Cold dispase digestion of murine lungs improves recovery and culture of airway epithelial cells.

Airway epithelial cells (AECs) play a key role in maintaining lung homeostasis, epithelium regeneration and the initiation of pulmonary immune responses. To isolate and study murine AECs investigators have classically used short and hot (1h 37°C) digestion protocols. Here, we present a workflow for efficient AECs isolation and culture, utilizing long and cold (20h 4°C) dispase II digestion of murine lungs.

The complex dynamics of the 2023 Kahramanmaraş, Turkey, 7.8-7.7 earthquake doublet.

The destructive 2023 moment magnitude () 7.8-7.7 earthquake doublet ruptured multiple segments of the East Anatolian Fault system in Turkey.

Immunohistochemical analysis of expression of VEGFR2, KIT, PDGFR-β, and CDK4 in canine urothelial carcinoma.

Urothelial carcinomas (UCs), also known as transitional cell carcinomas, are the most common canine urinary tract neoplasms. Tyrosine kinases (TKs) are enzymes that tightly regulate cell growth and differentiation through phosphorylation. Receptor TK (RTK) inhibitors are currently used to treat UCs.

A Scoping Review of the Global Distribution of Causes and Syndromes Associated with Mid- to Late-Term Pregnancy Loss in Horses between 1960 and 2020.

Equine pregnancy loss is frustrating and costly for horse breeders. The reproductive efficiency of mares has significant implications for a breeding operation's economic success, and widespread losses can have a trickle-down effect on those communities that rely on equine breeding operations. Understanding the causes and risks of equine pregnancy loss is essential for developing prevention and management strategies to reduce the occurrence and impact on the horse breeding industry.

Oceanic plateau of the Hawaiian mantle plume head subducted to the uppermost lower mantle.

The Hawaiian-Emperor seamount chain that includes the Hawaiian volcanoes was created by the Hawaiian mantle plume. Although the mantle plume hypothesis predicts an oceanic plateau produced by massive decompression melting during the initiation stage of the Hawaiian hot spot, the fate of this plateau is unclear. We discovered a megameter-scale portion of thickened oceanic crust in the uppermost lower mantle west of the Sea of Okhotsk by stacking seismic waveforms of precursors.

Hydroureteronephrosis and fetal mummification secondary to uterine torsion in a Merino ewe.

A 6 year old pluriparous Merino ewe was presented for investigation of a large intra-abdominal mass. Post-mortem examination revealed a 360° clockwise uterine torsion was present with a mummifying fetus. The torsion involved the left ureter resulting in a severe hydroureteronephrosis.

Residual Effects of Insecticides on Deraeocoris brevis (Hemiptera: Miridae).

Deraeocoris brevis (Uhler) is a key predatory natural enemy of insects and mites in pear orchards in the United States. Insecticides used for crop protection in pear orchards and their residues can negatively affect populations of D. brevis.

Field-Aged Insecticide Residues on Chrysoperla johnsoni (Neuroptera: Chrysopidae).

We studied the direct (lethal) and indirect (sublethal) effects of field-aged insecticide residues of spinetoram, chlorantraniliprole and lambda-cyhalothrin on adults and larvae of the green lacewing, Chrysoperla johnsoni Henry, Wells and Pupedis an important generalist predator in western United States pear orchards. We applied formulated pesticides using their high label rates mixed with 935 liters/ha of water on pear trees utilizing an airblast sprayer. We collected leaves from the experiment trees at 1, 7, 14, 21 days after treatment (DAT) and exposed C.

Searching for hidden earthquakes in Southern California.

Earthquakes follow a well-known power-law size relation, with smaller events occurring much more often than larger events. Earthquake catalogs are thus dominated by small earthquakes yet are still missing a much larger number of even smaller events because of signal fidelity issues. To overcome these limitations, we applied a template-matching detection technique to the entire waveform archive of the regional seismic network in Southern California.

Multiple-Lure Surveillance Trapping for Ips Bark Beetles, Monochamus Longhorn Beetles, and Halyomorpha halys (Hemiptera: Pentatomidae).

Invasions by insects introduced via international trade continue to cause worldwide impacts. Surveillance programs using traps baited with host volatiles and pheromones can detect incursions of nonnative species. We report on two experiments executed to determine if attractants for several insect species can be combined without compromising trap catches and detection ability of target species.

Spatial Analysis of Seasonal Dynamics and Overwintering of Drosophila suzukii (Diptera: Drosophilidae) in the Okanagan-Columbia Basin, 2010-2014.

Spotted wing drosophila, Drosophila suzukii (Matsumura) (Diptera: Drosophilidae), was monitored from 2010 to 2014 in 314-828 sites located in interior fruit-growing regions of OR and WA, United States, and BC, Canada, using traps baited with apple cider vinegar or sugar-water-yeast. Seasonal population dynamics and sex ratios were summarized for berry, cherry, stone fruit, grape, non-crop host plants, non-host sites, and for conventional IPM, certified organic, backyard, and feral sites, by region and year. Overwintering was detected in all regions and years, despite winter temperatures below -17°C.

Stability of Cacopsylla pyricola (Hemiptera: Psyllidae) Populations in Pacific Northwest Pear Orchards Managed with Long-Term Mating Disruption for Cydia pomonella (Lepidoptera: Tortricidae).

This study focused on conservation biological control of pear psylla, , in the Pacific Northwest, USA. We hypothesized that insecticides applied against the primary insect pest, codling moth , negatively impact natural enemies of pear psylla, thus causing outbreaks of this secondary pest. Hence, the objective of this study was to understand how codling moth management influences the abundance of pear psylla and its natural enemy complex in pear orchards managed under long-term codling moth mating disruption programs.

