Bioinformatic analysis identifies key transcriptome signatures in temporal lobe epilepsy.

Aims: To identify transcriptome signatures underlying epileptogenesis in temporal lobe epilepsy (TLE).

Methods: Robust rank aggregation analysis was used to integrate multiple microarrays in rodent models of TLE and determine differentially expressed genes (DEGs) in acute, latent, and chronic stages. Functional annotation and protein-protein interaction analysis were performed to explore the potential functions of the DEGs and identify hub genes with the highest intramodular connectivity.

[In vivo and in vitro research study of the influence on placenta angiogenesis by gene silencing of netrin-1].

Objective: To study influence on angiogenesis of placenta by gene silencing of netrin-1.

Methods: Netrin-1 gene in human umbilical vein endothelial cells (HUVEC) and placenta of pregnant rats were silenced by RNA interference. The following methods were used in this study, including the phenytetrazoliumromide (MTT) for viability, clone formation for proliferation, transwell for migration, and tube formation for angiogenesis in vitro.

Reduced expression of netrin-1 is associated with fetal growth restriction.

The objective of this study was to investigate the expression of netrin-1 in placenta from patients with fetal growth restriction (FGR) and its effect on the viability and apoptosis of human placental microvascular endothelial cells. Thirty-three patients with FGR (including eighteen severe cases) and twenty-four normal late pregnant women were investigated. The expression of netrin-1 in placental tissues was detected by employing immunohistochemistry, real-time PCR, and Western blotting.