Isolation, identification, antibiotic resistance profile and molecular analysis of Ornithobacterium rhinotracheal isolates from turkeys.

Background: Ornithobacterium rhinotracheal (ORT) infects numerous birds, particularly chickens and turkeys. ORT is an emerging bacterial pathogen of global concern in the poultry industry. As ORT is rapidly spreading throughout commercial poultry, it requires intensive studies of its epidemiology, diagnostic procedures, molecular typing, virulence genes and antimicrobial resistance.

The effects of omega-3 fatty acids supplementation on hemoglobin, hematocrit, and platelet levels of patients with ESRD condition undergoing dialysis.

Background: Concomitant inflammation may boost the cardiovascular complications in end-stage renal disease (ESRD) patients undergoing hemodialysis (HD). Omega-3 fatty acids may have certain health benefits in HD patients. The aim of this study was to investigate the effects of omega-3 fatty acids supplementation on hematocrit (HCT), hemoglobin (HB) level and platelet (PLT) counts of HD patients.

Does dietary intake of caffeine have an effect on transient global amnesia?

Aim: Amnesia is a cognitive disorder that may lead to memory loss. Caffeine is a psychoactive substance which have an effect on memory and cognitive functions. This study aimed to assess the association of transient global amnesia (TGA) with dietary intake of caffeine.

Association between type 2 diabetes and different types of dietary fats: A case-control study.

Background: The effect of dietary fats on type 2 diabetes (T2D) is not clear. This study aimed to determine the association between T2D and dietary fatty acids among Iranian adults.

Methods: This case-control study was performed on 4241 participants aged 35-70, including 1804 people with T2D and pre-diabetes as the case group and 2437 people without diabetes as the control group.

Method development for simultaneous determination of active ingredients in cough and cold pharmaceuticals by high performance liquid chromatography.

A rapid, simple and efficient liquid chromatographic method was developed for simultaneous determination of three active ingredients namely, chlorpheniramine maleate, phenylephrine hydrochloride and guaifenesin along with sodium benzoate preservative common cold medications (syrups) and the method was validated based on the International Conference on Harmonization (ICH) and United State Pharmacopeia (USP) guidelines. Separation of the analytes was achieved within 15 min on a nucleosil gravity phenyl column in a single run with a mobile phase consisting of methanol: buffer mixture (15:85 v/v) at room temperature, in isocratic mode with flow rate of 0.8 mL min.

Methicillin resistance and selective genetic determinants of isolates with bovine mastitis milk origin.

Background And Objectives: is one of the major causes of bovine mastitis, which can be transmitted from animals to humans. Methicillin-resistant (MRSA) isolates are more attentive and if not treated promptly, they can cause death. The aim of this study was to determine the prevalence of methicillin resistance and frequency of selected virulence factors of isolates with bovine mastitis milk origin in Ahvaz, southwest of Iran.

Protective and immunogenic effects of Escherichia coli-expressed infectious pancreatic necrosis virus (IPNV) VP2-VP3 fusion protein in rainbow trout.

Infectious Pancreatic Necrosis Virus (IPNV) is a member of the family Birnaviridae which causes significant losses in the aquaculture industry. To develop a recombinant vaccine for IPNV, a cDNA construct of IPNV VP2-VP3 fusion gene was prepared and cloned into an Escherichia coli (E. coli) expression vector (pET-26b) to obtain recombinant protein products.

Antigenic detection of group A in diarrheic dogs in Ahvaz district, Southwestern Iran.

The aim of the present survey was to evaluate the prevalence of (CRV) infection in companion dogs in Ahvaz district, Iran. The studied dogs were selected between referred cases to Shahid Chamran University of Ahvaz. Fecal samples were collected from 98 diarrheic dogs between December 2011 and August 2013.

Epitope mapping of bovine viral diarrhea virus nonstructural protein 3.

Six consecutive overlapped coding regions (F1-F6) of whole NS3 molecule of bovine viral diarrhea virus (BVDV) were cloned into pMAL-c2X plasmid vector and expressed in Escherichia coli cells (BL21 strain). The recombinant proteins were then purified by amylose resin to determine the most immunogenic domain(s) of the NS3 molecule. Evaluation of the recombinant proteins was carried out by indirect ELISAs using several bovine sera (previously characterized by virus neutralization test, a commercial ELISA kit, and a newly developed NS3-ELISA) and 6 monoclonal antibodies.

Determination of Giardia duodenalis genotypes in sheep and goat from Iran.

Giardia duodenalis is an important zoonotic intestinal protozoan worldwide So far, seven assemblages have recognized for G. duodenalis (A-G) and there are the firm findings which assemblages A and B have zoonotic potential and assemblage E in livestock. In the presented work, the G.

Solid phase extraction of trace amounts of silver, cadmium, copper, mercury, and lead in various food samples based on ethylene glycol bis-mercaptoacetate modified 3-(trimethoxysilyl)-1-propanethiol coated Fe3O4 nanoparticles.

