Publications by authors named "Sharon J Keeler"
Elucidating the complex microbial interactions in biological environments requires the identification and characterization of not only the bacterial component but also the eukaryotic viruses, bacteriophage, and fungi. In a proof of concept experiment, next generation sequencing approaches, accompanied by the development of novel computational and bioinformatics tools, were utilized to examine the evolution of the microbial ecology of the avian trachea during the growth of a healthy commercial broiler flock. The flock was sampled weekly, beginning at placement and concluding at 49 days, the day before processing.
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- The study analyzed the diversity of Bacteria and Archaea in a degraded petroleum reservoir in Alaska using two culture-independent methods, showing no significant differences in diversity between the methods.
- Bacteria were classified into at least 14 phylum-level lineages, with Firmicutes making up 36.2% of identified rRNA gene clones, while a significant portion (35.2%) belonged to uncultured divisions.
- Archaea primarily consisted of methanogens, suggesting that acetate plays a crucial role in the microbial community as a key metabolite leading to methane production in this anaerobic environment.