Schisandrin B Inhibits LPS-Induced Endometritis Through Attenuating Ferroptosis via AMPK/PGC1α/Nrf2 Signalling Pathway.

Endometritis is one of the common reproductive diseases in human and animal. In recent years, a number of studies have found that Schisandra B (Sch B), as a natural Chinese medicine extract, has antioxidant, anti-inflammatory and other biological activities. Based on the above, in this study, mice were used to conduct an in vivo experiment to investigate the effect and mechanism of Sch B on lipopolysaccharide (LPS)-induced endometritis.

Targeting HSP90 in Gynecologic Cancer: Molecular Mechanisms and Therapeutic Approaches.

One of the leading causes of mortality for women is gynecologic cancer (GC). Numerous molecules (tumor suppressor genes or oncogenes) are involved in this form of cancer's invasion, metastasis, tumorigenic process, and therapy resistance. Currently, there is a shortage of efficient methods to eliminate these diseases, hence it is crucial to carry out more extensive studies on GCs.

Cognitive impairment in Chinese traumatic brain injury patients: from challenge to future perspectives.

Traumatic Brain Injury (TBI) is a prevalent form of neurological damage that may induce varying degrees of cognitive dysfunction in patients, consequently impacting their quality of life and social functioning. This article provides a mini review of the epidemiology in Chinese TBI patients and etiology of cognitive impairment. It analyzes the risk factors of cognitive impairment, discusses current management strategies for cognitive dysfunction in Chinese TBI patients, and summarizes the strengths and limitations of primary testing tools for TBI-related cognitive functions.

Probing amino acid side chains of the integral membrane protein PagP by solution NMR: Side chain immobilization facilitates association of secondary structures.

Solution NMR spectroscopy of large protein systems is hampered by rapid signal decay, so most multidimensional studies focus on long-lived H-C magnetization in methyl groups and/or backbone amide H-N magnetization in an otherwise perdeuterated environment. Herein we demonstrate that it is possible to biosynthetically incorporate additional H-C groups that possess long-lived magnetization using cost-effective partially deuterated or unlabeled amino acid precursors added to Escherichia coli growth media. This approach is applied to the outer membrane enzyme PagP in membrane-mimetic dodecylphosphocholine micelles.

Cost-effective selective deuteration of aromatic amino acid residues produces long-lived solution H NMR magnetization in proteins.

Solution NMR studies of large proteins are hampered by rapid signal decay due to short-range dipolar H-H and H-C interactions. These are attenuated by rapid rotation in methyl groups and by deuteration (H), so selective H,C-isotope labelling of methyl groups in otherwise perdeuterated proteins, combined with methyl transverse relaxation optimized spectroscopy (methyl-TROSY), is now standard for solution NMR of large protein systems > 25 kDa. For non-methyl positions, long-lived magnetization can be introduced as isolated H-C groups.

Ensemble calibration model of near-infrared spectroscopy based on functional data analysis.

As a nondestructive detection technology, near-infrared spectroscopy has been widely applied in various fields. With the wide application of near-infrared spectroscopy, the research on data processing has attracted more attention. Different from the existing discrete data model and based on the functional data analysis method, an ensemble calibration model FDA-EM-PLS (functional data analysis-ensemble learning-partial least squares) of near-infrared spectroscopy is proposed in this paper.

Fast online evaluation of vegetable freshness using single turnover chlorophyll fluorescence.

Vegetable freshness evaluation is of great significance to ensure the quality of vegetables and realize fine production. Existing vegetable freshness evaluation methods have difficulty realizing rapid online evaluation and industrial applications due to such disadvantages as being susceptible to subjective factors, complicated operation, large computation, and high hardware cost. To solve the above problems, a rapid online vegetable freshness evaluation method was developed based on the single turnover chlorophyll fluorescence parameters , / and .

Adjustable Handheld Probe Design for Photoacoustic Imaging:Experimental Validation.

Photoacoustic (PA) imaging is an emerging imaging technique that combines light illumination and ultrasound detection. Conventional design of PA probe usually combines light and sound in a straightforward way without adjustable capability. In this paper, we propose a new PA imaging system based on light-adjustable handheld probe.

Adjustable Handheld Probe Design for Photoacoustic Imaging: Mathematical Modelling and Simulation Study.

Photoacoustic imaging is an emerging imaging technique that combines light illumination and ultrasound detection. Conventional design of photoacoustic probe usually combines light and sound in a straightforward way without any adjustable capability. In this paper, we propose a new photoacoustic imaging system based on light-adjustable handheld probe.

Weighted partial least squares based on the error and variance of the recovery rate in calibration set.

The quantitative analysis is very difficult for the emission-excitation fluorescence spectroscopy of multi-component mixtures whose fluorescence peaks are serious overlapping. As an effective method for the quantitative analysis, partial least squares can extract the latent variables from both the independent variables and the dependent variables, so it can model for multiple correlations between variables. However, there are some factors that usually affect the prediction results of partial least squares, such as the noise, the distribution and amount of the samples in calibration set etc.

[Laser Induced Time-Resolved Fluorescence Spectroscopy Characteristics of Fluoranthene in Ethanol with Eanosecond Laser of 355 nm].

Polycyclic Aromatic Hydrocarbons (PAHs) have been widely investigated in environmental field, for most of them are mutagenic (carcinogenic, teratogenic, mutagenic). The influence of delay time and gate width on the time-resolved fluorescence spectroscopy of fluoranthene in ethanol was studied in this paper. Furthermore, laser induced time-resolved fluorescence spectroscopy of fluoranthene with different concentration were also researched.

