Publications by authors named "ShaoJian Xu"

Article Synopsis
  • - Underground mining is risky, with dangers like collapses and gas leaks, so a new indoor localization system using Bluetooth and ultrasonic technology has been developed to ensure worker safety, especially when satellite signals are unavailable.
  • - The system combines ultrasonic receiver arrays with an advanced algorithm for accurate positioning and incorporates GIS (Geographic Information Systems) analysis to optimize the mining layout while tracking movement with an inertial navigation module.
  • - In experiments, the proposed method achieved a positioning accuracy of within 10 cm, which is 20% better than traditional techniques, making it highly beneficial for safe automated operations in underground mines.
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Bluetooth devices have been widely used for pedestrian positioning and navigation in complex indoor scenes. Bluetooth beacons are scattered throughout the entire indoor walkable area containing stairwells, and pedestrian positioning can be obtained by the received Bluetooth packets. However, the positioning performance is sharply deteriorated by the multipath effects originating from indoor clutter and walls.

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The SARS-CoV-2 Omicron variant sparked the largest wave of infections worldwide. Mainland China eased its strict COVID-19 measures in late 2022 and experienced two nationwide Omicron waves in 2023. Here, we investigated lineage distribution and virus evolution in Guangdong, China, 2022-2023 by comparing 5813 local viral genomes with the datasets from other regions of China and worldwide.

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The HIV-1 envelope glycoprotein (Env) plays crucial role in viral infection by facilitating viral attachment to host cells and inducing fusion of the virus with the host cell membrane. This fusion allows the HIV-1 viral genome to enter the target cell then triggering various stages of the viral life cycle. The native Env directly interacts with the main receptor CD4 and the co-receptor (CCR5 or CXCR4) in human cell membrane then induces membrane fusion.

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  • * A study of over 36,000 EV-positive samples in Guangdong from 2013 to 2021 reveals that Coxsackievirus A6 and A10 have become the leading causes of HFMD, while the incidence of EV-A71 has drastically decreased since 2018.
  • * Genetic analysis identified a specific mutation in Coxsackievirus A10 and highlighted the necessity for enhanced surveillance and changes in vaccination strategies to adapt to the evolving landscape of enteroviruses
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Porcine circovirus type 2 (PCV2) is a globally prevalent infectious pathogen affecting swine, with its capsid protein (Cap) being the sole structural protein critical for vaccine development. Prior research has demonstrated that PCV2 Cap proteins produced in Escherichia coli (E. coli) can form virus-like particles (VLPs) in vitro, and nuclear localization signal peptides (NLS) play a pivotal role in stabilizing PCV2 VLPs.

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Near-infrared (NIR) phosphors with high thermal stability are significant for NIR light-emitting diodes (LEDs). For a decade, Debye temperature has been a successful indicator of structural rigidity and thermal stability for phosphors, but some exceptions exist due to its dependence on atomic mass. Inspired by the Debye temperature model that relates the elastic properties of solids, our density functional theory calculations revealed that the Vickers hardness of Cr-doped NIR phosphors was negatively correlated with Stokes shifts (Pearson's = -0.

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Article Synopsis
  • The study explores how the transition from disordered to ordered structural states in phosphors like CaGaGeO:Cr and its variants enhances both luminescence efficiency and thermal stability.
  • A paradox is identified, as there is an unexpected relationship between the disorder levels and the improvements in these properties, suggesting that it's the framework disorder specifically, rather than overall lattice disorder, that plays a crucial role.
  • By analyzing structural, optical, and theoretical aspects, the research highlights the importance of framework-order engineering as a strategy to develop high-performance near-infrared phosphors.
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The epigenetic modification of the N6-methyladenosine (mA) methylation plays an important role in virus infection and replication. However, its role in Porcine circovirus type 2 (PCV2) replication has not been well studied. Here, we demonstrated that mA modifications are increased in PK-15 cells after PCV2 infection.

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Senecavirus A (SVA), formerly called Seneca Valley virus (SVV) was first isolated from the USA in 2002. This study isolated an SVA strain from a pig herd in Shandong Province, PR China and designated it SVA-CH-SDGT-2017. The full-length genome, excluding the poly(A) tails of the SVA isolates, was 7280 nucleotides long, with the genomic organization resembling and sharing high nucleotide identities of 90.

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A chimeric PCV called PCV1-3 with the immunogenic Cap gene of pathogenic PCV type 3(PCV3) cloned into the genomic skeleton of the nonpathogenic PCV1 was rescued and inoculated into PCV3 negative piglets. The results of fluorescence quantitative PCR showed that the PCV1-3 DNA detected in serum and tissues was negative. The pathogenicity of piglets showed that PCV 1-3 did not cause the clinical characteristics and pathological changes.

