Evaluation of the performance of the EIAgen HCV test for detection of hepatitis C virus infection.

The EIAgen HCV test (Adaltis Inc., Montreal, Canada) is an enzyme immunoassay (EIA) for the detection of anti-hepatitis C virus (HCV) antibodies. This study compared the performance of this test side-by-side with the current Ortho HCV 3.

[Hepatitis B surface antigen terminates codon bias selection].

Objective: To investigate the existence condition of hepatitis B surface antigen(HBsAg) termination codon bias.

Methods: A total of 174 reference sequences of all kinds of Hepatitis B virus(HBV) genotypes were chosen from GenBank, and compared by BioEdit. Then secondary structure of RNA was constructed and analyzed together.

Senescence marker protein 30 in acute liver failure: validation of a mass spectrometry proteomics assay.

Background: Our previous proteomic study showed that the senescence marker protein (SMP30) is selectively present in the plasma of a murine model of acute liver failure (ALF). The aim of this study was to validate this SMP30 expression in the plasma and liver tissues of mice and humans with ALF.

Methods: After the proteomic analysis of plasma from a murine model of D-galactosamine/lipopolysaccharide (GalN/LPS)-induced ALF by two-dimensional electrophoresis (2-DE) and mass spectrometry, the expression levels of SMP30 in the plasma and liver tissues were validated by western blot and RT-PCR analyses.

[Concordance between hepatitis C virus serotype and genotype in chronic hepatitis C patients in China].

Objective: To investigate the relationship of hepatitis C virus (HCV) serotype with genotype.

Methods: The serotypes of HCV in the serum of 104 patients with chronic hepatitis C from 14 cities in China for which HCV genotypes were available, were determined by ELISA using Murex HCV Serotyping 1-6 Assay.

Results: The serotypes of 86 (82.

The functional evaluation of dendritic cell vaccines based on different hepatitis C virus nonstructural genes.

Hepatitis C virus (HCV) nonstructural (NS) genes are relatively conserved and play critical roles in cellular immune responses against HCV. The aim of the study was to evaluate the immunogenicity of the different HCV NS genes through transduction of DCs and presentation to T cells. Monocyte-derived DCs from healthy donors were infected with the recombinant adenovirus (Ad) harboring HCV NS3 (AdNS3), NS4 (NS4A and NS4B; AdNS4), NS5 (NS5A and NS5B; AdNS5), NS3/NS4 (AdNS3/NS4), and NS4/NS5 (AdNS4/NS5) genes, and then used to stimulate autologous lymphocytes in vitro.

[The distribution of hepatitis C virus genotype 1a in Yanbian area].

Objective: To investigate the distribution of Hepatitis C Virus (HCV)genotype 1a in Yanbian area, Jilin province.

Methods: The genotypes of the serum specimens of 47 patients with HCV from Yanbian area were determined by Scheme ABC of RFLP based on HCV 5' noncoding region (5'NCR). The samples of type 1a (Y2, Y4, Y6, and Y8) were amplified from the 5'NCR and NS5B regions by RT-PCR, and then sequenced directly.

[Utilization of Uracil-DNA glycosylase for combining reverse transcription and anti-contamination with polymerase chain reaction in hepatitis C virus].

Objective: To develop a hepatitis C virus(HCV) reverse transcription-polymerase chain reaction (RT-PCR) assay using Uracil-DNA glycosylase (UDG) for amplicon contamination control and evaluate the temperature and UDG concentrations for anti-contamination.

Methods: In this new HCV RT-PCR assay, reverse transcription, UDG anti-contamination and the first PCR were carried out at the same time. The layer candles were used to prevent the contamination in the second PCR.

[Analysis and state of HCV genotype 6a infection].

Objective: To investigate the infection state of hepatitis C virus genotype 6a in China.

Methods: Three (95, 126, 150)HCV genotype 6a serum samples were identified by digesting 5'NCR with compound enzyme method. Then, HCV 5'NCR and NS5B fragments were amplified from these samples by RT-PCR assay and sequenced.

[The difference in distribution of HCV genotypes between patients infected with HCV by transfusion and non-transfusion routes].

Objective: To investigate the HCV genotypes distribution in northern and southern cities in China and the difference between patients infected with HCV by transfusion and non-transfusion routes.

Methods: The HCV genotypes of the patients with chronic hepatitis C from 9 cities belonging to different regions were genotyped by the PCR products of 5 prime untranslated region NTR digested with restriction endonucleases, and the HCV genotypes distribution among different cities or between the patients infected with HCV through transfusion and other routes was analyzed.

Results: The HCV genotypes of 214 in 219 cases were determined; 197 patients were infected with monogenotype HCV.

[Hepatitis c virus genotype research by ABC programs of 5'-NCR restriction endonuclease digestion].

Objective: In order to fully understand hepatitis c virus (HCV) genotype 3b, 1a, 2b and 6a infection in China, We built HCV 5'-noncoding region (5'-NCR) of different genotypes and subtypes.

Methods: The classification HCV into variable genotypes (subtypes) was carried on by programs A, B and C A. Using a combination of three restriction endonuclease BHH' (BsrB I, Hae II, Hinf I) digestions at the same time.