[Effects of RhoA siRNA on the proliferation, adhesion, migration and invasion of tongue squamous cell carcinoma Tca8113 cells in vitro].

Objective: to investigate the effect of RhoA on the metastasis of tongue squamous cell carcinoma Tca8113 cells in vitro.

Methods: a group of RhoA specific small interfering RNAs (siRNA) was constructed and confined by sequencing analysis. The siRNA of RhoA gene was transfected into human tongue squamous cell carcinoma Tca8113 cells line by Lipofectamine(TM) 2000.

[Coexpression of Schwann cell marker GFAP and myoepithelial cell marker alpha-SMA in salivary adenoid cystic carcinoma].

Objective: To observe the expression of Schwann cell marker GFAP and myoepithelial cell marker alpha-SMA in salivary adenoid cystic carcinoma (ACC), and to evaluate the relationship of GFAP, alpha-SMA and perineural invasion in ACC.

Methods: Immunohistochemical SABC method, double-label immunofluorescence and CLSM were used to detect the expression of GFAP and alpha-SMA proteins in salivary ACC tissue samples.

Results: In salivary ACC tissue samples, both GFAP and alpha-SMA proteins were positive, which were coexpressed in cytoplasm of the same onco-myoepithelial cells.

[Anti-tumor immune response in vitro induced by fusion of Tca8113 cells with macrophages].

Objective: To evaluate the therapeutic effectiveness of fusion tumor vaccine in tongue cancer treatment.

Methods: Human macrophages fused with human tongue carcinoma cell line Tca8113 cell. The fusion cells were selected by magnetic cell sorting (MACS) and cultured.

[Construction and expression of eukaryotic expression vector containing hVEGF165 gene in rat bone marrow stroma cell].

Aim: To construct the eukaryotic expression vector containing human vascular endothelial growth factor 165(VEGF165) gene and express it in rat bone marrow stroma cells(rMSCs).

Methods: The recombinant plasmid pSP73-VEGF165 was digested with BamH I and Xho I. Then the hVEGF165 gene segment obtained was again cloned into pcDNA3.

[Structural mechanism studies of hTNF alpha mutants in position 30 and 42 amino acid].

Objective: To study the relationship between the structure and functional activity of hTNF alpha.

Methods: Four hTNF alpha mutants were constructed, different binding structures and gene responses related with these mutants were studied by the methods of immunoprecipitation and mRNA differential display.

Results: The specific activities and LD50 of the different hTNF alpha mutants indicated their different bioactivities.