Publications by authors named "Shazia Ameen"
Int J Biol Macromol
May 2024
Article Synopsis
- The AcPase enzyme has a high specific activity of 31.32 U/mg with significant purification and a low yield of 3.15% compared to the Zn-dependent variant which has a lower specific activity of 14 U/mg and higher total recovery at 5.14%.
- SDS-PAGE analysis shows a single band for each enzyme, with AcPase at 18 kDa and Zn-AcPase at 29 kDa, indicating their molecular weights.
- AcPase shows broad substrate specificity, while Zn-AcPase shows preference for certain substrates, and various divalent metal ions enhance enzyme activity, while others inhibit it; competitive inhibitors have been identified for both enzyme types,
