circXRCC5 foster gastric cancer growth and metastasis by the HNRNPC/circXRCC5/miR-655-3p/RREB1/UBA2 positive feedback loop.

Gastric cancer (GC) is one of the most common malignancies, leading to millions of deaths each year. Here, we investigated the molecular mechanisms of GC, with a focus on circXRCC5/miR-655-3p/RREB1/UBA2 axis. circXRCC5 was identified in 62 paired cancer specimens and adjacent normal tissues by genome-wide bioinformatics analysis and verified by qRT-PCR and Sanger sequencing.

Comparative effects of pollen limitation, floral traits and pollinators on reproductive success of Hedysarum scoparium Fisch. et Mey. in different habitats.

Background: Reproduction in most flowering plants may be limited because of the decreased visitation or activity of pollinators in fragmented habitats. Hedysarum scoparium Fisch. et Mey.

Inoculation With Indigenous Rhizosphere Microbes Enhances Aboveground Accumulation of Lead in Thunb. by Improving Transport Coefficients.

The application of plant-microbial remediation of heavy metals is restricted by the difficulty of exogenous microbes to form large populations and maintain their long-term remediation efficiency. We therefore investigated the effects of inoculation with indigenous heavy-metal-tolerant rhizosphere microbes on phytoremediation of lead (Pb) by We measured plant physiological indexes and soil Pb bioavailability and conducted widespread targeted metabolome analysis of strains to better understand the mechanisms of enhance Pb accumulation. Growth of was improved by both single and co-inoculation treatments with sp.

Upregulation of tumor necrosis factor-alpha in the anterior cingulate cortex contributes to neuropathic pain and pain-associated aversion.

Injury associated pain involves subjective perception and emotional experience. The anterior cingulate cortex (ACC) is a key area involved in the affective component of pain processing. However, the neuroimmune mechanisms underlying enhanced ACC excitability following peripheral nerve injury are still not fully understood.

Calpain-2 Regulates TNF-α Expression Associated with Neuropathic Pain Following Motor Nerve Injury.

Both calpain-2 (CALP2) and tumor necrosis factor-α (TNF-α) contribute to persistent bilateral hypersensitivity in animals subjected to L5 ventral root transection (L5-VRT), a model of selective motor fiber injury without sensory nerve damage. However, specific upstream mechanisms regulating TNF-α overexpression and possible relationships linking CALP2 and TNF-α have not yet been investigated in this model. We examined changes in CALP2 and TNF-α protein levels and alterations in bilateral mechanical threshold within 24 h following L5-VRT model injury.

Early CALP2 expression and microglial activation are potential inducers of spinal IL-6 up-regulation and bilateral pain following motor nerve injury.

Previous work from our laboratory showed that motor nerve injury by lumbar 5 ventral root transection (L5-VRT) led to interleukin-6 (IL-6) over-expression in bilateral spinal cord, and that intrathecal administration of IL-6 neutralizing antibody delayed the induction of mechanical allodynia in bilateral hind paws. However, early events and upstream mechanisms underlying spinal IL-6 expression following L5-VRT require elucidation. The model of L5-VRT was used to induce neuropathic pain, which was assessed with von Frey hairs and the plantar tester in adult male Sprague-Dawley rats.

Bulleyaconitine A preferably reduces tetrodotoxin-sensitive sodium current in uninjured dorsal root ganglion neurons of neuropathic rats probably via inhibition of protein kinase C.

Oral Bulleyaconitine A (BLA) is effective for treating neuropathic pain in human patients, but the underlying mechanism is poorly understood. Here, we tested whether BLA blocked voltage-gated sodium channels (VGSCs) in dorsal root ganglion (DRG) neurons. Compelling evidence shows that voltage-gated sodium channels are upregulated in uninjured DRG neurons but downregulated in injured ones following peripheral nerve injury.

Effects of sand burial on the survival and growth of two shrubs dominant in different habitats of northern China.

Plants that grow in dune ecosystems always suffer from sand burial. Shrubs play implications on the healthy functioning of dune ecosystems due to control blowing sand. However, the survival and growth responses of shrubs to sand burial remain poorly understood.

[Immunophenotypic Analysis of Acute Promyelocytic Leukemia].

Objective: To investigate the immunophenotype of leukemia promyelocytes (LP) in bone marrow of patients with acute promyelocytic leukemia (APL) and to explore their characteristics and significance.

