Role of polymorphisms in pathophysiology of Crohn's disease.

Aim: To establish the relationship of () polymorphisms and mycobacterial infections in Crohn's disease (CD).

Methods: All 133 subjects' blood samples were genotyped for nine single nucleotide polymorphisms (SNPs) in using TaqMan™ genotyping, while the effect of the SNPs on and γ gene expression was determined using RT-PCR. Detection of subspecies (MAP) gene was done by nPCR after DNA extraction from the isolated leukocytes of each subjects' blood samples.

Polymorphisms in Are Linked to Hyper-Proliferative T-Cells and Susceptibility to in Rheumatoid Arthritis.

A shared genetic pre-disposition, chronic inflammation, and treatment with similar biologics between Rheumatoid arthritis (RA) and Crohn's disease (CD) have intrigued us to investigate whether the two disorders share trigger association or possible causation. We hypothesized earlier that Single Nucleotide Polymorphisms (SNPs) in the negative regulators ( lead to a dysregulated immune response, susceptibility to environmental triggers, and continued apoptosis as seen in chronic inflammation in RA and CD. To test the hypothesis, peripheral leukocytes samples from 132 consented subjects were genotyped for 9 SNPs in /22 using TaqMan™ genotyping.