Publications by authors named "Shaoquan Shi"

Background: Previously, we have demonstrated that the batch variations of human platelet lysate (conventional MSC expansion medium) induce MSC heterogeneity and therapeutic inconsistency. On the other hand, the MSCs expanded with chemical defined medium have improved therapeutic consistency.

Methods: In the current study, we studied the MSC subpopulation composition and variation in different types and batches of MSC expansion medium with scRNA-seq analysis.

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Background: Although both preclinical and clinical studies have shown the great application potential of MSCs (mesenchymal stem/stromal cells) in treating many kinds of diseases, therapeutic inconsistency resulting from cell heterogeneity is the major stumbling block to their clinical applications. Cell population diversity and batch variation in the cell expansion medium are two major inducers of MSC heterogeneity.

Methods: Cell population diversity was investigated through single-cell RNA sequencing analysis of human MSCs derived from the umbilical cord and expanded with fully chemically defined medium in the current study.

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Objective: To compare the effects of letrozole and human menopausal gonadotropin (HMG) in the treatment of patients with polycystic ovary syndrome (PCOS) resistant to clomiphene citrate (CC).

Methods: A total of 96 clomiphene resistance polycystic ovary syndrome patients infertility were randomly divided into an LE group, and HMG group (n = 48). LE group orally received letrozole at 5.

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Article Synopsis
  • - The study aimed to evaluate the use of comparative genomic hybridization (array-CGH) for diagnosing mandibulofacial dysostosis in a fetus identified through prenatal ultrasound.
  • - The research involved collecting amniotic fluid and blood samples, with conventional cytogenetic analysis showing no abnormalities, but array-CGH revealing a duplication in a specific gene region (1p36.33) linked to cartilage development.
  • - The identification of the genes VWA1 and PYGO2 through array-CGH was confirmed with RT-qPCR, ultimately leading to a postnatal diagnosis of mandibulofacial dysostosis.
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