Optimizing Human Papillomavirus (HPV) Screening: Urine Sample Analysis and Associated Factors in Uzbekistan.

Objective This study assessed the accuracy of detecting Human Papillomavirus (HPV) DNA in urine samples compared to cervical samples and identified factors associated with HPV DNA positivity in Uzbekistan. Methods A total of 218 paired urine and cervical samples were collected from women in Uzbekistan. HPV DNA was detected using polymerase chain reaction (PCR) with genotyping.

[The comparative study of specificity of test-systems in diagnostic of HIV-infection on categories of samples of blood serum of pregnant women].

The detection of false positive serological reactions (FPSR) on HIV-infection under screening examination of pregnant women is an actual problem of practical health care. The original observations testify that under analysis of the same samples of blood serum of pregnant women using screening immune enzyme test-systems of various manufacturers the unmatched data concerning FPSR can be obtained. The purpose of this study was to implement comparative evaluation of specificity of immune enzyme test-systems of three different manufacturers: "DS-IFA-HIV-AGAT-SCREEN" ("Diagnostic Systems"), "Genscreen Ultra HIV Ag-Ab" "Bio Rad" France) and "The CombiBest HIV-1,2 AG/AT" ("Vector-Best" Novosibirsk).

Biomimetic cell-mediated three-dimensional assembly of halloysite nanotubes.

Biomimetic architectural assembly of clay nanotube shells on yeast cells was demonstrated producing viable artificial hybrid inorganic-cellular structures (armoured cells). These modified cells were preserved for one generation resulting in the intact second generation of cells with delayed germination.

A whole-cell amperometric herbicide biosensor based on magnetically functionalised microalgae and screen-printed electrodes.

We report the fabrication of an amperometric whole-cell herbicide biosensor based on magnetic retention of living cells functionalised with magnetic nanoparticles (MNPs) on the surface of a screen-printed electrode. We demonstrate that Chlorella pyrenoidosa microalgae cells coated with biocompatible MNPs and retained on the electrode with a permanent magnet act as a sensing element for the fast detection of herbicides. The magnetic functionalisation does not affect the viability and photosynthesis activity-mediated triazine herbicide recognition in microalgae.

[Use of pH-sensitive immobilized monoclonal antibodies for optimization of immunoenzyme sandwich technique of detection of HBsAg of hepatitis B virus].

Aim: To develop highly sensitive sandwich technique for identification of surface hepatitis B virus antigen (HBsAg) in serum and analyse of possible improvement of solid phase for immunoenzyme sandwich technique of HBsAg identification through variation of pH-dependent sorption of monoclonal antibodies on the surface of immune plates.

Materials And Methods: Calibration curves for identification of HBsAg in sandwich techniques using 36 possible binary combinations of monoclonal antibodies of our panel (including high affinity antibodies to HBsAg produced by 6 hybridomas) were compared. Immobilization of antibodies on solid phase (by passive sorption) was performed at different pH values (2.

Polyelectrolyte-mediated assembly of multiwalled carbon nanotubes on living yeast cells.

Here we report the three-dimensional assembly of carbon nanotubes on the polyelectrolyte-coated living Saccharomyces cerevisiae cells using the polyelectrolyte-mediated layer-by-layer approach. Synthetic polyelectrolytes poly(allylamine hydrochloride) and poly(sodium 4-styrenesulfonate) were layer-by-layer deposited on the surfaces of the yeast cells followed by the deposition of water-soluble oxidized multiwalled carbon nanotubes (MWNTs) and an additional outermost polyelectrolyte bilayer. This resulted in the fabrication of polyelectrolyte/nanotubes composite coatings on the cell walls of the yeast cells, which could be clearly seen using the conventional optical microscopy.

[The capacities of current test systems to verify early HIV infection].

The purpose of the present investigation was to comparatively evaluate the performance characteristics of the test systems designed to verify the positive results of screening survey for HIV infection, such as the solid-phase immunoassay DS-EIA-HIV-AB/AG-SPECTR (Diagnosticheskiye Sistemy (Diagnostic Systems) Research-and-Production Association, Nizhni Novgorod) and tests based on immune blotting (IB). The investigation examined 15 seroconversion panels produced by ZeptoMetrix (USA) and BBI (USA). The use of the DS-EIA-HIV-AB/AG-SPECTR test system determined 88 of the 167 seroconversion panels as HIV positive.

