Analysis of Flow Field Characteristics in a Mechanical Defoaming Device.

To improve the froth breakdown performance of flotation foam, a mechanical defoaming device based on vacuum and rotation was developed. The device is mainly composed of a rotating disk and a baffle and generates negative pressure when rotating at high speed. Through the synergistic effects of negative pressure, collision, extrusion, and shearing, the flotation foam effectively separates the liquid and gas phases.

Effect of Surfactants on Eliminating Stable Ultrafine Chalcopyrite Froth.

Chalcopyrite is a primary source of copper in nature. However, with the increasing need to process low-grade and complex chalcopyrite ores, overly stable froth is becoming more and more common and poses operational and safety challenges. No reliable strategy has been developed to address the issue.

A sensitive "off-on" carbon dots-Ag nanoparticles fluorescent probe for cysteamine detection via the inner filter effect.

In this study, we describe the construction of an "off-on" fluorescent probe based on carbon dots (CDs) and silver nanoparticles (AgNPs) mixture for sensitive and selective detection of cysteamine. By mixing AgNPs with CDs solution, the fluorescence of CDs was significantly decreased due to the inner filter effect (IFE). Upon addition of cysteamine to the mixed aqueous of CDs and AgNPs, the silver-sulfur bond between cysteamine and AgNPs caused AgNPs to aggregate, and the quenched fluorescence of CDs could in turn be recovered.

A novel fluorescent probe for the localization of nucleoli developed via a chain reaction of endogenous cysteine in cells.

Nucleolus imaging is important for the understanding of gene expression, proliferation, and growth of cells. Traditional nucleoli localization mainly relies on the use of RNA fluorescent probes which are required in large amounts. These probes also have low selectivity, thus causing the generated images to have high background noise and the localization of nucleoli to become vague.

Lysosome-targeted ratiometric fluorescent sensor for monitoring pH in living cells based on one-pot-synthesized carbon dots.

A hydrothermal method has been employed to synthesize a green and one-pot carbon dots-based sensor for ratiometric monitoring and imaging lysosomal pH in living cells. The carbon dots were directly functionalized by abundant amino groups during synthesis and exhibited dual emission bands at 439 and 550 nm under single-wavelength excitation of 380 nm without any additional modification. In addition to its small size, the established sensor had good biocompatibility.

One-step fabrication of boronic-acid-functionalized carbon dots for the detection of sialic acid.

Typically, sialic acids (SA) with a nine-carbon backbone are found at the glycan chain termini on the cell membranes, which play crucial roles in various physiological and pathological processes. The expression level of SA in the blood serum has been reported to correlate with various disease states among cancer. In this study, a novel approach for preparing fluorescent boronic-acid-modified carbon dots (C-dots) for the detection of SA was developed.

Selective and sensitive fluorescence detection method for pig IgG based on competitive immunosensing strategy and magnetic bioseparation.

A novel fluorescence detection method based on competitive immunoassay and magnetic bioseparation technique was developed and applied to the determination of pig immunoglobulin G (IgG) in serum samples. Core-shell structured FeO@SiO nanoparticles were synthesized by chemical coprecipitation, followed by functionalization with amino groups and immobilization of pig IgG antibodies. The synthesized FeO@SiO-antibody nanoparticles were employed as the probe for the competitive immune recognition of the target antigens in samples and the antigens labeled with fluorescein isothiocyanate (FITC).

Disruption of ku70 involved in non-homologous end-joining facilitates homologous recombination but increases temperature sensitivity in the phytopathogenic fungus Penicillium digitatum.

The dominant mechanism to repair double-stranded DNA breaks in filamentous fungi is the non-homologous end joining (NHEJ) pathway, and not the homologous recombination (HR) pathway that operates in the mutation of genes by replacement of target DNA for selection cassettes. The key to improve HR frequency is the inactivation of the NHEJ pathway by eliminating components of its Ku70/80 heterodimeric complex. We have obtained ku70 mutants of Penicillium digitatum, the main citrus postharvest pathogen.