Characterization of new non-ATP dependent inhibitors of TLK1 as potential molecules for treating prostate cancer.

Androgen deprivation therapy (ADT) is currently the primary treatment regime for Prostate cancer patients for advanced and local tumors. However, 70 % of the patients develop resistance to ADT due to various underlying mechanisms over the years. Researchers have identified the involvement of Tousled-like kinase 1 (TLK1) as a primary reason for ADT resistance and metastatic tumor development, representing TLK1 as an effective druggable target for prostate cancer.

Incidence and risk factors of acute transfusion reactions in patients admitted in intensive care unit on active monitoring after transfusion.

Background And Objectives: Transfusion reaction is often masked in critically ill patients because of underlying disease or certain pre-medications. The study was planned to know the incidence and determine risk factors associated with these acute transfusion reactions (ATRs) in intensive care (ICU) patients by actively monitoring the transfusion events.

Material And Methods: In this prospective observational study, critically ill patients admitted to ICU and having an ICU stay of 24 h prior to transfusion were enrolled.

PARP-16 regulates the PERK and IRE-1α Mediated Unfolded Protein Response in Japanese Encephalitis Virus-Infected Neural Stem/Progenitor Cells.

The viral infection and subsequent accumulation of viral proteins in the infected cells leads to endoplasmic reticulum (ER) stress. Japanese encephalitis virus (JEV) infection in the Central Nervous System (CNS) has been shown to induce unfolded protein response (UPR). The ER stress is resolved by the UPR which comprises certain signals that are transduced from the ER either to both the cytoplasm or nucleus, resulting in the adaptation for survival or may even lead to apoptosis.

The integrated stress response in neurodegenerative diseases.

The integrated stress response (ISR) is a conserved network in eukaryotic cells that mediates adaptive responses to diverse stressors. The ISR pathway ensures cell survival and homeostasis by regulating protein synthesis in response to internal or external stresses. In recent years, the ISR has emerged as an important regulator of the central nervous system (CNS) development, homeostasis and pathology.

Determining the function of ripening associated genes and biochemical changes during tomato (Solanum lycopersicum L.) fruit maturation.

This article examines biochemical alterations and gene expression changes during tomato fruit physiology. The chroma index increases from mature green (41.27) to red ripe (48.

Global transcriptome profiling of ST09 treated breast cancer cells identifies miR-197-5p/GPX3 antioxidant axis as a regulator of tumorigenesis.

ROS (Reactive Oxygen Species) has a dual role in tumorigenesis. Some cancers have high ROS conditions, and others have low ROS. TNBC thrives on high ROS compared to other Breast Cancer subtypes.

Stereomicroscopic Evaluation of Sealing Ability of Three Different Furcal Perforation Repair Materials: An Study.

Background: The choice of sealing material is a crucial factor that influences the outcome of endodontic treatment.

Aim: The purpose of this study was to compare the sealing ability of mineral trioxide aggregate (MTA) Angelus, Endocem MTA, and NeoPutty MTA when used as furcal perforation repair materials.

Materials And Methods: A total of 45 mandibular molars were used.

Trends in syphilis seroreactivity among blood donors: A 17-year retrospective analysis and follow-up at a tertiary care hospital.

Background And Objectives: This study aimed to assess the seroprevalence of syphilis among blood donors, evaluate the response rate of seroreactive donors, and investigate high-risk behaviors.

Material And Methods: The study presents a retrospective analysis of syphilis seroreactivity among blood donors over a 17 year period. Blood donations were screened for syphilis using the Rapid Plasma Reagin (RPR) card test.

Stereomicroscopic Evaluation of the Apical Sealing Ability of Different Root Canal Sealers (Endoseal, Apexit, MTA Fillapex, Ceraseal) Using Diaphanization Technique.

Introduction: Obturation of the root canal is the most critical step in endodontic treatment, which aims to provide a hermetic seal and prevent regrowth and entry of bacteria into the canal. To achieve this, many sealers are used in endodontics.

Aim: To evaluate the effectiveness of the apical seal obtained by different sealers used in conjunction with the single cone obturation technique using gutta-percha under the stereomicroscope.

In-silico evaluation of the T-cell based immune response against SARS-CoV-2 omicron variants.

During of COVID-19 pandemic, Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has continuously evolved, resulting in the emergence of several new variants of concerns (VOCs) with numerous mutations. These VOCs dominate in various regions due to increased transmissibility and antibody evasion, potentially reducing vaccine effectiveness. Nonetheless, it remains uncertain whether the recent SARS-CoV-2 VOCs have the ability to circumvent the T cell immunity elicited by either COVID-19 vaccination or natural infection.

Comparative Evaluation of the Effect of Various Storage Media on the Fracture Resistance of the Reattached Tooth Fragment: An Observational Study.

Background: Traumatic injuries are the most disruptive and distressing emergencies and pose a challenge for dental professionals because many different treatment protocols are currently available. They also affect the social and psychological well-being of children. A multidisciplinary approach is involved in the management of traumatic dental injuries to maintain function and esthetics.

Comparative Evaluation of Longevity of Fluoride Release from Three Different Fluoride Varnishes: An Observational Study.

Introduction: The cycles of demineralization and remineralization result in a dynamic process of caries development. Caries are prevented by the shift in the balance from demineralization to remineralization at the tooth-oral fluid interface with the help of salivary fluoride levels (in parts per million). The advantages of fluoride varnish application over other substitutes like dentifrices, mouthrinses, gels, or foams are that varnishes are well tolerated by infants, young children, or children with special healthcare needs and have prolonged therapeutic effects.

Disarib, a Specific BCL2 Inhibitor, Induces Apoptosis in Triple-Negative Breast Cancer Cells and Impedes Tumour Progression in Xenografts by Altering Mitochondria-Associated Processes.

