Objective: Determine the dose of Vitamin C able to maintain a leukocyte Vitamin C concentration of > or =18 microg/10(8) cells, in pregnant women with 28 to 32 weeks of gestation, in order to compile a database to estimate the daily recommended intake (DRI) during pregnancy.
Methodology: Stage 1: acute supplementation study. A group of 10 healthy women in late first and early second trimester pregnancy were supplemented with 0 to 200 mg vitamin C/day during one week each.