Sustainable Approach for Degradation of Low-Density Polyethylene Plastic Waste Using Ligninolytic White Rot Fungus.

Bisphenol A (BPA), an endocrine disruptor is used in manufacturing of polycarbonate plastics for food-drink packaging. In the present study, optimized set of conditions to degrade commercial grade BPA has been used and applied in degrading shredded leached low-density polyethylene (LDPE) residues and its leachate (198 µg/L BPA) using white rot fungus Hypocrea lixii. One-at-a-time method showed maximum BPA degradation of 98.

Evaluation of an Electrochemiluminescence Assay for the Rapid Detection of Ricin Toxin.

In this article, we detail a comprehensive laboratory evaluation of an immunoassay for the rapid detection of ricin using the Meso Scale Diagnostics Sector PR2 Model 1800. For the assay evaluation, we used inclusivity, exclusivity, and informational panels comprised of extracts of 35 near-neighbor plant cultivar-extracts, 66 lectins, 26 white powders, 16 closely related toxins and proteins/toxoids, and a pool of 30 BioWatch filter extracts. The results show that the Meso Scale Diagnostics ricin detection assay exhibits good sensitivity and specificity with a limit of detection of 1.

Solubilization and enhanced degradation of benzene phenolic derivatives-Bisphenol A/Triclosan using a biosurfactant producing white rot fungus S5 with plant growth promoting traits.

Introduction: Endocrine disrupting chemicals (EDCs) as benzene phenolic derivatives being hydrophobic partition to organic matter in sludge/soil sediments and show slow degradation rate owing to poor bioavailability to microbes.

Methods: In the present study, the potential of a versatile white rot fungal isolate S5 identified as was monitored to degrade bisphenol A (BPA)/triclosan (TCS) under shake flask conditions with concomitant production of lipopeptide biosurfactant (BS) and plant growth promotion.

Results: Sufficient growth of WRF for 5 days before supplementation of 50 ppm EDC (BPA/TCS) in set B showed an increase in degradation rates by 23% and 29% with corresponding increase in secretion of lignin-modifying enzymes compared to set A wherein almost 84% and 97% inhibition in fungal growth was observed when BPA/TCS were added at time of fungal inoculation.

Structural and genomic evolutionary dynamics of Omicron variant of SARS-CoV-2 circulating in Madhya Pradesh, India.

The SARS-CoV-2 Omicron (B.1.1.

Fatigue analysis of ambient-cured geopolymer concrete for high-traffic pavements.

The building sector is growing at a rapid rate, leading to an increased demand for construction materials. Concrete made with Ordinary Portland Cement (OPC) has long been the preferred choice due to its excellent bonding properties and versatility as demanded by construction process. However, the manufacturing of Ordinary Portland Cement (OPC) leads to negative impacts on the environment, with the cement sector responsible for around 5-8% of global CO emissions.

Split X-Jaw techniques of volumetric modulated arc radiotherapy in nasopharyngeal cancer: A dosimetric comparison.

Purpose: The current study aims to compare the split x-jaw planning technique of volumetric modulated arc radiotherapy (VMAT) with the traditional open and limited jaw techniques of VAMT in nasopharyngeal carcinoma treatment. The multi-leaf collimators on the varian linear accelerator move on a carriage with a maximum leaf span of 15 cm. Therefore, treatment of larger planning target volumes, such as in nasopharyngeal cancer with traditional open and limited jaw technique, yields compromised dose distribution.

A highly selective coumarin-based chemosensor for dual sensing of Cu and Zn ions with logic gate integration and live cell imaging.

In this paper, a coumarin-based Schiff base chemosensor has been synthesized and developed to detect Cu and Zn ions in nanomolar concentrations. The probe selectively distinguishes Cu and Zn from among several metal ions in DMF : HO (7 : 3, v/v, pH 7.4) HEPES buffer.

Composites of sodium alginate based - Functional materials towards sustainable adsorption of benzene phenol derivatives - Bisphenol A/Triclosan.

The present study evaluates the adsorption efficiency of low-cost carbonaceous adsorbents as fly ash (FA), saw dust biochar (SDB) (untreated and alkali - treated), live/dead pulverized white rot fungus Hypocrea lixii biomass encapsulated in sodium alginate (SA) against the commercially available activated carbon (AC) and graphene oxide (GO) SA beads for removal of benzene phenol derivatives - Bisphenol A (BPA)/triclosan (TCS). Amongst bi - and tri - composites SA beads, tri-composite beads comprising of untreated flyash - dead fungal biomass - sodium alginate (UFA - DB - SA) showed at par results with commercial composite beads. The tri - composite beads with point zero charge (P) of 6.

Closed genome sequence of type B1 strain isolated from an infant botulism case in the United States.

We present the closed genome sequence of the strain isolated from the stool specimen of an infant diagnosed with botulism. With 4.33-Mb genome size and 28.

Analytical parameters to check association between load carriage system and bag packs-influenced musculoskeletal complications among school going students: a scoping review.

Purpose: This study aimed to investigate the correlation between load-carriage systems and backpacks on musculoskeletal complications among school-going students. Additionally, the purpose was to identify research gaps and provide recommendations for future research.

