The Ras/ERK signaling pathway couples antimicrobial peptides to mediate resistance to dengue virus in Aedes mosquitoes.

Aedes mosquitoes can transmit dengue and several other severe vector-borne viral diseases, thereby influencing millions of people worldwide. Insects primarily control and clear the viral infections via their innate immune systems. Mitogen-Activated Protein Kinases (MAPKs) and antimicrobial peptides (AMPs) are both evolutionarily conserved components of the innate immune systems.

[Preparation and antigenicity analysis of recombinant aegyptin-like protein of Aedes albopictus].

Objective: To clone and express the aegyptin-like protein (alALP) encoding gene from Aedes albopictus salivary gland, and analyze its antigenicity.

Methods: The homology, secondary structure and antigen peptides of alALP and aegyptin protein (GenBank No. ABF18122.

[Preparation and application of the polyclonal antibody of Toxoplasma gondii autophagy protein 8 (TgAtg8)].

Objective: To prepare and evaluate specific-TgAtg8 polyclonal antibody.

Methods: The known Saccharomyces cerevisiae Atg protein sequences were used to identify Toxoplasma gondii homologous protein through bioinformatics analysis. TgAtg8 cDNA was amplified and cloned into prokaryotic expression vector pGEX-6p-1.

Specific detection of Angiostrongylus cantonensis in the snail Achatina fulica using a loop-mediated isothermal amplification (LAMP) assay.

Angiostrongylus cantonensis, a rat lungworm, can cause eosinophilic meningitis and angiostrongyliasis in humans following ingestion of contaminated foods or intermediate/paratenic hosts with infective larvae. The snail Achatina fulica is one of the important intermediate hosts of A. cantonensis and is commonly eaten by humans in some countries.

[Microbial survey of bacterial contamination of shellfish products in Wenzhou].

Objective: To investigate the status of bacterial contamination in the shellfish products in Wenzhou.

Methods: One hundred samples were collected and their bacterial populations including the total plate count were investigated.

Results: Of the 100 samples collected, 67 samples failed to not meet the national regulations due to bacterial contamination, accounting for 67% of the total samples.

[Expression and antigenicity analysis of NTPase gene of Toxoplasma gondii].

Objective: To clone and express prokaryotic recombinant plasmid of nucleoside triphosphate hydrolase (NTPase) gene of Toxoplasma gondii, and analyze its antigenicity.

Method: NTPase gene was amplified by PCR from RH strain of T. gondii and cloned into pGEM-T Easy vector.

[Preparation, characterization and preliminary application of recombinant protein AP33 of trichomonas vaginalis].

Objective: To clone ap33 gene of Trichomonas vaginalis( T. v), construct prokaryotic expression system of the gene and identify its antigenicity and immunogenicity.

Methods: The total RNA was extracted from a clinical isolate Tv317 and the cDNA was synthesized by reverse transcription.

[Detection of Angiostrongylus cantonensis circulating antigen by monoclonal antibodies].

Objective: To prepare monoclonal antibodies (McAbs) against soluble antigens of adult worms of Angiostrongylus cantonensis (A. cantonensis) on the purpose to detect CAg of A. cantonensis.

Construction of expression systems for flaA and flaB genes of Helicobacter pylori and determination of immunoreactivity and antigenicity of recombinant proteins.

Aim: To clone flagellin genes A (flaA) and B (flaB) from a clinical strain of Helicobacter pylori (H pylori) and to construct prokaryotic expression systems of the genes and identify immunity of the fusion proteins.

Methods: The flaA and flaB genes from a clinical H pylori isolate Y06 were amplified by high fidelity PCR. The nucleotide sequences of target DNA amplification fragments from the two genes were sequenced after T-A cloning.

[Cloning, expression and identification of flaB gene from a clinical isolate of Helicobacter pylori].

Objective: To clone Helicobacter pylori flagellin B gene (flaB) to construct prokaryotic expression system of the gene and to identify immunity of the fusion protein.

Methods: The flaB gene from a clinical isolate Y06 of H.pylori was amplified by high fidelity PCR.