WEST NILE FEVER IN THE SARATOV REGION IN 2013-2015.

West Nile virus (WNV) circulation in the territory of Saratov region and its role in the infectious pathology were investigated. For this purpose, in studies conducted in 2013-2015, suspensions of bloodsucking arthropods, organs of birds and small mammals were analyzed for the presence of WNV markers (antigens and/or RNA). The seroprevalence level in live-stock animals and population of the region was evaluated; clinical samples from patients with symptoms compatible with West Nile fever (WNF) were analyzed.

[Formation of focus of West Nile fever on the territory of Saratov Region].

Aim: Detection of circulation of West Nile virus (WNV) on the territory of Saratov Region and prerequisites for formation of natural focus of West Nile fever (WNF), determination of the role of WNV in infectious pathology on the territory of the region.

Materials And Methods: of organs of small mammals, birds, blood-sucking arthropods for the presence of WNV markers (antigens and/or RNA) were studied. Clinical material from patients with symptoms not excluding WNF was studied.

[Detection of arbovirus antigens in the mosquitoes and ticks inhabiting the Saratov Region].

The paper gives the results of a study dealing with the detection of the antigens of arboviruses of West Nile, Sindbis, Batai, Crimean-Congo hemorrhagic fever, a serocomplex of Californian encephalitis in the field material gathered in the Saratov Region in 2000-2006. The bloodsucking arthropods inhabiting the region were shown to be actively involved in the circulation of arboviruses in natural biotopes. The conclusion that it is expedient to organize an annual monitoring of arbovirus-induced infections in the areas where positive findings have been notified is justified.

[ELISA and PCR test systems used to detect Crimean-Congo hemorrhagic fever virus].

Studying the sensitivity and specificity of enzyme-linked immunosorbent assay (ELISA) for the indication of Crimean-Congo hemorrhagic fever (CCHF) virus antigens and those of reverse transcription and polymerase chain reaction (RT-PCR) for the detection of CCHF virus RNA, and those of a intercerebral infection method in newborn albino mice systems for the determination of viral infectious activity established that the sensitivity of ELISA was 1-2 orders of magnitude less than that of RP-PCR. The latter proved to be better in studying the sera sampled from patients with CCHF. The results of studying the samples of H.

[Life cycles of ground beetles (Coleoptera, Carabidae) from the mountain taiga and mountain forest-steppe in the Eastern Sayan].

Seasonal dynamics and demographic structure was studied in 15 dominant ground beetle species in the mountain taiga and mountain forest-steppe belts of the Eastern Sayan (Okinskoe Plateau). Life cycles of the dominant ground beetle species were classified by developmental time, seasonal dynamics, and intrapopulation groups with different reproduction timing. The strategies of carabid life cycles adapted to severe mountain conditions of the Eastern Sayan were revealed.

[Features of the life cycles of Pterostichus montanus (Motschulsky, 1844) and Carabus loschnikovi (Fischer-Waldheim, 1822) (Coleoptera, Carabidae) in conditions of the mountain taiga belt in the Eastern Sayan].

We studied the seasonal dynamics and demographic structure of abundant ground beetle species from the mountain taiga belt in the Eastern Sayan. Data on the dynamics of the sexual and age structure of the populations as well as on the reproductive capacity of females in the biotopes on the slopes with different exposure and height were obtained. Life cycles with one- and two-year development were revealed for the ground beetles typical for the mountain taiga belt.

[Rotenone-insensitive NADH oxydation in mitochondrial suspension occurs by NADH dehydrogenase of respiratory chain fragments].

Two types of NADH oxidation, rotenone-sensitive and rotenone-insensitive, in suspension of beef heart mitochondria were investigated by the spectrophotometric method. The oxidation of the added NADH by mitochondria in hypotonic media occurs only through the NADH dehydrogenase of the respiratory chain, since it was totally blocked by rotenone or amytal (and also by antimycin A or azide), but the ferricyanide-activated NADH oxidation was insensitive to these inhibitors. The insensitivity of the NADH dehydrogenase to rotenone appears to be due to a shunt of the electron transfer to ferricyanide without involving of ubiquinone.

[Characterization of Vibrio cholerae eltor in the city of Kazan in 2001].

Information on V. cholerae eltor isolated in the focus of cholera in Kazan in 2001 at different periods of the outbreak is presented. The identity of strains isolated from patients, vibriocarriers and environmental objects, including their antibioticograms (sensitivity to cyprofloxacin and resistance to trimethoprim--sulfamethoxazole, streptomycin, furazolidone and nalidixic acid, which may be regarded as markers), is shown.

[Adaptive changes in fatty acid compositions of whitefish Coregonus lavaretus L. tissue lipids induced by anthropogenic factors].

Adaptive processes in the body of whitefish (Coregonus lavaretus L.) caused by anthropogenic effects on aquatic systems were studied. It was demonstrated that the content of fatty acid acyls correlated with the water pollution level.

[Intraoperative autohemotransfusion during correction of congenital heart defects].

Autohemotransfusion is one of the perspective methods of transfusion used in cardiosurgery. Intraoperative autohemotransfusion was used in surgical correction of congenital heart diseases in patients of various age groups, including children aged 6 to 8. The exfusion volume made up 300-600 ml, depending on the patient's body mass and the hematologic parameters.

Ultrastructural localization of HIV-1 RNA and core proteins. Simultaneous visualization using double immunogold labelling after in situ hybridization and immunocytochemistry.

The analysis of human immunodeficiency virus type 1 (HIV-1) RNA sequences in CEM and Jurkat lymphoid cells infected with the virus has been performed at the subcellular level. Using a biotinylated DNA probe specific for HIV-1, virus RNA sequences were detected on Lowicryl thin sections after immunogold cytochemistry. The labelling observed on the cytoplasm was localized near the plasma membrane connected with extracellular cluster of virions.

[Transfusion problems in artificial blood circulation].

Results of differentiated administration of blood components to cardiac surgical patients are presented. Frozen washed erythrocytes and erythromass were successively used in the perfusate and postoperatively. A transfusion pattern for patients with rare A2B blood group has been elaborated.

[Mutagenesis and cloning of hly determinants of the paP20 plasmid].

The data obtained indicate that pAP20 plasmid determinants for the synthesis and excretion of Escherichia coli haemolysin are located on the plasmid SalI-fragment f3. The size of f3 fragment is 12 X 10(6) dalton.

[Plasmid pAP20 controlling the hemolytic activity of E. coli].

The authors investigated pAP20 plasmid identified in E. coli cells isolated from man. According to the evidence obtained pAP20 plasmid determines the synthesis of alpha-hemolysin, being an F-like plasmid of the drd type.