[The relationship between RUNX3 gene mutation and keloid].

Objective: To study the mutation in RH120480 fragment of RUNX3 gene among the Chinese patients with keloid.

Methods: 20 samples of keloids were collected with each patient's venous blood sample as normal control group. The genomic DNA was extracted from each sample.

[Comparative genomic hybridization analysis of nonsyndromic cleft lip with palate].

Objective: To identify the genetic alterations in nonsyndromic cleft lip and palate (NSCLP).

Methods: Comparative genomic hybridization was applied to investigate the genomic imbalance (the gain or loss of genetic material) in 7 cases of NSCLP.

Results: It showed that the loss of chromosome DNA copies happened in chromosome 6, 7, 10, 13, 14, 16, 20, 22 and the gain of chromosome DNA copies happened in chromosome 5, 15, 18, 19.

[Effects of 5F from Pteris semipinnate L on growth of human pathological scars in nude mice].

Objective: To investigate the effect of 5F from Pteris semipinnate L on the growth of human pathological scar in nude mice.

Methods: 5F from Pteris semipinnate L was administered at different doses in nude mouse models bearing human pathological scars. The morphology, histology, tumor growth factor-beta1 and type I collagen content of the scar tissues were examined after the administration.

[The function of basic fibroblastic growth factor on revascularization of pearl fat graft transplantation].

Objective: To investigate the function of basic fibroblastic growth factor on the survival of fat transplantation.

Methods: Basic fibroblastic growth factor was used in pearl fat graft transplantation on experimental animal models. Microvessels densities both on experimental sides and control sides were quantitatively researched in various periods.

[Chromosomal aberration in human keloid analyzed by comparative genomic hybridization].

Objective: To identify the genetic alteration in human keloid.

Methods: Comparative genomic hybridization was applied in 6 cases of keloid to investigate the genomic imbalance (the gain or loss of genetic material).

Results: The study showed that the loss of chromosome DNA copies included chromosome, 1,7,9,13,16,17,18,19,20,22.

Effects of antisense oligonucleotides of PKC-alpha on proliferation and apoptosis of HepG2 in vitro.

Background: Hepatocellular carcinoma (HCC) is one of the most common malignancies in China. The long-term survival rate of patients with HCC after prevention and management remains unsatisfactory. In order to provide a novel strategy to cure HCC, we investigated the effects of antisense oligonucleotides of PKC-alpha on proliferation and apoptosis of human hepatoma cell line HepG2 in vitro.

[The TGF-alpha gene Taq I polymorphism and non-syndromic cleft lip with or without cleft palate].

Objective: To study the association of TGF-alpha gene Taq I polymorphism and nonsyndromic cleft lip with or without cleft palate (NSCL/P) in Chinese.

Methods: 107 patients with NSCL/P and 136 healthy controls were examined for TGF-alpha/Taq I genotypes. TGF-alpha/Taq I typing was carried out by digesting the locus specific polymerase chain reaction amplified products with alleles specific Taq I restriction enzyme (PCR-RELP).

[The expression of EST and ER in hemangioma with its clinical value].

Objective: To study the relationship between estrogen and the development of hemangioma.

Methods: The expression of EST and ER in samples from the thirty-eight cases of hemangioma and six cases of normal control group was examined with the immunohistochemical steptavidin peroxidase conjugated method (SP method).

Results: The EST in capillary hemangioma expressed significantly higher than in the cavernous hemangioma, the racemose hemangioma or the control group.