Impact of the interaction between herpes simplex virus 1 ICP22 and FACT on viral gene expression and pathogenesis.

Facilitates chromatin transcription (FACT) interacts with nucleosomes to promote gene transcription by regulating the dissociation and reassembly of nucleosomes downstream and upstream of RNA polymerase II (Pol II). A previous study reported that herpes simplex virus 1 (HSV-1) regulatory protein ICP22 interacted with FACT and was required for its recruitment to the viral DNA genome in HSV-1-infected cells. However, the biological importance of interactions between ICP22 and FACT in relation to HSV-1 infection is unclear.

Spectral-Spatial Feature Fusion for Hyperspectral Anomaly Detection.

Hyperspectral anomaly detection is used to recognize unusual patterns or anomalies in hyperspectral data. Currently, many spectral-spatial detection methods have been proposed with a cascaded manner; however, they often neglect the complementary characteristics between the spectral and spatial dimensions, which easily leads to yield high false alarm rate. To alleviate this issue, a spectral-spatial information fusion (SSIF) method is designed for hyperspectral anomaly detection.

Learning to Guide Particle Search for Dynamic Multiobjective Optimization.

Dynamic multiobjective optimization problems (DMOPs) are characterized by multiple objectives that change over time in varying environments. More specifically, environmental changes can be described as various dynamics. However, it is difficult for existing dynamic multiobjective algorithms (DMOAs) to handle DMOPs due to their inability to learn in different environments to guide the search.

Total Syntheses of Uncommon C Terpenoids: Chamaecydin and Isochamaecydin.

(-)-Chamaecydin and (-)-isochamaecydin, two uncommon C terpenoids comprising abietane-type diterpenes and thujane-type monoterpenes, were achieved from β-pinene with (-)-sabinene in 18 and 20 steps, respectively. Key steps include a Claisen-Ireland rearrangement to establish the all-carbon quaternary center, a Rh catalyzed C-H bond insertion reaction to install a spiro-five-membered ring and a Lewis acid promoted cyclization of polyenes to construct the two six-membered rings.

Ratiometric photon reassignment based on fluorescence lifetime to improve resolution in pulse STED microscopy.

Depletion beams with long pulse durations (∼600) have recently been applied in stimulated emission depletion (STED) microscopy to reduce photobleaching and phototoxicity. Therefore, improving the resolution of pulse STED at lower depletion power for the super-resolution imaging of live biological specimens has attracted increasing interest. Herein, we present a simple method termed ratiometric photon reassignment based on the fluorescence lifetime, in which highly spatially resolved long-lifetime fluorophore components in the center are extracted, and short-lifetime fluorophore components in the periphery are discarded to improve resolution without increasing the depletion power.

Ultrasound-assisted electrochemical treatment for phenolic wastewater.

With the rapid development of industry, especially the rapid rise of the chemical industry, the problem of water pollution is becoming more and more serious. Among them, the discharge of organic pollutants represented by phenolic substances has always been at the forefront. In this paper, ultrasound-assisted electrochemical treatment for phenolic wastewater is investigated.

Label-free difference super-resolution microscopy based on parallel detection.

In this paper, a new method is proposed for super-resolution imaging of non-fluorescent samples. This approach is based on the intensity difference between confocal image and negative confocal image, which are simultaneously acquired at one sample scanning. In order to get these two different images simultaneously, the sample was illuminated by two different focused spots from the same laser source: the doughnut spot and the solid spot.

Fluorescence emission difference microscopy by superoscillation excitation.

A superresolution fluorescence emission difference (FED) microscopy based on superoscillation excitation is investigated. The solid superoscillation excitation spot is produced by the radially polarised Laguerre-Gaussian beam (LG), and the donut superoscillation excitation spot is produced by the same LG beam with spiral phase modulation. We show that the superoscillation excitation can enhance the spatial resolution of FED microscopy.

Improving the resolution of two-photon microscopy using pixel reassignment.

An imaging system combining parallel detection and two-photon excitation is presented in this paper. In the proposed method, seven avalanche photodiodes (APD) are used in the detection path. The light is guided onto the APD sensor by a fiber bundle with seven fibers.

Two-photon microscopy with enhanced contrast and resolution.

A method combining the saturation effect with the ratio concerned quadratic intensity weighted subtraction (RQIWS) algorithm for resolution and contrast enhancement in a two-photon microscopy system is presented in this paper. In the proposed method, the saturation effect is utilized to get a profile-extended solid spot and a center-shrunken doughnut-shaped spot for a smaller effective point spread function, which enhances the resolution of the system. The RQIWS algorithm uses the intensity ratio of the two original images acquired respectively with the solid spot and the doughnut-shaped spot as one of the subtraction parameters and takes the fluorescence quadratic dependence of excitation intensity into account for better subtraction results compared with the intensity weighted subtraction algorithm in a two-photon excitation system.

[Research on granary temperature network monitoring system based on the linear frequency shift of spectrum].

In order to obtain the distribution of the temperature in the range of the granary precisely and stably, we designed a temperature measurement system of the fiber Bragg grating. Through the fiber-optic network the system can detect granary temperature in a wide range, and there is a linear relationship between the measured temperature by fiber Bragg grating and the center wavelength, so according to the function of spectrum linear frequency shift it obtained precise temperature in the granary. The working wavelengths of each grating in the system are separated from each other.

Determination of perfluorinated compounds in packaging materials and textiles using pressurized liquid extraction with gas chromatography-mass spectrometry.

A simultaneous determination method of trace amounts of perfluorinated compounds, such as perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS) in packaging materials and textiles, has been developed, using pressurized liquid extraction (PLE) with gas chromatography-mass spectrometry (GC/MS). The perfluorinated compounds were primarily extracted from the samples by a PLE procedure, in which the parameters were optimized by response surface methodology. The solvent was then removed by blowing nitrogen and a silylation step was carried out with N,N-bis(trimethylsilyl)trifluoroacetamide.