Fowl typhoid live lyophilized vaccine applied at 3-month intervals protected layer chickens from infection and prevented cloacal shedding.

Objective: Here, we developed and tested the efficacy of a vaccination protocol based on a commercially available live attenuated serovar Gallinarum () in layer chickens.

Materials And Methods: 50 layer chickens of 16 weeks age were obtained and divided into two groups ( = 25), control and vaccinated. The vaccinated group received vaccine at 0.