Extracellular vesicles as therapeutic tools in regenerative dentistry.

Dental and maxillofacial diseases are always accompanied by complicated hard and soft tissue defects, involving bone, teeth, blood vessels and nerves, which are difficult to repair and severely affect the life quality of patients. Recently, extracellular vesicles (EVs) secreted by all types of cells and extracted from body fluids have gained more attention as potential solutions for tissue regeneration due to their special physiological characteristics and intrinsic signaling molecules. Compared to stem cells, EVs present lower immunogenicity and tumorigenicity, cause fewer ethical problems, and have higher stability.

CCL17, CCL22 and their receptor CCR4 in hematologic malignancies.

Hematological malignancies (HM) are common malignant tumors with high morbidity and mortality rates, and are malignant diseases that seriously affect human health, with chemotherapy prone to recurrence and toxic side effects. Therefore, the development of precise, effective, and safe targeted therapeutic agents has become a hotspot in the current research of antitumor technology. More and more studies have shown that the interaction of C-C chemokine ligand 17 (CCL17) and C-C chemokine ligand 22 (CCL22) with the receptor C-C chemokine receptor type 4 (CCR4) promotes the immune escape of tumors and is closely related to the occurrence, development, and prognosis of hematological tumors.

Complete Global Total Electron Content Map Dataset based on a Video Imputation Algorithm VISTA.

Ionospheric total electron content (TEC) derived from multi-frequency Global Navigation Satellite System (GNSS) signals and the relevant products have become one of the most utilized parameters in the space weather and ionospheric research community. However, there are a couple of challenges in using the global TEC map data including large data gaps over oceans and the potential of losing meso-scale ionospheric structures when applying traditional reconstruction and smoothing algorithms. In this paper, we describe and release a global TEC map database, constructed and completed based on the Madrigal TEC database with a novel video imputation algorithm called VISTA (Video Imputation with SoftImpute, Temporal smoothing and Auxiliary data).

Global Driving of Auroral Precipitation: 1. Balance of Sources.

The accurate determination of auroral precipitation in global models has remained a daunting and rather inexplicable obstacle. Understanding the calculation and balance of multiple sources that constitute the aurora, and their eventual conversion into ionospheric electrical conductance, is critical for improved prediction of space weather events. In this study, we present a semi-physical global modeling approach that characterizes contributions by four types of precipitation-monoenergetic, broadband, electron, and ion diffuse-to ionospheric electrodynamics.

Exosomes released from Sertoli cells contribute to the survival of Leydig cells through CCL20 in rats.

Reciprocal communication between Sertoli and Leydig cells occurs in the testes; however, the detailed mechanisms involved are not completely understood. Exosomes can communicate within neighboring or distant cells to regulate cell function. Our aim was to determine whether exosomes released from Sertoli cells can regulate the survival of Leydig cells.

Comparison of intracytoplasmic sperm injection (ICSI) outcomes in infertile men with spermatogenic impairment of differing severity.

The extent of spermatogenic impairment on intracytoplasmic sperm injection (ICSI) outcomes and the risk of major birth defects have been little assessed. In this study, we evaluated the relationship between various spermatogenic conditions, sperm origin on ICSI outcomes, and major birth defects. A total of 934 infertile men attending the Center for Reproductive Medicine of Ren Ji Hospital (Shanghai, China) were classified into six groups: nonobstructive azoospermia (NOA; n = 84), extremely severe oligozoospermia (esOZ; n = 163), severe oligozoospermia (sOZ, n = 174), mild oligozoospermia (mOZ; n = 148), obstructive azoospermia (OAZ; n = 155), and normozoospermia (NZ; n = 210).

Seminal exosomal miR-210-3p as a potential marker of Sertoli cell damage in Varicocele.

Background: Varicocoele-associated stressors, such as hypoxia and heat, can damage cell function and viability, and some exosomal biomarkers released from impaired cells may reflect the cell status in testis.

Objectives: To find if seminal exosomal microRNAs can reflect the Sertoli cell function in varicocoele.

Materials And Methods: Experimental left varicocoele rat model was established (n = 24), and patients with different grades of varicocoele (n = 104) were enrolled.

Nitrifying biomass can retain its acclimation to 2,4,6-trichlorophenol.

