[Clinical and pathogenetic significance of collagen metabolism disorder in children with gastroesophageal reflux disease].

In 62 children with gastroesophageal reflux disease (GERD) and 32 with gastroduodenitis (DG) aged 9-17 years, the peculiarities of metabolism of collagen were studied. High levels of fractions of sialic acids were set, that was associated with the protein fructose, fractions of hydroxyproline in children with GERD compared with the patients with DG, which testify to the process of degradation of collagen and may be one of the factors contributing to the local inflammation of the esophagus and gastroduodenal zone of the digestive tract. The prevalence of Helicobacter pylori, as well as violations of diet, play an important role in maintaining the inflammatory process.

[A procedure for determination of acid mannosidase activities in biological fluids].

A procedure has been developed for simultaneous determination of the activities of alpha-D- and beta-D-mannosidase in the biological fluids from the quantity of free 4-nitrophenol. The latter is released via enzymatic degradation of substrates of 4-nitrophenyl-alpha-D-mannose and 4-nitrophenyl-beta-D-mannose in individual incubation tests.

[A technique for determination of free and peptide-bound hydroxyproline in blood serum].

A technique has been developed to determine free and peptide-bound hydroxyproline in blood serum. It is based on the principle of chloramine oxidation of hydroxyproline and condensation of oxidation products with p-dimethylaminobenzaldehyde. Data on changes in the serum level of hydroxyproline in children with acute pneumonia and in pregnant women are presented.

[Age-related changes in the exchange of hexosamine-containing biopolymers in rats under immobilization stress].

Daily double spinal immobilizations (for 60 minutes, at intervals of 7-8 hours) for 3 days were studied for their impact on the metabolism of hexosamine-containing biopolymers in the mucosa of the stomach and lung in male rats of different ages (kids, adults, and olds). At the same time, the highest increases in the level of hexosamine glycane, glycoprotein, and hexosamine-containing glycopeptides and a rise in the activity of the lysosomal enzymes N-acetyl-beta-D-hexosaminidase (EC 3.2.

[Detection of serum fucosidase activity].

A procedure has been developed for simultaneous determination of the activity of a-L-fucosidase (EC 3.2.1.

[Metabolism of the main substance of the connective tissue in chronic obstructive pulmonary disease].

Aim: To reveal metabolic disturbances of connective tissue main substance in chronic obstructive pulmonary disease (COPD) and to assess their informative value in determination of treatment efficacy.

Material And Methods: Peripheral blood of 130 COPD patients was studied for total glycosaminoglycanes, hyaluronidase activity, sialic acids fractions (free, oligobound and proteinbound), fucose fractions (oligobound and proteinbound) and activity of sialidase.

Results: The levels of total glycosaminoglycanes, sialo- and fucose-containing compounds in the blood change with COPD severity and phase of the disease.

[Determination of serum fucosidase activities].

A method has been developed for simultaneous determination of the activity of alpha-L-fucosidase (EC 3.2.1.

[Characteristics of collagen metabolism in duodenal ulcer].

We studied the content of collagen metabolites in patients with duodenal ulcer (blood plasma, urea, and samples of the duodenal mucous tunic and mouth fluid). We also defined the role of an affected collagen metabolism in the formation and cicatrization of duodenal ulcer; finally, we ascertained that the nature of the detected changes in different biological substrate is unidirectional.

[Detection of N-acetyl-beta-D-hexosaminidase in urine].

A new modification was designed to determine the activity of N-acetyl-beta-D-hexosaminidase (NAH) in biological fluids. The properties of enzyme molecules to adsorb themselves on chitin at pH 4.5 were made use of within the case study.

[Analysis of protein- and oligomer-bound fucose in blood serum].

A new method was suggested that provides for a simultaneous determination of protein- and oligomer-bound fucose in blood serum. The level of such compounds in blood serum was shown to be increasing with age and in certain diseases.

[The method for detection of urinary lysozyme].

A method for lysozyme measurement in the urine is developed, based on adsorption of lysozyme molecules on the surface of chitin. The urine of healthy children, men, and women contains 1.76, 1.

[Status of sialo- and fuco-containing compounds in chronic gastroduodenitis in children].

In exacerbation of chronic gastroduodenitis in children an increase in the content of decay products of glycoprotein components and a decrease of protein-bound fucosa were recorded in the basal and stimulated portions of gastric juice. The change of mucous glycoprotein properties and, therefore, mucous protective barrier of the stomach are important factors in pathogenesis of chronic gastroduodenitis exacerbation in children.

[Method of measuring non-protein bound fucose].

A method for measuring fucose not bound to proteins in the serum is developed. The level of this enzyme is shown to increase with age. It is increased in adult patients with myeloma, liver cirrhosis, and rheumatic fever and in children with exacerbations of chronic osteomyelitis and gastroduodenitis.

[Periodontal function after replantation].

The components of periodontal connective tissue were studied on days 28-30 after replantation of the teeth with the extra-alveolar time 5, 15, and 30 min. The levels of collagen, glucosaminoglycanes, sialic acids, and activity of sialidase increased at extra-alveolar time 30 min by 44, 33, and 20, and 85%, respectively. Introduction of potassium hyaluronate in dental wells reduced the sail collagen growth in the periodontal tissue.

[The determination of sialidase activity in the blood serum and saliva].

A method for sialidase (neuraminidase; EC 3.2.1.

[Determination of free and bound forms of sialic acids in biologic objects].

A method for simultaneous measurements of free, oligomer-bound and protein-bound sialic acids in the blood serum, saliva, and tissue homogenates is offered. The results of these parameters measurements after stressogenic exposures and in some diseases are presented.

[The effect of vicasol and pelentan on the biopolymers of the periodontium].

Vitamin K (vikasol) and pelentan were examined for their effects of periodontal tissue levels of collagen, hexosamine-containing biopolymers and sialoglycoproteins. During tooth replantation in dogs, vitamin K was demonstrated to elevate the levels of collagen, hexosamine-containing polymers in periodontal tissue by 25.8, 19.

[Change in indicators of biopolymer metabolism in connective tissue upon central administration of angiotensin II].

Some patterns of connective tissue metabolism were studied in rabbit blood and aorta after local administration of angiotensin II into lateral ventricle of brain and ventromedian nucleus of hypothalamus. Within the first 10 days of the chronic experiments catabolism of connective tissue predominated, while anabolic processes, accompanied by accumulation of collagen in aorta, were elevated within 30 days. The alterations observed were most distinct after microinjection of angiotensin II into hypothalamus.

[Indices of hexosamine-containing biopolymers in experimental tooth replantation].

The experimental findings showed that the content of hexosamine-containing biopolymers, in periodontal tissue increased by 38.23 and 124% conformably over 14 days after replantation of 4 teeth of the dogs. The indices normalized in 28 days of the experiment.