Investigating the molecular mechanisms of oocyte maturation and ovulation in Nile tilapia: A focus on the steroidogenic enzyme Cyp17a2.

Previous research has highlighted the significant role of progestins and glucocorticoids in fish oocyte maturation and ovulation. To clarify the molecular mechanisms underlying these processes, comprehensive investigations were conducted using a mutant Nile tilapia ( ) model. Analysis revealed pronounced Cyp17a2 expression in ovarian somatic cells of the tilapia.

[Clinical study of isthmic spondylolisthesis treated with Mis-TLIF assisted by preoperative posture reduction and intraoperative lifting reduction].

Objective: To investigate the clinical effect of minimally invasive transforaminal lumbar interbody fusion(Mis-TLIF) technique combined with preoperative position reduction in the treatment of spondylolisthesis and summarize its advantages.

Methods: Between July 2016 and July 2022, 60 patients with lumbar isthmic spondylolisthesis were retrospectively analyzed, including 26 males and 34 females, with an average age of (51.32±4.

Intraoperative changes of surgical approach and a second surgery after percutaneous endoscopic surgery for lumbar spinal stenosis.

Objective: To investigate the reasons for a second surgery after Percutaneous Endoscopic Surgery (PES) for lumbar spinal stenosis and to provide references for the choice of indications and appropriate surgical approach.

Method: A total of 426 patients received PES for lumbar spinal stenosis. The postoperative, intraoperative, and postoperative data of the subjects were analyzed.

[Effects of tramadol hydrochloride preemptive analgesia in kyphoplasty of thoracolumbar osteoporotic fractures under local anesthesia].

Objective: To explore preemptive analgesic effect of preoperative intramural tramadol injection in percutaneous kyphoplasty (PKP) of vertebrae following local anesthesia.

Methods: From August 2019 to June 2021, 118 patients with thoraco lumbar osteoporotic fractures were treated and divided into observation group and control group, with 59 patients in each gruop. In observation group, there were 26 males and 33 females, aged from 57 to 80 years old with an average of (67.

[Stable isotopes of zooplankton and their applications in the research of aquatic ecosystems].

Zooplankton plays a mediating role in the food web of aquatic ecosystems, the stable carbon and nitrogen isotopes (δC and δN) of which have been widely used to study the utilization of food resources, material cycling pathways, and trophic relationships. The δC and δN values of zooplankton have been used to predict primary productivity, sources and sinks of pollutants and environmental changes. To better use δC and δN of zooplankton as ecological and environmental indicators, it is particularly important to understand their temporal and spatial variations and the influencing factors.

Expression and clinical significance of periostin in oral lichen planus.

Oral lichen planus (OLP) is a chronic inflammatory lesion involving the oral mucosa, which has a high likelihood of progressing to cancer. The present study investigated the periostin expression in the mucosa and serum of patients with OLP and its correlation with serum cytokines. A total of 117 patients with OLP and 110 healthy controls were included in the study.

[Mini-open trans-spatium intermuscular versus percutaneous short-segment pedicle fixation for the treatment of thoracolumbar mono-segmental vertebral fractures].

Objective: To compare the clinical effects and radiographic outcomes of mini-open trans-spatium intermuscular and percutaneous short-segment pedicle fixation in treating thoracolumbar mono-segmental vertebral fractures without neurological deficits.

Methods: From August 2009 and August 2012, 95 patients with thoracolumbar mono-segmental vertebral fractures without neurological deficits were treated with short-segment pedicle fixation through mini-open trans-spatium intermuscular or percutaneous approach. There were 65 males and 30 females, aged from 16 to 60 years old with an average of 42 years.

[Treatment of flexion-distraction thoracolumbar fractures by postural reduction with instrumental reduction].

Objective: To study the curative effect of postural reduction with instrumental reduction in treatment of flexion-distraction thoracolumbar fractures.

Methods: A retrospective study was performed on 43 patients with single thoracolumbar flexion-distraction fractures admitted from August 2009 to August 2011, included 28 males and 15 females with an average age of 44 years old (34 to 56 years old). All patients were treated with postural reduction with instrumental reduction.

[Analysis of epidural hematoma formative reason and its preventive measure after anterior cervical operation].

Objective: To explore the risk factors,preventive measure of epidural hematoma after anterior cervical operation.

Methods: From June 2005 and December 2012, 1,452 patients underwent anterior cervical operation in our hospital. Epidural hematoma occurred in 5 cases after operation and the incidence rate was 0.

[Transoral plate internal fixation for treatment of instability atlas fracture].

Objective: To evaluate the efficacy and safety of transoral plate internal fixation for instability atlas fracture.

Methods: A retrospective study was performed in eight patients with instability atlas fractures, who were treated by a transoral plate internal fixation from July 2007 to June 2011. There were 6 males and 2 females,with an average age of 39.

[Clinical application of lower cervical spinous process laminar screw technique in open door laminoplasty].