Methods: To conduct this study, we conducted a thorough search of five databases for literature published between January 2010 and December 2022.

Special Issue: Gram-Positive Bacterial Toxins.

The Gram stain classifies most bacteria into one of two groups, Gram-negative or Gram-positive, based on the composition of their cell walls [...

draft genome sequences: 15 historical (1998-2004) and 30 contemporary (2015-2016) clinical isolates from Pakistan.

The continued emergence and spread of antimicrobial resistance among pathogenic bacteria are ever-growing threats to health and economy. Here, we report the draft genomes for 45 clinical isolates, including historical and contemporary drug-resistant organisms, obtained in Pakistan between 1998 and 2016: 5 , 3 , 3 , and 34 .

Simplified visual detection of Kyasanur Forest Disease virus employing Reverse Transcriptase-Polymerase Spiral Reaction (RT-PSR).

Among recently prevalent tick-borne infections in India, Kyasanur Forest Virus Disease (KFD) is an important public health concern. During last decade the emergence of cases apart from endemic zone raised concern about case positivity. Early diagnosis is therefore very important in disease management and primary containment.

Isolation and characterization of emerging Mpox virus from India.

Mpox (previously known as Monkeypox) has recently re-emerged, primarily through human-to-human transmission in non-endemic countries including India. Virus isolation is still considered as the gold standard for diagnosis of viral infections. Here, the qPCR positive skin lesion sample from a patient was inoculated in Vero E6 cell monolayer.

Peutz-Jeghers Syndrome: Lessons to be Learned in the Clinical Diagnosis.

Introduction: Peutz-Jeghers Syndrome (PJS) is an autosomal dominant disease presenting with hamartomatous polyps in the gastrointestinal tract and hyperpigmented macules on lips and oral mucosa. The incidence of this syndrome is approximately 1 in 1,20,000 births.

Materials And Methods: In this article, we are presenting 11 cases of PJS which were misdiagnosed and patients were compelled to visit hospital repeatedly.

Dry- down probe free qPCR for detection of KFD in resource limited settings.

Kyasanur Forest Disease is a tick-borne flavivirus is endemic in the Southern India. The recent expansion and resurgence of sporadic outbreaks in southern parts of country is the most important concern. Although only formalin inactivated vaccine is available for treatment with limited efficacy the early detection and timely identification is a only way to prevent spread of cases.

Hernia of Umbilical Cord: An Enigma.

Introduction: Hernia of the umbilical cord (HUC) is an uncommon problem which is often misdiagnosed, leading to inadequate treatment and various complications which cause increased morbidity and mortality in neonates. To address this issue, we took up this study.

Materials And Methods: Patients diagnosed with HUC from January 1, 2017, to December 31, 2021, were retrospectively analyzed.

Molecular Mapping of Biofortification Traits in Bread Wheat ( L.) Using a High-Density SNP Based Linkage Map.

A set of 188 recombinant inbred lines (RILs) derived from a cross between a high-yielding Indian bread wheat cultivar HD2932 and a synthetic hexaploid wheat (SHW) Synthetic 46 derived from tetraploid (AA, BB 2n = 28) and diploid (DD, 2n = 14) was used to identify novel genomic regions associated in the expression of grain iron concentration (GFeC), grain zinc concentration (GZnC), grain protein content (GPC) and thousand kernel weight (TKW). The RIL population was genotyped using SNPs from 35K Axiom Wheat Breeder's Array and 34 SSRs and phenotyped in two environments. A total of nine QTLs including five for GPC (, , , , and ), two for GFeC ( and ), and one each for GZnC () and TKW () were identified.

Synthesis, Structural Investigations, and In Vitro/In Silico Bioactivities of Flavonoid Substituted Biguanide: A Novel Schiff Base and Its Diorganotin (IV) Complexes.

Quercetin is one of the most powerful bioactive dietary flavonoids. The in vivo biological study of quercetin is extremely difficult due to its very low solubility. However, diorganotin complexes of quercetin are more useful when contrasted with quercetin due to increased solubility.

Antimicrobial screening to molecular docking of newly synthesized ferrocenyl-substituted pyrazole.

Objective: Microbial diseases are snowballing at an alarming proportion. Therefore, the intent of this study was to inspect the antimicrobial action of ferrocenyl-substituted pyrazole against various human pathogenic Gram-positive, Gram-negative, and fungal microbial strains. Pyrazoles have been recognized for over a century as a significant and bioactive class of heterocyclic compounds.

A comprehensive account of SARS-CoV-2 genome structure, incurred mutations, lineages and COVID-19 vaccination program.

This review collates information on the onset of COVID-19, SARS-CoV-2 genome architecture, emergence of novel viral lineages that drove multiple waves of infection around the world and standard and fast track development of vaccines. With the passage of time, the continuously evolving SARS-CoV-2 has acquired an expanded mutational landscape. The functional characterization of spike protein mutations, the primary target of diagnostics, therapeutics and vaccines has revealed increased transmission, pathogenesis and immune escape potential in the variant lineages of the virus.