Classifications of anterior segment structure of congenital corneal opacity in infants and toddlers by ultrasound biomicroscopy and slit-lamp microscopic photographs: an observational study.

Background: The structural features have an impact on the surgical prognosis for congenital corneal opacity (CCO). The structural classification system of CCO, however, is lacking. Based on data from ultrasound biomicroscopy (UBM) findings in infants and toddlers with CCO, this research proposed a classification system for the anterior segment structure severity.

A New Treatment for Recalcitrant Neurotrophic Keratopathy of Ocular Graft-Versus-Host Disease with Virus Infection.

Introduction: Neurotrophic keratopathy (NK) is a rare degenerative ocular disease that can be difficult to treat. There were no effective resolutive treatments for severe NK caused by ocular graft-versus-host disease (oGVHD) along with virus infection. To address this question, we designed a prospective cohort study to evaluate the efficacy and safety of topical recombinant human nerve growth factor (rhNGF) in patients with recalcitrant NK of oGVHD and viral infection.

DALK combined intralamellar tectonic patch graft: an alternative approach to treat frank corneal perforation.

Background: Deep anterior lamellar keratoplasty (DALK) has gained popularity in cases of corneal thinning and leaking descemetocele. In this study, we introduced an intralamellar tectonic patch graft in addition to conventional DALK procedures to treat frank cornea perforation.

Methods: This retrospective case series included 13 patients (13 eyes) with frank corneal perforations who underwent DALK combined with intralamellar tectonic patch graft between December 2015 and December 2021.

Three-dimensional evaluation of the cornea in patients with unilateral posterior interstitial keratitis.

Purpose: The purpose of this study was to investigate the morphologic features of the cornea in patients with unilateral posterior interstitial keratitis.

Methods: Seven eyes of 7 patients with unilateral posterior interstitial keratitis were examined by slit-lamp biomicroscopy, anterior segment optical coherence tomography (AS-OCT), and confocal microscopy (IVCM). The imaging features of the cornea were evaluated and analyzed.

Assessing abnormal corneal endothelial cells from in vivo confocal microscopy images using a fully automated deep learning system.

Background: The goal of this study is to develop a fully automated segmentation and morphometric parameter estimation system for assessing abnormal corneal endothelial cells (CECs) from LASER in vivo confocal microscopy (IVCM) images.

Methods: First, we developed a fully automated deep learning system for assessing abnormal CECs using a previous development set composed of normal images and a newly constructed development set composed of abnormal images. Second, two testing sets, one with 169 normal images and the other with 211 abnormal images, were used to evaluate the clinical validity and effectiveness of the proposed system on LASER IVCM images with different corneal endothelial conditions, particularly on abnormal images.

A Fully Automated Segmentation and Morphometric Parameter Estimation System for Assessing Corneal Endothelial Cell Images.

Purpose: To develop a fully automated segmentation and morphometric parameter estimation system for assessing corneal endothelial cells from in vivo confocal microscopy images.

Design: Artificial intelligence (neural network) study.

Methods: First, a fully automated deep learning system for assessing corneal endothelial cells was developed using the development set (from 99 subjects).

Severe Corneal Edema Increases ECL From Grafts After DSAEK-Corneal Edema and ECL After DSAEK.

Objectives: To determine the relationship between the preoperative degree of corneal edema in the recipient and the endothelial cell density in grafts after Descemet stripping automated endothelial keratoplasty (DSAEK).

Methods: This retrospective case series enrolled 111 eyes of 107 patients who underwent DSAEK. The preoperative and postoperative central corneal thickness (CCT) was measured by anterior-segment optical coherence tomography.

Fully automated grading system for the evaluation of punctate epithelial erosions using deep neural networks.

Purpose: The goal was to develop a fully automated grading system for the evaluation of punctate epithelial erosions (PEEs) using deep neural networks.

Methods: A fully automated system was developed to detect corneal position and grade staining severity given a corneal fluorescein staining image. The fully automated pipeline consists of the following three steps: a corneal segmentation model extracts corneal area; five image patches are cropped from the staining image based on the five subregions of extracted cornea; a staining grading model predicts a score for each image patch from 0 to 3, and automated grading score for the whole cornea is obtained from 0 to 15.

