[Study on the production of trehalose by bacterium D-97 endocellular enzymes using HPLC/RI and HPLC/ESI-MS].

The mechanism in which trehalose is produced from dextrin or starch hydrolyzate by endocellular enzymes of bacterium D-97 can be elucidated high performance liquid chromatography (HPLC) with differential refraction detection (RI) basically, including the effect of the different carbon sources on the endocellular trehalose-producing enzymes in bacterium D-97 and the possibility or ability of the endocellular enzymes to produce trehalose using maltooligosaccharides of different chain lengths. After purification of endocellular enzymes of bacterium D-97, two enzymes (called Enzyme A and Enzyme B) related to trehalose synthesis were found. The unknown oligosaccharides produced by Enzyme A were analyzed with the HPLC/RI and HPLC/ESI-MS (electrospray ionization mass spectrometry).