Publications by authors named "Sheila Khanh Nguyen"
The role of oxidative stress in alcoholic liver disease and cytokeratin aggresome formation is the focus of this in vitro study. HepG2 cells transduced to over express CYP2E1 (E47) and control HepG2 cells (C34) were first treated with arachidonic acid, then Fe-NAT, and finally with ethanol. In the E47 ethanol-treated cells, CYP2E1 was induced and a higher level of reactive oxygen species and carbonyl proteins were generated.
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- The study investigates the role of valosin-containing protein (VCP) in the formation of Mallory bodies (MBs) using primary cultured hepatocytes from drug-primed mice.
- Inhibiting VCP expression with gene-specific gripNA led to a significant increase (230%) in MB formation in drug-primed hepatocytes, suggesting VCP normally inhibits MB formation.
- Overexpression of VCP did not reduce MB formation, indicating that VCP may promote MB formation through its molecular chaperone activity within the ubiquitin-proteasome system.