Methods: The immunophenotypes of leukemia cells in 43 patients with APL were analyzed by means of 4 color immunophenotypes; the cell population in which CD45 strength localized at 10(2) and the SSC strength locatized at 10(2) was defined as R3, the cell population in which CD45 strength localized at 10(3) and the SSC strength localized at 10(2) was defined as R5, moreover the ratio of positive cells >80% was defined as strong positive expression, the ratio of positive cells between 20%-80% was difined as weak positive expression, the ratio of positive cells <20% was difined as negative by gating method of CD45/SSC.

Results: There was a abnormal cell population (R3) in 79.

Effect of vasopressin on hippocampal injury in a rodent model of asphyxial cardiopulmonary arrest.

The effect of vasopressin on the neuronal injury following the restoration of spontaneous circulation (ROSC) in cardiac arrest (CA) is not yet fully understood. The present study was conducted in order to investigate the effect of vasopressin alone, or in combination with epinephrine, on the ROSC and hippocampal injury in a rat model of asphyxial CA. Asphyxial CA was induced in 144 rats by clamping the tracheal tube, and animals were allocated equally into the following three groups: Treatment with vasopressin (0.

Responses of soil fungal community to the sandy grassland restoration in Horqin Sandy Land, northern China.

Sandy grassland restoration is a vital process including re-structure of soils, restoration of vegetation, and soil functioning in arid and semi-arid regions. Soil fungal community is a complex and critical component of soil functioning and ecological balance due to its roles in organic matter decomposition and nutrient cycling following sandy grassland restoration. In this study, soil fungal community and its relationship with environmental factors were examined along a habitat gradient of sandy grassland restoration: mobile dunes (MD), semi-fixed dunes (SFD), fixed dunes (FD), and grassland (G).

Optimization extraction process of aroma components in tobacco.

This paper concerns the optimization of the simultaneous distillation extraction process of volatile aromatic components in flue-cured tobacco leaves by single-factor experiments and response surface methodology (RSM). The qualitative and quantitative analysis of the aroma components was performed by gas chromatography-mass spectrometry. The single-factor experiments were adopted to investigate the effects of five independent variables (including material/liquid ratio, distillation time, dosage of NaCl, volume of CH(2)Cl(2) and water-bath temperature) on the extraction of aroma components in tobacco.

[Dynamic changes of surface soil organic carbon and light-fraction organic carbon after mobile dune afforestation with Mongolian pine in Horqin Sandy Land].

This paper studied the dynamic changes of surface (0-15 cm) soil organic carbon (SOC) and light-fraction organic carbon (LFOC) in 25- and 35-year-old sand-fixing Mongolian pine (Pinus sylvestris var. mongolica) plantations in Horqin Sandy Land, with a mobile dune as a comparison site. After the afforestation on mobile dune, the content of coarse sand in soil decreased, while that of fine sand and clay-silt increased significantly.

[Challenge to the desertification reversion in Horqin Sandy Land].

Analyses of the changes in desertified land area, water resource availability, land use, and plant productivity in Horqin Sandy Land in recent 50 years showed that from 1950 to the late 1980s, the land desertification in Horqin Sandy Land had a rapid expansion, but reversed since then. The annual runoff of Xiliaohe River decreased consistently, and in 1999, the middle reach at Tongliao section was dried up. In recent 20 years, the water table of Xihu Lake was decreased by about 10 m, and dried up in 2001.

[Crust development and subsurface soil properties under dominant shrubs in the process of dune restoration, Horqin Sand Land].

Soil crust is a common and widespread phenomenon in desert areas all over the world due to its extraordinary ability to survive desiccation and extreme temperatures, high pH and salinity. Despite its unassuming appearance, biological soil crusts play a significant role in desert ecosystems, including involvement in the process of formation, stability and fertility of soil, preventing soil erosion by water or wind, increasing the possibility of vascular plant colonization, and being responsible for the stabilization of sand dunes. This study taking Horqin Sand Land as research region, by field sampling, crust and topsoil (0-2.

Free and conjugated phytosterols in cured tobacco leaves: influence of genotype, growing region, and stalk position.

Although phytosterols in tobacco leaves have specific effects on tobacco quality, there is little research on the distribution of free and conjugated phytosterols in various tobacco leaves. In this paper, we explored the content and composition of phytosterols in representative cured tobacco leaves by gas chromatography of TMS-ether derivatives. We found that phytosterol contents in tobacco leaves ranged from 1.