[Cloning and Expression Analysis of Acetyl-CoA C-acetyltransferase Gene in Isodon rubescens].

Objective: To clone the acetyl-CoA C-acetyl transferase( AACT) gene from Isodon rubescens, and to analyze the bioinformatics and expression of the gene.

Methods: According to the IrAACT gene sequence of Isodon rubescens transcriptome,a pair of primers was designed, and the ORF of cDNA sequence was obtained by reverse transcription PCR. Bioinformatic analysis of this gene and its corresponding protein were performed.

[Estimating Winter Wheat Nitrogen Vertical Distribution Based on Bidirectional Canopy Reflected Spectrum].

The vertical distribution of crop nitrogen is increased with plant height, timely and non-damaging measurement of crop nitrogen vertical distribution is critical for the crop production and quality, improving fertilizer utilization and reducing environmental impact. The objective of this study was to discuss the method of estimating winter wheat nitrogen vertical distribution by exploring bidirectional reflectance distribution function (BRDF) data using partial least square (PLS) algorithm. The canopy reflectance at nadir, +/-50 degrees and +/- 60 degrees; at nadir, +/- 30 degrees and +/- 40 degrees; and at nadir, +/- 20 degrees and +/- 30 degrees were selected to estimate foliage nitrogen density (FND) at upper layer, middle layer and bottom layer, respectively.

[Causes of moderate to severe visual impairment and blindness in population aged 50 years or more in rural Shandong province].

Objective: To investigate the etiological spectrum of moderate to severe visual impairment and blindness in population aged 50 years or more in rural Shandong province, China.

Methods: A population based, random cluster sampling was used to screening the adults aged 50 years or more living in rural Shandong Province from April to July 2008. Three counties and one suburb representing the different levels of socioeconomic development within Shandong area were selected as the investigated areas.

[Prevalence of visual impairment and blindness in older adults in rural Shandong Province].

Objective: To describe the prevalence of visual impairment/blindness among older adults aged > or = 50 years in rural populations in Shandong Province, China.

Methods: Population-based, cross-sectional study was designed in this survey. Three counties and one suburbs representative of the different levels of socioeconomic development were selected as the study areas within the province from April to July 2008.