Many municipal wastewater treatment plants in China receive industrial wastewater that contains inhibitory organic chemicals, such as chlorinated phenols. For the common aerobic biological treatment, nitrification is a key step, but nitrifying bacteria are notably sensitive to inhibition by chlorinated phenols. In this work, normal activated sludge (containing nitrifying biomass) was acclimated to 2,4,6-trichlorophenol (TCP).

Comparison and outcomes of nonobstructive azoospermia patients with different etiology undergoing MicroTESE and ICSI treatments.

Background: The purpose of the study was to compare clinical, laboratory, histological features, microdissection testicular sperm extraction (MicroTESE) and intracytoplasmic sperm injection (ICSI) treatment outcomes of nonobstructive azoospermia (NOA) patients of various etiologies, and to investigate ICSI outcomes using fresh and frozen thawed sperms from MicroTESE, so to explore an optimal MicroTESE-ICSI procedure for NOA couples.

Methods: A retrospective analysis was made in 595 NOA patients undergoing MicroTESE from January 2013 to December 2017. The men were classified into six groups based on etiology.

Bioavailable electron donors from ultrasound-treated biomass for stimulating denitrification.

Finding low-cost electron donors to drive denitrification is an important target for many municipal wastewater treatment plants (MWTPs). Excess sludge (biomass) potentially is a low-cost electron donor generated internally to the MWTP, but it has to be made more bioavailable. Aerobic and anoxic biomasses were treated with ultrasound, and their supernatants were used as electron donors for stimulating denitrification.

Acclimation of nitrifying biomass to phenol leads to persistent resistance to inhibition.

It is common that biological wastewater-treatment processes are exposed to inputs of toxic compounds, such as phenolics. Due to their slow growth rate, nitrifying bacteria are most susceptible to inhibition that can lead to loss of nitrification capacity. Here, a microbial community containing nitrifying bacteria was acclimated to phenol, and it developed resistance to phenol inhibition and maintained nitrification activity.

Simultaneous anaerobic and aerobic transformations of nitrobenzene.

Aerobic biodegradation of nitrobenzene (NB) produces nitrophenol (NP), which has stronger toxicity than NB. Anaerobic biodegradation of NB produces aniline (AN), which has weaker toxicity, but is a dead-end product in anaerobic conditions. Accumulation of AN should be overcome by coupling anaerobic and aerobic transformations: NB is transformed to AN in an anaerobic zone of the bioreactor, and AN is then transformed in an aerobic zone.

Association of a TDRD1 variant with spermatogenic failure susceptibility in the Han Chinese.

Purpose: Piwi-interacting RNAs (piRNAs) are a broad group of noncoding small RNAs that have important biological functions in germline cells and can maintain genome integrity via silencing of retrotransposons. In this study, we aimed to explore the associations between genetic variants of important genes involved in piRNA biogenesis and male infertility with spermatogenic impairment.

Methods: To this end, five single-nucleotide polymorphisms (SNPs) in the ASZ1, PIWIL1, TDRD1, and TDRD9 genes were genotyped by TaqMan allelic discrimination assays in 342 cases of nonobstructive azoospermia (NOA) and 493 controls.

A novel micro-straw for cryopreservation of small number of human spermatozoon.

Cryopreservation of few spermatozoa is still a major challenge for male fertility preservation. This study reports use a new micro-straw (LSL straw) for freezing few spermatozoa for intracytoplasmic sperm injection (ICSI). Semen samples from 22 fertile donors were collected, and each semen sample was diluted and mixed with cryoprotectant in a ratio of 1:1, and then frozen using three different straws such as LSL straw (50-100 μl), traditional 0.

VEGF/VEGFR2 Signaling Regulates Germ Cell Proliferation in vitro and Promotes Mouse Testicular Regeneration in vivo.

Vascular endothelial growth factor (VEGF) plays fundamental roles in testicular development; however, its function on testicular regeneration remains unknown. The objective of this study was to explore the roles VEGF/VEGFR2 signaling plays in mouse germ cells and in mouse testicular regeneration. VEGF and the VEGFR2 antagonist SU5416 were added to culture medium to evaluate their effects on spermatogonial stem cell line (C18-4 cells) proliferation.