A short-term study of corneal collagen cross-linking with hypo-osmolar riboflavin solution in keratoconic corneas.

Aim: To report the 3mo outcomes of collagen cross-linking (CXL) with a hypo-osmolar riboflavin in thin corneas with the thinnest thickness less than 400 µm without epithelium.

Methods: Eight eyes in 6 patients with age 26.2±4.

Corneal collagen cross-linking with hypoosmolar riboflavin solution in keratoconic corneas.

Purpose: To report the 12-month outcomes of corneal collagen cross-linking (CXL) with a hypoosmolar riboflavin and ultraviolet-A (UVA) irradiation in thin corneas.

Methods: Eight eyes underwent CXL using a hypoosmolar riboflavin solution after epithelial removal. The corrected distance visual acuity (CDVA), manifest refraction, the mean thinnest corneal thickness (MTCT), and the endothelial cell density (ECD) were evaluated before and 6 and 12 months after CXL.

[Immunocompetence effects of polysaccharide of snakegourd root on human peripheral blood mononuclear cells in vitro].

Objective: To establish the method of promoting human peripheral blood mononuclear cell proliferation by polysaccharide of snakegourd root and identify the effects of polysaccharide of snakegourd root on lymphocyte proliferation, T lymphocyte subsets and the different levels of TNF-alpha and IL-6.

Method: The polysaccharide of snakegourd root preparations were purified with dialysis and ethanol precipitation. The healthy human PBMC were used as the target cells for screening potency of the drugs.

Differentiation of rabbit bone marrow mesenchymal stem cells into corneal epithelial cells in vivo and ex vivo.

Purpose: To examine whether bone marrow mesenchymal stem cells (MSCs) could be differentiated into corneal epithelial cells in vivo and ex vivo.

Methods: In vivo, BrdU labeled rabbit MSCs (Rb-MSCs) were suspended in the fibrin gels and transplanted onto the surface of the damaged rabbit corneas. Histology and molecular phenotype were studied on postoperative day 28.

[Experimental research of small-incision Descemet's stripping endothelial keratoplasty].

Objective: To investigate the surgical procedure, clinical efficacy, complications, density of endothelial cells and histological changes after Descemet's stripping endothelial keratoplasty (DSEK) surgery.

Methods: It was a experimental study. Twenty four New Zealand rabbits were divided into 3 groups, 8 rabbits per group.

[The proapoptotic effect of the homogenate of the tissue of different parts of pig's full thickness dermal wounds on cultured fibroblasts].

Objective: To observe the proapoptotic effect of the homogenate of different parts of pig's full thickness dermal wounds on cultured fibroblasts.

Methods: The tissues were dissected from the wound center and sub-neoepithelium separately 15 days after homogenization and sterilization, the specimens stored at -70 degrees C. The forth passage of the fibroblasts were cultured for 16 hours in different culture solutions and were grouped into 7 groups: DMEM containing 5% fetal bovine serum as Group I, DMEM containing 5% homogenate of tissue from wound center as Group II, DMEM containing 5% homogenate of tissue from sub-neoepithelium as Group III, the culture solution of Group I mixed with 10 microg/ml GM6001 in Group IV, with the culturing medium of Group III plus 10 microg/ml GM6001 as Group V, the culture solution of Group II mixed with 10 ng/ml aFGF as Group VII, and the culture solution of Group III mixed with 10 ng/ml aFGF as Group VII.

[Culture and identification of human skin fibroblasts].

Objective: To explore the method of isolation, cultivation, and identification of human skin fibroblasts in vitro.

Methods: By digesting human skin with collagenase type II to isolate human eccrine sweat glands. The fibroblasts grew along with the growth of eccrine sweat gland cells,and they were separated by digesting with 0.

[Isolation and purification of eccrine sweat glands in human skin].

Objective: To investigate the method of isolation and purification of epithelial cells of human eccrine sweat gland in vitro.

Methods: Through digesting human skin with collagenase type II, cells of human eccrine sweat gland were isolated. Highly purified gland cells were obtained through transferring into the conditioned medium with a micropipette for at least three times under an inverted microscope.