Extraction, pre-concentration, and determination of trace amounts of silver, cadmium, copper, mercury, and lead from some food samples were investigated by magnetic solid phase extraction using Fe3O4 nanoparticles coated with 3-(trimethoxysilyl)-1-propanethiol and modified with ethylene glycol bis-mercaptoacetate as a new adsorbent. SEM, X-ray diffraction, and FT-IR were used to characterise the adsorbent. Metal ions were measured using ICP-OES, except for mercury, which was determined by CV-AAS method.

Extraction and Determination of Cyproheptadine in Human Urine by DLLME-HPLC Method.

Novel dispersive liquid-liquid microextraction (DLLME), coupled with high performance liquid chromatography with photodiode array detection (HPLC-DAD) has been applied for the extraction and determination of cyproheptadine (CPH), an antihistamine, in human urine samples. In this method, 0.6 mL of acetonitrile (disperser solvent) containing 30 μL of carbon tetrachloride (extraction solvent) was rapidly injected by a syringe into 5 mL urine sample.

Sequence analysis of infectious pancreatic necrosis virus isolated from Iranian reared rainbow trout (Oncorhynchus mykiss) in 2012.

Infectious pancreatic necrosis virus (IPNV) is the causal agent of a highly contagious disease that affects many species of fish and shellfish. This virus causes economically significant diseases of farmed rainbow trout, Oncorhynchus mykiss (Walbaum), in Iran, which is often associated with the transmission of pathogens from European resources. In this study, moribund rainbow trout fry samples were collected during an outbreak of IPNV in three different fish farms in north and west provinces of Iran in 2012; and we investigated the full genome sequence of Iranian IPNV and compared it with previously identified IPNV sequences.

Characterization of Eimeria Species in Commercial Broilers by PCR Based on ITS1 Regions of rDNA.

Background: Coccidiosis is an intestinal disease of chickens caused by various species of protozoan parasites within the genus Eimeria. Diagnosis and genetic characterization of different species of Eimeria are central to the prevention, surveillance, and control of coccidiosis. The aim of this study was to detect different chicken Eimeria species from several areas in Khuzestan, southwest Iran.

Antibody titers against canine distemper virus in unvaccinated rural dogs from Ahvaz, Iran.

The purpose of this study was to evaluate the prevalence of antibodies to Canine Distemper Virus (CDV) in unvaccinated rural dogs without known immunization status and to assess risk factors for infection by means of indirect immunofluorescent assay (IFA), in Ahvaz district, southwest of Iran. Serum samples were randomly collected from 97 healthy dogs 6 months and older from villages around Ahvaz city between 2004 and 2005. Dogs were grouped by age and sex to determine whether these factors were associated with antibody titers, using Fisher's exact test.

Molecular G typing of bovine rotaviruses in Iran.

Fifty rotavirus-positive feacal samples, selected from 500 ELISA tested diarrheic specimens were used in this study. Viral RNA was extracted from each sample and reveres transcribed to cDNA. The cDNA was then amplified by oligonucleotide primers specific for RNA segment 9, coding for VP7.

Immunogenicity of E. coli-expressed sigma3 protein of avian reovirus in chickens.

An E. coli-expressed fusion protein was used to study the immunogenicity of avian reovirus sigma3 protein in chickens. The protein induced the production of specific antibodies detectable by immunofluorescence, ELISA and immunoblot.

Evidence for the multimeric nature and cell binding ability of avian reovirus sigma 3 protein.

It has been suggested that avian reovirus sigma 3 protein is analogous to sigma 1 trimer, the mammalian reovirus attachment protein. We have investigated the multimeric nature and cell binding ability of sigma 3 protein. The data presented here demonstrate that sigma 3 protein is a multimer in its undisrupted form as determined by SDS-PAGE in non-dissociating conditions.

Characterization of monoclonal antibodies against avian reovirus strain S1133.

Monoclonal antibodies (MAbs) were produced against a vaccinal S1133 strain of avian reovirus. Characterization of six MAbs in Western blotting, radioimmunoprecipitation and gold immunoelectron microscopy revealed that the MAbs were specific to the outer capsid proteins, mu2/mu2c, sigma2 and sigma3. Two of three MAbs, directed against sigma2 protein, neutralized the virus infectivity in a broadly specific manner, whereas the third had no neutralizing activity.

Cloning, sequencing and expression of the S1 gene of avian reovirus.

The S1 genome segment of avian reovirus strain S1133 was cloned and completely sequenced. The sequence comprised 1636 bp with three distinct open reading frames (ORFs), suggesting the gene was polycistronic in nature. The three ORFs from 5' to 3' were predicted to encode polypeptides of 9.

Interaction of avian reovirus with chicken lymphoblastoid cell lines.

Four chicken lymphoblastoid cell lines were inoculated with avian reovirus strain S1133 and two local isolates, 965 and 615. Of the inoculated cell lines, TLT, a B-cell line, was productively infected with the three viruses as demonstrated by immunofluorescence assay (IFA) and radioimmunoprecipitation assay. A comparative growth curve analysis of the three avian reoviruses was done at 37 degrees and 41 degrees C.