Endothelial nitric oxide synthase (eNOS) T-786C, 4a4b, and G894T polymorphisms and male infertility: study for idiopathic asthenozoospermia and meta-analysis.

Recent studies on the eNOS gene and male infertility show that expression of eNOS regulates normal spermatogenesis in the testis, and the eNOS gene variants (T-786C, 4a4b, and G894T) are potentially involved in impairment of spermatogenesis and sperm function. Thus, we conducted this association and meta-analysis study to further validate whether variants of those three loci affected the risk of idiopathic asthenozoospermia (AZS) and male infertility. Approximately 340 Chinese idiopathic AZS patients and 342 healthy men were included for this case-control study, genotyped by gel electrophoresis analysis or direct sequencing of PCR products.

Mesenchymal stem cells overexpressing Ihh promote bone repair.

Background: Indian hedgehog (Ihh) signaling pathway is known to play key roles in various aspects of normal endochondral bone development. This study tested the potential roles of high Ihh signaling in the context of injury-induced bone regeneration.

Methods: A rabbit tibia defect model was established to test the effects of the implant of Ihh/mesenchymal stem cells (MSCs)/scaffold complex.

Association study between polymorphisms of PRMT6, PEX10, SOX5, and nonobstructive azoospermia in the Han Chinese population.

A previous genomewide association study of nonobstructive azoospermia (NOA) in the Han Chinese population identified three risk loci (rs12097821, rs2477686, and rs10842262) and provided strong evidence for a genetic influence in male infertility. However, recently, a follow-up study of these single nucleotide polymorphism (SNP) loci in the Japanese population showed that none of them were significantly associated with NOA. Therefore, we conducted an association study, consisting of 550 NOA cases and 555 normal controls, to further validate whether the risk of those three SNPs still existed in an independent Han Chinese male population.

Ectopic expression of Sonic Hedgehog in a cryptorchid man with azoospermia: a case report.

A 30-year-old man presented with a left undescended testis, right testicular deficiency and azoospermia. Testicular biopsy revealed an absence of spermatocytes and increased numbers of Leydig cells in the undescended testis. Additional comparative analyses were undertaken to explore Sonic Hedgehog (Shh) immunostaining in the testis of juvenile and adult mice, in the testis of the patient with cryptorchidism, and in archival testicular tissue from a patient with obstructive azoospermia and a patient with prostate cancer.

[Pre-freezing equilibration improves post-thaw human semen parameters].

Objective: To investigate the effect of pre-freezing equilibration on the cryo-survival of human sperm and to optimize the protocol of direct fumigation for the freeze-thawing of human sperm.

Methods: We collected 50 semen samples from healthy donors, each subjected to cryopreservation with 3 different methods: non-equilibration freezing (Group A), 10-min equilibration at room temperature before freezing (Group B), and 10-min equilibration at 4 degrees C before freezing (Group C). We examined all the post-thaw semen samples by computer-assisted semen analysis for the sperm motility parameters, and detected the sperm vitality and deformity index (SDI).

[Desert hedgehog regulates the proliferation and differentiation of Leydig cells: an update].

Hedgehog signaling pathway plays an important role in regulating the development of endocrine glands. Desert hedgehog (Dhh) has become a recent focus for its regulation of testis development, especially of Leydig cells. Dhh, as a Sertoli cell product, regulates the proliferation and differentiation of Leydig cell lineage and functions to secrete testosterone through a paracrine signaling mechanism.

[Reconstructed seminiferous tubules from ectopic grafting of testicular cells].

Objective: To investigate the ectopic grafts of mouse testicular cells by observing the reconstruction of seminiferous tubules, colonization of spermatogenic cells and spermatogenesis using immunodeficient mice as recipients.

Methods: The testes of newborn male ICR mice were digested to obtain single cell suspension. The cells were then mixed with matrigel and subcutaneously grafted into the dorsal region of the male nude mice.

Isolation, identification of Streptococcus pneumoniae from infected rhesus monkeys and control efficacy.

Background: Streptococcus pneumoniae can cause a wide variety of illnesses. Primate animals can be infected by the pneumococcus. A disease occurred among rhesus monkeys in winter 2006.