcium regulating multifunctional protein reported to have many important functions like calcium homeostasis, anti-oxidative, anti-apoptotic and anti-cancerous functions. Although it is demonstrated as a calcium regulating protein, the calcium binding ability of regucalcin is still a controversy. The main reason for the controversy is that it lacks a typical EF hand motif which is common to most of the calcium binding proteins.

Regucalcin is widely distributed in the male reproductive tract and exerts a suppressive effect on in vitro sperm capacitation in the water buffalo (Bubalus bubalis).

Regucalcin is a multi-functional, calcium-binding protein with roles in calcium homeostasis, apoptosis, cell proliferation, and free radical neutralization. Regucalcin is broadly expressed in the male reproductive organs of rat and bovine; here, we report its expression in the reproductive tract of male buffalo-especially in testis, epididymis, seminal vesicle, prostate, and bulbourethral gland of buffalo-as analyzed by real-time PCR, Western blot, and immunolocalization. Regucalcin degradation in seminal plasma, despite its high abundance in vesicular fluid, was demonstrated using recombinant regucalcin co-incubated with buffalo seminal plasma.

Purification of Regucalcin from the Seminal Vesicular Fluid: A Calcium Binding Multi-Functional Protein.

Regucalcin is a multi-functional protein having roles in calcium homeostasis as well as in anti-apoptotic, anti-prolific and anti-oxidative functions. Recently, it has been reported from the male reproductive tract, but its role in male reproduction needs further investigation; for which the native regucalcin of reproductive origin will be more appropriate. The gel exclusion chromatography followed by diethyl aminoethane cellulose chromatography and two-dimentional cellulose acetate membrane electrophoresis used for its purification are time consuming and less specific.