[Strength properties of rabbit cornea after femtosecond laser keratoplasty with and without different intracorneal implants].

Aim: to conduct a comparative experimental study of the effect of the use of intracorneal implants during femtosecond laser-assisted intrastromal pocket (IP) and intrastromal tunnel (IT) formation on the strength properties of the cornea.

Material And Methods: The experimental study was performed on 24 corneas of 12 rabbit eyes enucleated 1 month after slaughtering. All eyes were divided into 6 groups according to the method of operation.

[A biotechnical system for diagnosis and treatment of binocular vision impairments].

Automation of the binocular vision biorhythm diagnosis and improvement of the efficacy of treatment of vision impairments are important medical problems. In authors' opinion, to solve these problems, it is necessary to take into account the correlation between the binocular vision and the electrical activity of the brain. A biotechnical system for diagnosis and treatment of binocular vision impairments was developed to implement diagnostic and treatment procedures based on the detection of this correlation.

["Stationary phase aging" of cell culture: an attempt of evaluation of growth medium "age" effect].

Cell proliferation rate and 3H-thymidine labeling index of "young" (i. e. harvested in 3 days after subcultivation) cultured Chinese hamster cells (B11 dii-FAF28 line) have been determined in growth medium conditioned by the same cells for various periods of time during their growth and subsequent "stationary phase aging" (medium of different "age").

[Study of protein products of gene expression in cells with altered chromosome sets for genetic mapping].

Two-dimensional electrophoresis was used for analyzing proteins in hybrid cells that contained single human chromosomes (chromosome 5, chromosome 21, or chromosomes 5 and 21) against the background of the mouse genome. By comparing the protein patterns of hybrid and parent cells (about 1000 protein fractions for each kind of cell), five fractions among proteins of hybrid cells were supposedly identified as human proteins. The genes of two of them are probably located on chromosome 5, and those of other three, on chromosome 21.

[Erythrocyte membrane proteins in patients with hereditary spherocytosis].

Red blood cell membrane proteins were studied in a group of patients with hereditary spherocytosis, in comparison with normal donors, to reveal anomalous proteins associated with this disease. For this purpose red blood cells of the patients and normal donors were fractionated, by the age, in Ficoll-400 gradient, as a result red blood cell membranes were obtained with proteins that were investigated by the method of two-dimensional electrophoresis. In comparison of two-dimensional electrophoregrams of red blood cell membrane proteins of normal donors and those of microspherocytosis patients it was found that the latters had additional peptides in the area of glyceraldehyde-3-phosphate hydrogenase and pyruvate kinase.

[Comparative study of human erythrocyte membranes in normal people and in Huntington's chorea patients].

Erythrocytes of healthy volunteers and of patients with hereditary chorea were studied. Evaluation of the state of cellular membrane was carried out by measuring osmotic resistance, activity of Na+, K(+)-ATPase and protein composition. In the patients osmotic resistance of erythrocytes was distinctly decreased down to 66.

[Directed screening of bacteria--destructive agents of surface-active substances].

A procedure for detection of bacteria destructing cationic surface active substances (CSAS) was developed. The procedure is based on changing colour of pyrocatechin violet, an organic dye from geen-blue in the presence of a CSAS to red-brown in its absence. After application of pyrocatechin violet to bacterial macrocolonies grown on a synthetic medium with a CSAS as the only source of C red-brown zones developed around the colonies which was probably indicative of the CSAS biodestruction.

[Properties of a toxic substance from Erwinia carotovora var. citrullis].

The toxic substance of Erwinia carotovora, isolated in a pure form, is a protein-polysaccharide complex with molecular weight of 90000 daltons. The protein part of the complex contains 17 amino acids with the prevalence of dicarbonic acids and with insignificant quantities of thyrosine and phenylalanine, while the polysaccharide part of the complex contains 6 sugars. The antigenic specificity of the complex is linked with its hydrocarbon part, and the toxicity of the complex is determined by protein.

[Effect of digitonin on the activity of Na,K-ATPase from bovine brain].

The kinetic properties of intact and digitonin-treated Na,K-ATPase from bovine brain were studied. The temperature dependence curve for the rate of ATP hydrolysis under optimal conditions (upsilon 0) in the Arrhenius plots shows a break at 19-20 degrees. The temperature dependence curves for Km' and Km" have breaks at the same temperatures, while the Arrhenius plot for V is linear.