Adaptive stimulation of macropinocytosis overcomes aspartate limitation in cancer cells under hypoxia.

Stress-adaptive mechanisms enable tumour cells to overcome metabolic constraints under nutrient and oxygen shortage. Aspartate is an endogenous metabolic limitation under hypoxic conditions, but the nature of the adaptive mechanisms that contribute to aspartate availability and hypoxic tumour growth are poorly understood. Here we identify GOT2-catalysed mitochondrial aspartate synthesis as an essential metabolic dependency for the proliferation of pancreatic tumour cells under hypoxic culture conditions.

Targeting the α4-α5 interface of RAS results in multiple levels of inhibition.

Generation of RAS-targeted therapeutics has long been considered a "holy grail" in cancer research. However, a lack of binding pockets on the surface of RAS and its picomolar affinity for guanine nucleotides have made isolation of inhibitors particularly challenging. We recently described a monobody, termed NS1, that blocks RAS signaling and oncogenic transformation.

Clustering of Toxoplasma gondii Infections Within Families of Congenitally Infected Infants.

Background: Family clusters and epidemics of toxoplasmosis in North, Central, and South America led us to determine whether fathers of congenitally infected infants in the National Collaborative Chicago-Based Congenital Toxoplasmosis Study (NCCCTS) have a high incidence of Toxoplasma gondii infection.

Methods: We analyzed serum samples collected from NCCCTS families between 1981 and 2013. Paternal serum samples were tested for T.

Use of complementaryand alternative therapies among rural TB patients in Nalgonda, Andhra Pradesh: a qualitative study.

Background: A considerable number of studies have been conducted on health seeking behaviour of TB patients in different parts of the world. However, these studies largely ignored the prevalence and use of complementary and alternative medical practices (CAM) among TB patients. This present study was prompted by the recognition that, an understanding of different factors influencing CAM use in a medically pluralistic setting has important implications for providing patient-centred comprehensive care, and improving the quality of life for TB patients.

Psychosocial trauma of diagnosis: a qualitative study on rural TB patients' experiences in Nalgonda district, Andhra Pradesh.

Background: The current tuberculosis (TB) control strategy in India largely ignores psychosocial needs of the patients. The present study was prompted by the recognition that, if TB treatment is to be culturally sensitive and effective, the psychosocial problems and issues need to be recognized and addressed.

Aims: The main aim of this study was to explore psychosocial problems and issues among rural patients being diagnosed with TB.