Transgender and Gender Nonconforming in Emergency Departments: A Qualitative Report of Patient Experiences.

Individuals who have a transgender or gender nonconforming (TGGNC) experience belong to a marginalized segment of the U.S. population, and healthcare can be difficult for them to navigate.

Ichthyosis fetalis in Polled Hereford and Shorthorn calves.

Inherited forms of ichthyosis, or generalized scaling of the skin, have been reported in many animal species, including cattle, and are characterized by an autosomal recessive mode of inheritance. We investigated 2 calves affected with ichthyosis fetalis, a Polled Hereford and a Shorthorn. Both cases had hard white plaques on the skin consistent with excessive keratinization.

Effect of non-nutritive sugars to decrease the survivorship of spotted wing drosophila, Drosophila suzukii.

In this study, we investigated the effects of non-nutritive sugars and sugar alcohols on the survivorship of spotted wing drosophila, Drosophila suzukii, and found erythritol and erythrose as potentially insecticidal to the fly. In a dose-dependent study, erythritol and erythrose significantly reduced fly longevity, with 100% mortality with 1, 0.5, 0.

Deciphering the Routes of invasion of Drosophila suzukii by Means of ABC Random Forest.

Deciphering invasion routes from molecular data is crucial to understanding biological invasions, including identifying bottlenecks in population size and admixture among distinct populations. Here, we unravel the invasion routes of the invasive pest Drosophila suzukii using a multi-locus microsatellite dataset (25 loci on 23 worldwide sampling locations). To do this, we use approximate Bayesian computation (ABC), which has improved the reconstruction of invasion routes, but can be computationally expensive.

Atypical scrapie in Australia.

Background: Since its initial detection in Norway in 1998, atypical scrapie ('atypical/Nor98 scrapie') has been reported in sheep in the majority of European countries (including in regions free of classical scrapie) and in the Falkland Islands, the USA, Canada, New Zealand and Australia.

Case Series: The diagnosis in Australia of atypical scrapie in four Merino and one Merino-cross sheep showing clinical signs of neurological disease was based on the detection of grey matter neuropil vacuolation (spongiform change) in the brain (particularly in the molecular layer of the cerebellar cortex) and associated abnormal prion protein (PrP ) deposition in both grey and white matter. Changes were minimal in the caudal brainstem, the predilection site for lesions of classical scrapie.

Local near instantaneously dynamically triggered aftershocks of large earthquakes.

Aftershocks are often triggered by static- and/or dynamic-stress changes caused by mainshocks. The relative importance of the two triggering mechanisms is controversial at near-to-intermediate distances. We detected and located 48 previously unidentified large early aftershocks triggered by earthquakes with magnitudes between ≥7 and 8 within a few fault lengths (approximately 300 kilometers), during times that high-amplitude surface waves arrive from the mainshock (less than 200 seconds).

Clinical, diagnostic and pathologic features of presumptive cases of Chlamydia pecorum-associated arthritis in Australian sheep flocks.

Background: Arthritis is an economically significant disease in lambs and is usually the result of a bacterial infection. One of the known agents of this disease is Chlamydia pecorum, a globally recognised livestock pathogen associated with several diseases in sheep, cattle and other hosts. Relatively little published information is available on the clinical, diagnostic and pathologic features of C.

population response to environment and management strategies.

causes economic damage to berry and stone fruit worldwide. Laboratory-generated datasets were standardized and combined on the basis of degree days (DD), using Gompertz and Cauchy curves for survival and reproduction. Eggs transitioned to larvae at 20.

Constraining Solar Wind Heating Processes by Kinetic Properties of Heavy Ions.

We analyze the heavy ion components (A>4  amu) in collisionally young solar wind plasma and show that there is a clear, stable dependence of temperature on mass, probably reflecting the conditions in the solar corona. We consider both linear and power law forms for the dependence and find that a simple linear fit of the form T_{i}/T_{p}=(1.35±.

Seasonal cues induce phenotypic plasticity of Drosophila suzukii to enhance winter survival.

Background: As global climate change and exponential human population growth intensifies pressure on agricultural systems, the need to effectively manage invasive insect pests is becoming increasingly important to global food security. Drosophila suzukii is an invasive pest that drastically expanded its global range in a very short time since 2008, spreading to most areas in North America and many countries in Europe and South America. Preliminary ecological modeling predicted a more restricted distribution and, for this reason, the invasion of D.

Attraction of the Invasive Halyomorpha halys (Hemiptera: Pentatomidae) to Traps Baited with Semiochemical Stimuli Across the United States.

A recent identification of the two-component aggregation pheromone of the invasive stink bug species, Halyomorpha halys (Stål), in association with a synergist, has greatly improved the ability to accurately monitor the seasonal abundance and distribution of this destructive pest. We evaluated the attraction of H. halys to black pyramid traps baited with lures containing the pheromone alone, the synergist methyl (2E,4E,6Z)-decatrienoate (MDT) alone, and the two lures in combination.

The Effects of Kernel Feeding by Halyomorpha halys (Hemiptera: Pentatomidae) on Commercial Hazelnuts.

Halyomorpha halys Stål, the brown marmorated stink bug (Hemiptera: Pentatomidae), is an invasive pest with established populations in Oregon. The generalist feeding habits of H. halys suggest it has the potential to be a pest of many specialty crops grown in Oregon, including hazelnuts, Corylus avellana L.