Adaptation of human long-term B lymphoblastoid cell lines to chemically defined, serum-free media.

Attempts were made to adapt human long-term B lymphoblastoid cell lines to prolonged growth in serum-free, chemically defined media. A newly described medium, which is an enriched modification of Dulbecco's modified Eagle's medium containing additional amino acids and vitamins, was used. The serum is totally replaced by albumin, transferrin, and soybean lipid.

Quantitative model for gene regulation by lambda phage repressor.

A statistical thermodynamic model has been developed to account for the cooperative interactions of the bacteriophage lambda repressor with the lambda right operator. The model incorporates a general theory for quantitatively interpreting cooperative site-specific equilibrium binding data. Values for all interaction parameters of the model have been evaluated at 37 degrees C, 0.

The effect of diltiazem on coronary thrombosis in the conscious canine.

The effect of diltiazem vs. saline was studied in a conscious canine model of coronary thrombosis. Diltiazem given as a 0.

Invasive fungal infections in patients with chronic mucocutaneous candidiasis.

Patients with chronic mucocutaneous candidiasis have persistent Candida infection of nails, skin, and mucous membranes, but rarely, if ever, does an invasive fungal infection develop. We describe two patients with chronic mucocutaneous candidiasis who died of fungal infection; one had C albicans meningitis and the second had Cryptococcus neoformans meningitis. The cases of four other patients with chronic mucocutaneous candidiasis and invasive fungal infection, three with crytococcosis and one with histoplasmosis, have been reported previously.

Experimental bacterial keratitis: a quantitative model of leukocyte migration following transfusion.

A model for the study of polymorphonuclear leukocyte (PMN) migration after transfusion employing induction of keratitis in guinea pigs was developed. Initial studies demonstrated that compared with other agents, intracorneal injection of Pseudomonas aeruginosa following in vivo labelling of PMN by administration of 3H-thymidine produced the greatest influx of radiolabelled PMN into corneas. In subsequent studies, donor peritoneal PMN were radio-labelled by injection of donors with 3H-thymidine.

A genetic defect of granulocyte oxidative metabolism in a man with disseminated aspergillosis.

A 26-year-old man presented with progressive pneumonia, and Aspergillus was grown from cultures of lung, cutaneous nodules, and urine. His PMNs had a poor CL response after exposure to phagocytic stimuli (S. aureus, latex, aggregated IgG and IgG-coated latex) (p less than 0.

The use of Silastic in tympanoplasty surgery.

Silastic has a relatively high incidence of late complications when used as a middle ear liner in unstaged tympanoplasty procedures. This does not seem to be the case in staged procedures when it is removed at the second operation or in cases where small pieces are used to prevent refixation of the malleus or stapes. At the present time, the use of gelfilm seems to be the most acceptable alternative.

Ciliary body reattachment in ocular hypotony.

Ocular hypotony associated with detachment of the ciliary body without evident cyclodialysis has been corrected by reattachment of the ciliary body to the sclera. This was achieved by suturing the ciliary body to sclera under microscopic control. It would seem that prolonged hypotony of this type can be cured by this method.

Giant cell lesions of the temporal bone.

Giant cell lesions are rare in the temporal bone. A review of world literature reveals twenty-three reported cases. Histopathologic differentiation has been difficult in the past; several tumors of bone which are characterized by multinucleated giant cells have been grouped together under the term "giant cell tumor.

Pars plasma vitrectomy in the management of the Irvine-Gass syndrome.

Twelve eyes of 10 patients with type I Irvine-Gass syndrome underwent pars plana vitrectomy after cystoid macular edema had been present a mean of 13 months and showed no evidence of resolving spontaneously or, in some cases, of responding to laser photocoagulation or steroid therapy. The disorder appears to resolve spontaneously in an average of 16 months. Following vitrectomy the edema resolved clinically in 10 of the 12 eyes, in an average of 3.

Phasic changes in bone CO2 fractions, calcium, and phosphorus during chronic hypercapnia.

The bone CO2 buffering system and bone calcium and phosphorus were studied in guinea pigs exposed to 1% CO2 for periods up to 8 wk and killed at weekly intervals together with control animals of the same age. Measurements were made of arterial CO2 tension, pH, standard bicarbonate, and bone Ca and P. Heat-stabile bone CO2 (carbonate) was determined as dry bone CO2 and heat-labile bone CO2 (bicarbonate) as delta wet-dry bone CO2.