Quantifying PAHs in water by three-way fluorescence spectra and second-order calibration methods.

It is still difficult to determine the concentrations of polycyclic aromatic hydrocarbons accurately in natural water by fluorescence technique because of their low solubility, different fluorescent intensity, and the complex interferents from water environments. In this work, three-way fluorescence spectra combined with three methods including three-way parallel factor analysis, multi-way partial least square with residual bilinearization and unfolded partial least square with residual bilinearization were used to predict the concentrations of polycyclic aromatic hydrocarbons at the μg L level in reservoir and river water, respectively. The prediction abilities of these methods on different analytes were evaluated by validation sets.

[Effect of Methanol-Water Mixture on Three-Dimensional Fluorescence Spectra of Carbaryl].

Extensive use of pesticides has a significant impact on the environment. Carbaryl, whose residues stay in the surface water, is one of the most widely used broad spectrum insecticides in agriculture. It is important to understand carbaryl spectral characteristics and detection methods.

Determination of Polycyclic Aromatic Hydrocarbons in the Presence of Humic Acid in water.

The presence of humic acid (HA) makes it extremely difficult to determine and quantify accurately polycyclic aromatic hydrocarbons (PAHs) in aquatic environment because of their complex and strong interaction. To solve this problem, a new method was developed in this work through the combination of PARAFAC and fluorescence spectroscopy, which mainly includes: (1) the fluorescence quantum yield acquisition of PAHs with and without HA by PARAFAC; (2) the  concentration score correction of PAHs in validation and test sets using the fluorescence quantum yields; and (3) the prediction of PAHs concentration in the validation and test sets in the presence of HA by corrected concentration. Using this method, the PAHs concentration on the level of µg L(-1) in the test samples with HA of 2.

[Outlier Detection of Time Series Three-Dimensional Fluorescence Spectroscopy].

The qualitative and quantitative analysis are often interfered by the outliers in time series three-dimensional fluorescence spectroscopy. In this work, an efficient outlier detection method is proposed by taking advantage of the characteristics in time dimension and the spectral dimension. Firstly, the wavelength points that are mostly the outliers are extracted by the variance in time dimension.

Determination of polycyclic aromatic hydrocarbons by four-way parallel factor analysis in presence of humic acid.

There is not effective method to solve the quenching effect of quencher in fluorescence spectra measurement and recognition of polycyclic aromatic hydrocarbons in aquatic environment. In this work, a four-way dataset combined with four-way parallel factor analysis is used to identify and quantify polycyclic aromatic hydrocarbons in the presence of humic acid, a fluorescent quencher and an ubiquitous substance in aquatic system, through modeling the quenching effect of humic acid by decomposing the four-way dataset into four loading matrices corresponding to relative concentration, excitation spectra, emission spectra and fluorescence quantum yield, respectively. It is found that Phenanthrene, pyrene, anthracene and fluorene can be recognized simultaneously with the similarities all above 0.

[Analysis of three-dimensional fluorescence overlapping spectra using differential spectra and independent component analysis].

The analysis of multi-component three-dimensional fluorescence overlapping spectra is always very difficult. In view of the advantage of differential spectra and based on the calculation principle of two-dimensional differential spectra, the three-dimensional fluorescence spectra with both excitation and emission spectra is fully utilized. Firstly, the excitation differential spectra and emission differential spectra are respectively computed after unfolding the three-dimensional fluorescence spectra.

Hydrothermal synthetic mercaptopropionic acid stabled CdTe quantum dots as fluorescent probes for detection of Ag⁺.

Mercaptopropionic acid (MPA) capped CdTe quantum dots (QDs) with particle size 3 nm have been successfully synthesized in aqueous medium by hydrothermal synthesis method. And the effects of different metal ions on MPA capped CdTe QDs fluorescence were studied using fluorescence spectrometry. The results demonstrated that at the same concentration level, Ag(+) could strongly quench CdTe QDs fluorescence, and the other metal ions had little effect on CdTe QDs fluorescence except Cu(2+).

A novel initialization method for nonnegative matrix factorization and its application in component recognition with three-dimensional fluorescence spectra.

Nonnegative matrix factorization has been widely used in many areas and has been applied for component recognition with three dimensional fluorescence spectra recently. However, nonnegative matrix factorization is a nonconvex programming in the iteration process, thus the solution is dependent on the initial values and consequently not unique. Up to now, an effective global convergent algorithm is still absent.

Integrated similarity indices for component recognition in mixtures with three-dimensional fluorescence spectra.

Component recognition is a very important issue in the analysis of mixed three-dimensional fluorescence spectra and it can be realized by calculating the similarity index between the reference spectra and the computed spectra from the trilinear decomposition of the three-dimensional data arrays. However, the most popular similarity index available in the literature for processing three-dimensional fluorescence spectra takes advantage of only part of the information from the trilinear decomposition. It works well when there are clear differences between the component spectra, but it may fail when the spectra are severely overlapped.

Application of excitation-emission matrix fluorescence combined with second-order calibration algorithm for the determination of five polycyclic aromatic hydrocarbons simultaneously in drinking.

Direct determination of five polycyclic aromatic hydrocarbons including fluorene, anthracene, phenanthrene, pyrene and fluoranthene was accomplished by excitation-emission matrix fluorescence coupled with chemometric methods based on an alternating trilinear decomposition (ATLD) algorithm. A uniform design and orthogonal design are proposed for the creation of the calibration set. Results showed that no significant difference in the recoveries for each of the PAHs was observed.