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RNA interference (RNAi) is a major form of antiviral defense in host cells, and Ago2 and Dicer are the major proteins of RNAi. The Senecavirus A (SVA) is a reemerging virus, resulting in vesicular lesions in sows and a sharp decline in neonatal piglet production. In this study, CRISPR/Cas9 technology was used to knock out Ago2 and Dicer genes in BHK-21 cell lines used for SVA vaccine production.

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Porcine circovirus type 2 (PCV2) is the etiological agent of post-weaning multisystemic wasting syndrome (PMWS). The original prevalent genotype, PCV2a, has been replaced by genotypes 2b and 2d in the swine population worldwide. The Rep protein is critical for viral replication.

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The endoplasmic reticulum (ER) plays an essential role in Ca concentration balance and protein biosynthesis. During infection, the virus needs to complete its life process with the help of ER. At the same time, ER also produces ER stress (ERS), which induces apoptosis to resist virus infection.

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Porcine circovirus type 2 (PCV2), a ubiquitous pathogen that primary cause of postweaning multisystemic wasting syndrome (PMWS), had caused significant morbidity and mortality in swine populations with huge economic losses in the worldwide swine industry. Currently, looking for effective antiviral drugs for PCV2 infection remains an important works. In our study, CRISPR/Cas9 system was used to further detected the key sites of PCV2 replication.

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In this study, we investigated the epidemiology and molecular characteristics of enteroviruses associated with severe hand, foot and mouth disease (HFMD) in Shenzhen, China, during 2014-2018. A total of 137 fecal specimens from patients with severe HFMD were collected. Enterovirus (EV) types were determined using real-time reverse transcription polymerase chain reaction (RT-PCR), RT nested PCR, and sequencing.

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To expand the toolbox for the synthesis of -phenolic sulfilimines, sigmatropic rearrangements were introduced to the field of sulfilimine chemistry. Herein we report a N-H sulfenylation/[2,3]-sigmatropic rearrangement cascade reaction. This mild reaction enables commercially available thiols to serve as the sulfenylation reagent and generates water as the sole byproduct.

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Article Synopsis
  • * Researchers analyzed 40 genome sequences of PCV2 from Shandong Province, revealing the coexistence of three genotypes (2a, 2b, 2d), with PCV2d emerging as the dominant type.
  • * Analysis of amino acid sequences indicated distinct sites that differentiate the genotypes, suggesting that the rising prevalence of PCV2d might lead to it outcompeting PCV2b as the main strain in the region.
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The Type VI secretion system (T6SS) is a membrane protein complex related to inter-bacterial competitions and host-pathogen interactions in Pseudomonas aeruginosa. The T6SS is regulated by a great variety of regulatory mechanisms at multiple levels, including post-translational modification with threonine phosphorylation mediated by Ser/Thr protein kinase PpkA and phosphatase PppA. The T6SS is activated by PpkA via Thr phosphorylation of Fha, and PppA can antagonize PpkA.

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Coxsackievirus group A (CV-A) strains are important pathogens of hand, foot, and mouth disease and herpangina. We report here the near-complete genome sequences of 12 CV-A strains isolated from infants and children with different clinical diseases. The presented data will be very useful for future genome-based epidemiological studies.

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Article Synopsis
  • Coxsackievirus A16 (CV-A16) genotypes B1a and B1b have been prevalent in mainland China, with data collected from 3,008 patients suffering from hand, foot, and mouth disease between 2013 and 2017.
  • The overall detection rate of CV-A16 was 16.5%, showing fluctuations over the years, while a significant majority of the detected strains belonged to subgenotype B1b.
  • Two strains were identified as novel and placed in a new clade (subgenogroup B3), with phylogenetic analysis revealing additional new genotypes B1d and B4 among global CV-A16 strains.
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Porcine circovirus (PCV) has two potential open reading frames, ORF1 and ORF2. ORF1 is predicted to encode a replication-associated protein (Rep) essential for replication of viral DNA. In some studies, PCV1 replicated more efficiently in PK-15 cells than PCV2 was elucidated.

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Here, we report the complete genome sequences of four coxsackievirus A16 strains isolated from four children with severe hand, foot, and mouth disease. Three of them were assigned to subgenotype B1b based on phylogenetic analysis of the VP1 gene, and the other one belonged to subgenotype B1a.

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Background: This study aimed at reseaching the immune effect of porcine circovirus type 2 (PCV2) DNA vaccine containing CpG motif on mice.

Methods: A total of 40 6-week-old female BALB/c mice were randomly divided into four groups which were immunized by 18CpG-pVAX1-ORF2, pVAX1-ORF2, pVAX1 and PBS, respectively, and immunized again 2 weeks later. All mice were challenged with 0.

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Nowadays, adjuvant is still important for boosting immunity and improving resistance in animals. In order to boost the immunity of porcine circovirus type 2 (PCV2) DNA vaccine, CpG motifs were inserted. In this study, the dose-effect was studied, and the immunity of PCV2 DNA vaccines by recombinant open reading frame 2 (ORF2) gene and CpG motifs was evaluated.

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