Targeted cancer therapy aims to disrupt the functions of proteins that regulate cancer progression, mainly by using small molecule inhibitors (SMIs). SMIs exert their effect by modulating signalling pathways, organelle integrity, chromatin components, and several biosynthetic processes essential for cell division and survival. Antiapoptotic protein BCL2 is highly upregulated in many cancers compared with normal cells, making it an ideal target for cancer therapy.

Impact of telehealth on patient-provider communication in prenatal care for pregnant women from underserved settings.

Introduction: Telehealth has emerged as a promising supplementary modality in prenatal care. However, its impact on patient-provider communication (PPC), especially among pregnant women from underserved settings, requires comprehensive evaluation. This study examined the factors associated with the quality of patient-provider communication during the COVID-19 pandemic among pregnant telehealth users and non-users.

Azapeptides with unique covalent warheads as SARS-CoV-2 main protease inhibitors.

The main protease (M) of SARS-CoV-2, the causative agent of COVID-19, is a pivotal nonstructural protein critical for viral replication and pathogenesis. Its protease function relies on three active site pockets for substrate recognition and a catalytic cysteine for enzymatic activity. To develop potential SARS-CoV-2 antivirals, we successfully synthesized a diverse range of azapeptide inhibitors with various covalent warheads to target M's catalytic cysteine.

Antimicrobial effect of herbal and conventional root canal endodontic irrigants against persistent pathogens.

Evaluation and comparison of natural products like triphala, eucalyptus and carvacol with conventional root canal irrigant such as sodium hypochlorite (NaOCL) and Chlorhexidine against persistent root canal pathogens like is of interest. Samples were taken both before irrigation as well as after irrigation. CFU was counted after the plates had been incubated overnight at temperature of 37°C overnight.

Formulation of Functional Liquid Diets Under IDDSI Levels 0 to 2 for Dysphagia Patients: Physico-Chemical, Sensory, Antioxidant and Electromyographic Oral Processing Analyses.

Dysphagia is a significant health concern especially amongst the old age population. It is an ailment brought on by the weakening of the swallowing muscles. To reduce the risk of choking in dysphagia patients, the food is usually diluted to suit their swallowing ability.

Heterocyclic-Based Analogues against Sarcine-Ricin Loop RNA from : Molecular Docking Study and Machine Learning Classifiers.

Background: Heterocyclic-based drugs have strong bioactivities, are active pharmacophores, and are used to design several antibacterial drugs. Due to the diverse biodynamic properties of well-known heterocyclic cores, such as quinoline, indole, and its derivatives, they have a special place in the chemistry of nitrogen-containing heterocyclic molecules.

Objectives: The objective of this study is to analyze the interaction of several heterocyclic molecules using molecular docking and machine learning approaches to find out the possible antibacterial drugs.

Regulation of Onecut2 by miR-9-5p in Japanese encephalitis virus infected neural stem/progenitor cells.

Unlabelled: Japanese encephalitis virus (JEV) is one of the major neurotropic viral infections that is known to dysregulate the homeostasis of neural stem/progenitor cells (NSPCs) and depletes the stem cell pool. NSPCs are multipotent stem cell population of the central nervous system (CNS) which are known to play an important role in the repair of the CNS during insults/injury caused by several factors such as ischemia, neurological disorders, CNS infections, and so on. Viruses have evolved to utilize host factors for their own benefit and during JEV infection, host factors, including the non-coding RNAs such as miRNAs, are reported to be affected, thereby cellular processes regulated by the miRNAs exhibit perturbed functionality.

Delineating the mechanism of fragility at BCL6 breakpoint region associated with translocations in diffuse large B cell lymphoma.

BCL6 translocation is one of the most common chromosomal translocations in cancer and results in its enhanced expression in germinal center B cells. It involves the fusion of BCL6 with any of its twenty-six Ig and non-Ig translocation partners associated with diffuse large B cell lymphoma (DLBCL). Despite being discovered long back, the mechanism of BCL6 fragility is largely unknown.

Block Copolymer Encapsulation of Disarib, an Inhibitor of BCL2 for Improved Chemotherapeutic Potential.

A chemical inhibitor of antiapoptotic protein, BCL2, known as Disarib, suffers poor solubility in aqueous environments; thereby limiting its potential as a chemotherapeutic agent. To overcome this limitation and enhance the therapeutic efficacy of Disarib, we have employed the encapsulation of this small molecule inhibitor within P123 copolymer matrix. Micelles were synthesized using a thin-film hydration technique, and a comprehensive analysis was undertaken to evaluate the resulting micelle properties, including morphology, particle size, intermolecular interactions, encapsulation efficiency, and release characteristics.

Evaluation of potential role of R-loop and G-quadruplex DNA in the fragility of c-MYC during chromosomal translocation associated with Burkitt's lymphoma.

t(8;14) translocation is the hallmark of Burkitt's lymphoma and results in c-MYC deregulation. During the translocation, c-MYC gene on chromosome 8 gets juxtaposed to the Ig switch regions on chromosome 14. Although the promoter of c-MYC has been investigated for its mechanism of fragility, little is known about other c-MYC breakpoint regions.

Sodium Butyrate Induced Neural Stem/Progenitor Cell Death in an Experimental Model of Japanese Encephalitis.

The anti-inflammatory and neuroprotective effects of short chain fatty acid (SCFA) butyrate have been explored in a wide array of neurological pathologies. It is a 4-carbon SCFA produced from the fermentation of dietary fibers by the gut-microbiota. As evident from previous literature, butyrate plays a wide array of functions in CNS and interestingly enhances the differentiation potential of Neural stem/Progenitor Cells (NSPCs).