Objective: To investigate the clinical outcomes of lower cervical spinous process laminar screw technique in open door laminoplasty.

Methods: From February 2005 to June 2010,12 patients with cervical myelopathy were treated with open door laminoplasty by lower cervical spinous process laminar screw technique. There was intervertebral disc herniation with degenerative stenosis in 5 patients, ossification of posterior longitudinal ligament with osteophyte in 6 patients, cervical traumatic instability with spinal cord injuries in 1 patient.

[Anatomic study of lower cervical spinous process for laminar screw fixation].

Objective: To measure anatomic data of spinous process and vertebral plate and evaluate the possibility of fixation of spinous process and vertebral plate.

Methods: Twenty cadaveric cervibal spines of C3-C7 were dissected to expose vertebral plate and spinous process, and were measured by two methods, one was manual measured using digital caliper and the other was computed tomography scaning. Parameters were measured manually including vertebral plate height and width.

[Micro-endoscopic discectomy for the treatment of lumbar disc herniation in senile patients over seventy years old].

Objective: To explore applicability and therapeutic effects of Micro-endoscopic discectomy (MED) for the treatment of lumbar disc herniation in senile patients over seventy years old.

Methods: Thirty-two patients over seventy years old with lumber disc herniation were treated by MED from December 2007 to June 2010. Among them,20 patients were male and 12 patients were female,ranging in age from 70 to 86 years, with an average of 78.

[Anatomic comparison between spinous process screws and pedicle screws techniques of the second cervical vertebar].

Objective: To compare anatomic difference between spinous process screws and pedicle screws techniques of the second cervical vertebra.

Methods: Ten human cadaveric of cervical spine (5 male, 5 female) were harvested and had no gross deformities such as scoliosis and/or kyphosis were found in the study. The average age of the subjects was 60.

NF-κB induces abnormal centrosome amplification by upregulation of CDK2 in laryngeal squamous cell cancer.

Centrosome amplification can drive chromosomal instability (CIN) which is a major source of tumor initiation. The present study aimed to investigate the impact of nuclear factor kappa B (NF-κB) on centrosome amplification of Hep-2 cells. Immunofluorescence was performed to display centrosomes.

[Effect of exogenous adrenomedullin on renal and hypothalamus adrenomedullin expression in rats early after mechanical renal trauma].

Objective: To observe the effects of exogenous adrenomedullin (ADM) on endogenous expression of ADM in the kidney and hypothalamus of rats early after mechanical renal trauma.

Methods: Adult Wistar rats were randomized into 4 groups (n=32), namely the control group, renal impact trauma group, preventive ADM injection group, and therapeutic ADM injection group. In the latter two groups, ADM (0.

[An anatomic study of posterior C(2) spinous process laminar screw fixation].

Objectives: To explore the feasibility and the technical parameters in posterior C2 spinous process laminar screw fixation, and discuss the clinic significance of C2 spinous process laminar screws.

Methods: Twenty cervical cadaveric spines of C2 were dissected, with care taken to expose the lamina and spinous process. After the entrance point of spinous process screw were determined, posterior C2 spinous process laminar screw implantation was performed under visual control.

[Anatomical considerations for the placement of C₂ spinous process screws].

Objectives: To quantitatively anatomically evaluate the C₂ spinous process, analyze the anatomical feasibility of the C₂ spinous process screws and its clinical significance.

Methods: To dissect and evaluate 30 cervical cadaveric spines of C₂ which were taken to expose the lamina and spinous process. Anatomic quantitative evaluation of the C₂ spinous process included its height and width.

[Clinical observation of mid-stage complications after cervical disc replacement].

Objective: To summarize the therapeutic experience of Bryan prosthetic cervical disc replacement at the 3rd years after operation and to analyze the clinical effect, incidence rate of heterotopic ossification, conditions of prosthetic fusion in order to investigate the countermeasures.

Methods: From December 2005 to December 2008, 54 patients with cervical syndrome (34 males and 20 females, the age was from 39 to 69 years with an average of 50.5) were treated with single level Bryan prosthetic cervical disc replacement.

[Biomechanical study on transarticular screw and plate fixation syetem in the lower cervical spine].

Objective: To compare the cervical stabilities of transarticular screw fixation alone, and transarticular screw and plate fixation system.

Methods: Twelve specimens of cervical vertebra containing C2-T1 were obtained from human cadaver. The three-column instability model was made at C4.

[Experimentation and investigation of the effects of TNF and the acceptor expression in renal early trauma with extraneous adrenomedullin].

Objective: To investigate the effects of TNF-alpha, TNF-beta and the acceptor expression about mechanical renal trauma with extraneous ADM.

Methods: There were 104 healthy adult plain grade Wistar rat, randomly divided into four groups:8 in the group of control, 32 in the group of trauma, 32 in the group injected ADM before trauma, 32 in the group injected ADM post trauma. The experimental model of rat kidney with mechanical trauma was prepared by striking the area of rat skin reflecting by kidney with free dropping ferrous hammer in the last three groups.