Mapping the carbon footprint of milk production from cattle: A systematic review.

Recently, many studies have assessed the carbon footprint of bovine milk production. However, due to the complexity of life cycle assessment, most studies have analyzed research farms or "representative" farms, which do not capture farm variability. Furthermore, the lack of consistency in most studies means that we can seldom compare the footprint between different countries.

IGF1 does not overcome sexual dimorphism of body and muscle size in Mstn-/- mice.

Insulin-like growth factor-1 (IGF1) is crucial for regulating post-natal growth and, along with myostatin (MSTN), regulates muscle size. Here, we sought to clarify the roles of these two genes in regulating sexually dimorphic growth of body and muscle mass. In the first study, we established that Igf1 mRNA was increased to a greater extent and Igf1 receptor mRNA increased earlier in male, than in female, gastrocnemius muscles during the rapid phase of growth (from 2 to 6 weeks) were unchanged, thereafter, to 32 weeks of age in WT mice (P < 0.

Eutrophication and climate change impacts of a case study of New Zealand beef to the European market.

Objective: Beef production in the Lake Taupō region of New Zealand (NZ) is regulated for nitrogen (N) leaching. The objectives of this study were to 1) evaluate the implications of nitrogen emission limitations on eutrophication and climate change impacts of NZ beef through its life cycle to a European market and uniquely link it to 2) estimation of the reduction in these impacts that can be funded by the consumer's willingness to pay (WTP) a premium for a low environmental-impact product.

Method: The cradle-to-market Life Cycle Assessment (LCA) of NZ beef on the European market included beef production on farms, meat processing, packaging and transport stages.

Water scarcity footprint of dairy milk production in New Zealand - A comparison of methods and spatio-temporal resolution.

Water scarcity footprinting now has a consensual life cycle impact assessment indicator recommended by the UNEP/SETAC Life Cycle Initiative called AWaRe. It was used in this study to calculate the water scarcity footprint of New Zealand (NZ) milk produced in two contrasting regions; "non-irrigated moderate rainfall" (Waikato) and "irrigated low rainfall" (Canterbury). Two different spatial and temporal resolutions for the inventory flows and characterisation factors (CFs) were tested and compared: country and annual vs.

IGF1 stimulates greater muscle hypertrophy in the absence of myostatin in male mice.

Insulin-like growth factors (IGFs) and myostatin have opposing roles in regulating the growth and size of skeletal muscle, with IGF1 stimulating, and myostatin inhibiting, growth. However, it remains unclear whether these proteins have mutually dependent, or independent, roles. To clarify this issue, we crossed myostatin null () mice with mice overexpressing in skeletal muscle () to generate six genotypes of male mice; wild type ( ), , , , and Overexpression of increased the mass of mixed fibre type muscles (e.

Translational signalling, atrogenic and myogenic gene expression during unloading and reloading of skeletal muscle in myostatin-deficient mice.

Skeletal muscles of myostatin null (Mstn(-/-)) mice are more susceptible to atrophy during hind limb suspension (HS) than are muscles of wild-type mice. Here we sought to elucidate the mechanism for this susceptibility and to determine if Mstn(-/-) mice can regain muscle mass after HS. Male Mstn(-/-) and wild-type mice were subjected to 0, 2 or 7 days of HS or 7 days of HS followed by 1, 3 or 7 days of reloading (n = 6 per group).

Identification and expression of a novel transcript of the growth and differentiation factor-11 gene.

The growth and differentiation factor-11 (GDF-11) gene is thought to code for a single protein that plays a crucial role in regulating the development of multiple tissues. In this study, we aimed to investigate if the GDF-11 gene has another transcript and, if so, to characterise this transcript and determine its tissue-specific and developmental expression. We have identified a novel transcript of GDF-11 in mouse muscle, which contains the 3' region of intron 1, exon 2, exon 3 and 3'UTR, and has two transcription initiation sites and a single termination site.

Discovery of a mammalian splice variant of myostatin that stimulates myogenesis.

Myostatin plays a fundamental role in regulating the size of skeletal muscles. To date, only a single myostatin gene and no splice variants have been identified in mammals. Here we describe the splicing of a cryptic intron that removes the coding sequence for the receptor binding moiety of sheep myostatin.

The decrease in mature myostatin protein in male skeletal muscle is developmentally regulated by growth hormone.

Myostatin inhibits myogenesis and there is reduced abundance of the mature protein in skeletal muscles of adult male compared with female mice. This reduction probably occurs after translation, which suggests that it is a regulated mechanism to reduce the availability of myostatin in males. Reduced myostatin may, thereby, contribute to the development of sexually dimorphic growth of skeletal muscle.

Effect of botulinum toxin A-induced paralysis and exercise training on mechanosensing and signalling gene expression in juvenile rat gastrocnemius muscle.

Intramuscular injections of the paralytic botulinum neurotoxin A (Btx) and physical exercise are used in the treatment of chronic spasticity in children with cerebral palsy. We tested whether Btx-induced paralysis and/or exercise training would have differential effects on the expression of mechanosensing and signalling genes implicated in the adaptive remodelling of skeletal muscle. Juvenile (29-day-old) male rats were injected with Btx or saline (NoBtx) into the right gastrocnemius and housed in standard cages (NoEx) or with running wheels (Ex), for 3 weeks (n = 6 per group).