[Molecular mechanisms of low intensity pulsed ultrasound-mediated cellular behavior in human primary macrophages].

The aim of this study was to explore the molecular mechanisms of the effect of low intensity pulsed ultrasound (LIPUS) on human primary macrophage functions. Macrophage phagocytosis was analyzed using fluorescein isothiocyanate (FITC)-labelled Escherichia coli (E.Coli); focal complex and extracellular matrix metalloproteinase inducer (EMMPRIN) were observed by fluorescence microscopy; the secretion of metalloproteinases (MMPs) was examined by gelatin zymography, and the expressions of EMMPRIN and extracellular signal-regulated kinases (ERKs) were detected by Western blot.

[Effect of LIPUS on the cellular behavior of human primary macrophages].

Aim: To explore the effect of low intensity pulsed ultrasound (LIPUS) on the biological behavior of macrophages to provide some theoretical foundations for its clinical application.

Methods: Human primary macrophages were obtained by using modified Ficoll-paque density gradient centrifugation combined with adherent method. The phagocytosis of macrophages was analyzed using fluorescein-labelled E.

Identification of quantitative trait loci controlling drought tolerance at seedling stage in Chinese Dongxiang common wild rice (Oryza rufipogon Griff.).

Common wild rice (Oryza rufipogon Griff.) is the ancestor of cultivated rice (O. sativa L.

[Effect of sustained inflation on inflammatory response of extra pulmonary organs in acute respiratory distress syndrome in rabbit].

Objective: To evaluate the influence of sustained inflation (SI) on inflammatory response of extra pulmonary organs in acute respiratory distress syndrome (ARDS) in rabbit.

Methods: The ARDS rabbit model were replicated by saline alveoli-lavage. The rabbits were randomly divided into four groups: 1.

[A comparison study on quantitative methods for the recruited volume in sheep with acute respiratory distress syndrome].

Objective: To compare equal pressure method with pressure-volume curve method to quantify the recruited volume.

Methods: Acute respiratory distress syndrome sheep model was induced by intravenous infusion 3 microg/kg lipopolysaccharide (LPS). Recruited volume of three different levels of positive end-expiratory pressure (PEEP=5, 10, 15 cm H2O) were measured both by pressure-volume curve method and by equal pressure method.

[Effects of positive end-expiratory pressure on lung recruited volume and oxygenation in patients with acute respiratory distress syndrome].

Objective: To access alveolar recruitment with positive end-expiratory pressure (PEEP) and to correlate the recruited volume with arterial oxygenation in acute respiratory distress syndrome (ARDS).

Methods: Eleven ventilated patients satisfying criteria of ARDS were included in the study group. Recruited volume of three different PEEP levels [5 cm H2O, 10 cm H2O, 15 cm H2O (1 cm H2O=0.

[Effect of lung protective ventilation strategy on pulmonary inflammatory response in a rabbit model of acute respiratory distress syndrome].

Objective: To evaluate the effect of lung protective ventilation strategy on pulmonary inflammatory response in acute respiratory distress syndrome (ARDS).

Methods: The ARDS rabbit model was duplicated by saline alveolar-lavage. The rabbits were divided into six groups: (1) normal control group (N group); (2) ARDS group (M group); (3) low-volume with best end-expiratory pressure (PEEP, A group) group: tidal volume (V(T)) 6 ml/kg, PEEP 2 cm H(2)O greater than the pressure of lower inflection point in pressure-volume curve (P(LIP)); (4) normal-volume with best PEEP group (B group): V(T) 6 ml/kg, and PEEP P(LIP) + 2 cm H(2)O; (5) low-volume with high PEEP group (C group): V(T) 6 ml/kg, and PEEP 15 cm H(2)O; and (6) high-volume with zero PEEP group (D group): V(T) 20 ml/kg.

[Comparison of effect of norepinephrine-dobutamine and that of dopamine alone on splanchnic perfusion in sheep with septic shock].

Objective: To compare the effect of norepinephrine-dobutamine and that of dopamine alone on splanchnic perfusion in sheep with septic shock.

Methods: Twenty sheep with septic shock induced by lipopolysaccharides were divided into two groups. When systolic pressure was decreased to 39.

Effects of norepinephrine, epinephrine, and norepinephrine-dobutamine on systemic and gastric mucosal oxygenation in septic shock.

Aim: To compare the effects of dopamine, norepinephrine, epinephrine, and the combination of norepinephrine and dobutamine on systemic and gastric mucosal oxygen metabolism in patients with septic shock.

Methods: Sixteen patients with septic shock were enrolled in the present study. Each patient received dopamine firstly, then in a random succession epinephrine, norepinephrine, or norepinephrine-dobutamine, a mean systemic arterial pressure adjusted to >9.

Comparison of norepinephrine-dobutamine to dopamine alone for splanchnic perfusion in sheep with septic shock.

Aim: To compare the effect of norepinephrine-dobutamine with dopamine alone on splanchnic perfusion in sheep with septic shock.

Methods: Twenty sheep with septic shock induced by lipopolysaccharides were divided into two groups. When systolic pressure decreased by 5.