Characterization and Developmental Expression Patterns of Four Hexamerin Genes in the Bumble Bee, Bombus terrestris (Hymenoptera: Apidae).

Hexamerins are members of the hemocyanin superfamily and play essential roles in providing amino acids and energy for the nonfeeding stages of insects. In this study, we cloned and analyzed the expression patterns of four hexamerin genes (hex 70a, hex 70b, hex 70c, and hex 110) at different worker development stages and queen diapause statuses in the bumble bee, Bombus terrestris. The results of this study showed that hex 110 has the longest open reading frame (ORF; 3,297 bp) compared to the ORFs of hex 70a (2,034 bp), hex 70b (2,067 bp), and hex 70c (2,055 bp).

Assessment of the value of ecosystem services in water sources of the South-North Water Diversion Central Project: the case of Dengzhou City, Henan Province.

Dengzhou City is the first ecological water recharge city after the commissioning of the South-North Water Diversion Project. A study on the ecological service value of Dengzhou City and its evolutionary characteristics can provide a theoretical basis for the water supply security of the Central Line Project and regional ecological environmental protection. Based on the ecosystem service value equivalent factor method, GIS, ENVI and GeoSOS-FLUS software were used to monetize the ecosystem service values of Dengzhou City in 2007, 2011, 2014, 2015 and 2020; analyze the spatial and temporal change characteristics under its land use change conditions and predict the regional ecosystem service values of Dengzhou City in 2025.

Evaluation of wetland ecosystem services value of the yellow river delta.

Analyzing and evaluating the ecosystem service value of wetlands can provide technical support and theoretical basis for regional socio-economic development, ecological environmental protection, and other planning measures. Based on the factors of hydrology, meteorology, society, economy, resources, environment, and culture, the wetland ecosystem services value of Yellow River Delta (YRD) was evaluated by the method of functional value and equivalent factor, which has certain reference significance for future research. Two methods were used to calculate the ecosystem services value of YRD in 2016, and the results were $3.

IRP30 promotes worker egg-laying in bumblebee, Bombus terrestris (Hymenoptera: Apidae).

Bumblebees are important pollinators that have evolved between solitary and advanced eusocial insects. Compared with advanced honeybees, workers of social bumblebee species are prone to laying eggs during the competition phase, which leads to the end of the colony. Therefore, worker reproductive behavior has become a popular research topic for exploring various biological phenomena.

Characterization of a Vitellogenin Receptor in the Bumblebee, (Hymenoptera, Apidae).

The vitellogenin receptor (VgR) belongs to the low-density lipoprotein receptor (LDLR) family, responsible for mediating the endocytosis of vitellogenin (Vg) into the ovaries to promote ovarian growth and oviposition. Here, we cloned and measured gene expression characteristics in the bumblebee . RNA interference was used to validate VgR function.

microRNA-126 inhibits tube formation of HUVECs by interacting with EGFL7 and down-regulating PI3K/AKT signaling pathway.

It's critical for tube formation and angiogenesis to repair ischemic myocardium or stroke. This study aimed to investigate role of microRNA-126 (miR-126) in tube formation in human umbilical vein endothelial cells (HUVECs) and associated mechanisms. Primary neural stem cells (NSCs) and HUVECs were cultured and transfected with microRNA-126 mimics and miR-126 inhibitor.

The complete mitochondrial genome of wild honeybee (Hymenoptera: Apidae) in south-western China.

We sequenced almost complete mitochondrial genome of (Insecta: Hymenoptera: Apocrita: Apidae) with length of 15,933 bp. The genome has similar codon usage and gene organization to those of mitogenome reported for other Hymenoptera. It includes 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes and a noncoding region with very high AT-base content.

mRNA expression and DNA methylation in three key genes involved in caste differentiation in female honeybees (Apis mellifera).

In honeybee (Apis mellifera) colonies, queens and workers are alternative forms of the adult female honeybee that develop from genetically identical zygotes but that depend on differential nourishment. Queens and workers display distinct morphologies, anatomies and behavior, better known as caste differentiation. Despite some basic insights, the exact mechanism responsible for this phenomenon, especially at the molecular level, remains unclear although some progress has been achieved.

Quantification of major royal jelly protein 1 in fresh royal jelly by indirect enzyme-linked immunosorbent assay.

Rabbit polyclonal antibody produced by a major royal jelly protein 1 (MRJP1) specific peptide reacted only with a MRJP1. Indirect ELISA with the antibody revealed a MRJP1 level of 4.12-4.

[Phylogenetic relationships of honey bees (Hymenoptera: Apinae) inferred from mitochondrial and nuclear DNA sequences].

The sequences of mitochondrial ND2, CO2, and 16S rRNA genes and nuclear ITPR gene were obtained from 22 samples of 5 Apis species from China. The characteristics of the sequences and the pairwise distances among species were analyzed. Phylogenetic trees were reconstructed for Apis species using maximum parsimony, neighbor-joining and maximum likelihood methods together with the sequences of the other 4 Apis species downloaded from GenBank.

Role of procalcitonin in the diagnosis of severe infection in pediatric patients with fever and Neutropenia--a systemic review and meta-analysis.

Objective: The aim of this study was to determine the accuracy of the procalcitonin (PCT) test for diagnosis of bacterial sepsis in pediatric cancer patients with febrile neutropenia.

Methods: Three major databases, MEDLINE, EMBASE and the Cochrane Library were searched for studies that evaluated the diagnostic value of PCT alone or compared with other laboratory markers such as C-reactive protein (CRP) to identify bacterial sepsis in children with fever and neutropenia. A bivariate model was used to derive summary sensitivity and specificity of the diagnostic tests.

A method of extracting ginsenosides from Panax ginseng by pulsed electric field.

The objective of this study was to develop and optimize a pulsed electric field (PEF) extraction method. Various experimental conditions, including electric field intensity and frequency, were evaluated against extraction methods. The content of six major ginsenosides (Rg(1), Re, Rb(1), Rc, Rb(2), and Rd) were quantified by HPLC.

Gigantism in honeybees: Apis cerana queens reared in mixed-species colonies.

The development of animals depends on both genetic and environmental effects to a varying extent. Their relative influences can be evaluated in the social insects by raising the intracolonial diversity to an extreme in nests consisting of workers from more than one species. In this study, we studied the effects of mixed honeybee colonies of Apis mellifera and Apis cerana on the rearing of grafted queen larvae of A.