[Diagnostic advantages of the test system "DS-EIA-HBsAg-0.01" for detection of HBV surface antigen].

The new highly sensitive test system "DS-EIA-HBsAg-0.01" (Priority Certificate No. 2006129019 of August 10, 2006) in detecting hepatitis B surface antigen (HBsAg) was assessed.

[A novel enzyme immunoassay system for the detection of HIV-1 p24 antigen with a sensitivity of 0.5 pg/ml].

A "DS-EIA-HIV-AG-Screen" enzyme immunoassay system has been devised to detect HIV-1 p24 antigen with a sensitivity of 0.5 pg/ml the use of which permits reduction of a seronegative window phase as compared with the tests showing a lower sensitivity threshold. The "DS-EIA-HIV-AG-Screen" system developed may be used to screen donor blood and to examine risk-group individuals for the early diagnosis of HIV infection.

[Development and assessment of a new test for verification of tests for HIV-infection markers].

The OOO "Research-and-Production Association "Diagnostic Systems" has developed a "DC-EIA-HIV-AT/AG-SPECTRUM" screening enzyme immunoassay system designed to detect separately antibodies to certain HIV-1 and HIV-2 proteins, as well as antigen p24. The determination of antibodies of all classes and the marker of early-stage infection antigen p24 with a high (5 pg/ml) sensitivity substantially reduces the number of void results obtained in the use of immunoblots. The developed "DC-EIA-HIV-AT/AG-SPECTRUM" plate system is an effective tool to support positive screening results and may be used at the final stage of laboratory diagnosis of HIV infection.

[Evaluation of diagnostic efficiency of the recombinant protein modeling immunodominant epitope V3 of envelope gp120 for immunoenzyme detection for HIV-1 infection antibodies].

The third hypervariable domain V3 of the human immunodeficiency virus type 1 gpl20 envelope glycoprotein contains neutralizing epitopes and plays an important role in the diagnosis of HIV infection . Neutralizing antibodies bind to conserved epitope with sequence GPG of V3 loop. The effect of sequence variation on the antigenic properties of the V3 epitope gp120 was studied using five synthetic peptides.

[Comparative study of the reactivity, safety and immunogenicity of "Havrix" inactivated hepatitis A vaccine].

This paper sums up the results of controlled field clinical trials of adult and childhood variants of Harvix, inactivated vaccine from hepatitis A. The vaccine is weakly reactogenic and safe. After a single injection of Harvix-1440 vaccine to adults the percentage of seroconversions in previously seronegative individuals was 80.

[The effectiveness of using the Vaxigrip vaccine from the firm of Pasteur Mérieux Connaught for influenza prevention in organized groups of adults].

The results of the study of the effectiveness of using vaccine Vaxigrip for the prophylaxis of influenza in organized groups of adults are presented. The vaccine was found to have high epidemiological effectiveness (the epidemiological index was 2.6), moderate reactogenicity and pronounced immunological activity (the protection level was 89.

[An evaluation of the epidemiological and economic efficacy of immunizing adults with the Vaxigrippe vaccine].

The results of the evaluation of the epidemiological and economic efficiency of the immunization of the adult population with the vaccine Vaxigrip (Pasteur Mérieux Connaught, France) are presented. In accordance with the results obtained in this investigation, this vaccine was found to have pronounced epidemiological (prophylactic) efficiency and to be well tolerated. To achieve a perceptible epidemiological effect, it was sufficient to cover 43% of the group with vaccination.

[An assessment of the connection between the annual population morbidity of salmonellosis due to Salmonella enteritidis and the dynamics of the epizootic process among chickens in commercial poultry plants].

The dynamics of annual morbidity in salmonellosis caused by S. enteritidis among the population of Perm during the period of 1987-1992 was analyzed. Blood sera taken from 4,689 practically healthy donors and from 6,997 hens at poultry breeding complexes were studied in the passive hemagglutination test with the use of complex Salmonella diagnosticum.