Determination of posttransfusion granulocyte kinetics by chemiluminescence in chronic granulomatous disease.

A 14 year old boy with CGD of childhood received HLA-matched granulocyte transfusions in the therapy of an intramural abscess of the ileum. Donor granulocyte survival after transfusion was determined with the endotoxin-stimulated NBT test as well as an assay of chemiluminescence employing whole blood. The presence of circulating donor PMNs after transfusion was documented by both techniques.

The surgical management of macular pucker in rhegmatogenous retinal detachment.

Fourteen patients underwent surgery for macular pucker. The maximal follow-up is 26 months, the minimum is 3 months, and the average is 10 months. The essential feature of the operation is mechanical separation of the posterior cortical vitreous from the retinal surface.

Bone CO2-titration curves in acute hypercapnia obtained with a modified titration technique.

Bone CO2-titration curves were obtained in mature rats weighing 500-600 g. Animals were exposed for one hour to 1, 3, 5, 10, or 15% CO2 in air. Measurements of bone CO2, were made using a modified titrimetric analysis on fresh and oven-dried samples of paired rat femurs.

Acoustic neuroma removal: a comparative study of translabyrinthine and suboccipital approaches.

A comparative study of the suboccipital and translabyrinthine approaches for acoustic neuroma removal in 91 cases using a otologic-neurosurgical team is presented. Fifty of these cases involved the suboccipital route using microsurgical techniques; the remaining cases involved the standard otologic routes. The same neurosurgeon participated in all 91 cases.

The surgical management of macular pucker.

Contractions of collagenous membranes in the posterior cortical vitreous may produce macular pucker and loss of central vision. It is possible to separate these membranes from the retina, and if one succeeds in doing so there is reasonable improvement in central visison. Between December 1976 and September 1978, six patients with macular pucker were operated upon.

Effect of prolonged exposure to 0.5% CO2 on kidney calcification and ultrastructure of lungs.

Guinea pigs were exposed for up to 8 weeks to 0.5% CO2, 21% O2, and balance N2. Control groups of the same age were kept simultaneously in environmental chambers on air.

Comparison of product moment and rank correlation coefficients in the assessment of laboratory method-comparison data.

We have studied the effects of range and distribution of data on product moment and rank correlation coefficients when deviation from a linear relationship was due solely to experimentally produced random error. All correlation coefficients (Pearson r, Spearman rho, and Kendall tau) were markedly influenced by the range of the data, and, for the rank correlation coefficients, the effect of range varied for different data distributions. While correlation coefficients may be useful in assessing whether an association exists between two variables, they are not useful in assessing the degree of random error about the regression line when a strong linear association is presumed to exist between the two variables.

AO incompatibility in a small group of developmentally disabled children.

Prevalence of fetal-maternal AO incompatibility was studied in a group of 150 children having developmental delay in the neuropsychological sphere. In a given population the number of A children having O mothers (AO incompatibility) should equal the number of O children having A mothers (OA compatibility). However, in the study group we observed 26 children in the former and 14 children in the latter category.

Postinflammatory osteogenic fixation of the stapes.

Stapes fixation existing with chronic otitis media has been attributed usually to either tympanosclerosis or concomitant otosclerosis. A different type of stapes fixation has been encountered which the author has termed "post-inflammatory osteogenic stapes fixation." One thousand five hundred and two surgical procedures for chronic otitis media were reviewed with 112 rigid stapes fixations found of which 19 were classified as post-inflammatory osteogenic stapes fixation.

Vitrectomy in retinal detachment surgery.

Pars plana vitrectomy has provided an approach to those situations in which treatment of a retinal detachment becomes impossible due to vitreous pathology. The records of the first 207 pars plana vitrectomies performed by one of us (M.S.

Pars plana vitrectomy.

We describe our first 127 vitrectomies through the pars plana performed on 120 eyes for a variety of indications. The success rate in anterior segment disorders was 45% and in those of the posterior segment 34%. While in some cases the results were encouraging, in others they were disastrous.