3D structure of phosphofructokinase from Pichia pastoris: Localization of the novel gamma-subunits.

The largest and one of the most complex ATP-dependent allosteric phosphofructokinase (Pfk) has been found in the methylotrophic yeast, Pichia pastoris. The enzyme is a hetero-oligomer ( approximately 1MDa) composed of three distinct subunits (alpha, beta and gamma) with molecular masses of 109, 104 and 41kDa, respectively. While the alpha- and beta-subunits show sequence similarities to other phosphofructokinase subunits, the gamma-subunit does not show high homology to any known protein in the databases.

Structures of S. pombe phosphofructokinase in the F6P-bound and ATP-bound states.

Phosphofructokinase (Pfk1; EC 2.7.1.