Transfection of bone marrow mesenchymal stem cells using green fluorescence protein labeled hVEGF165 recombinant plasmid mediated by liposome.

Objective: To study the role of bone marrow mesenchymal stem cells (BMSCs) in construction of vascularized engineered tissue.

Methods: hVEGF165 was amplified via RT-PCR before recombinant with pShuttle- green fluorescence protein;green fluorescent protein (GFP)-CMV. Then the recombinant shuttle plasmid was transfected into BMSCs with Lipofectamine(TM) 2000 for packaging and amplifying.

Risk analysis for the highly pathogenic avian influenza in Mainland China using meta-modeling.

A logistic model was employed to correlate the outbreak of highly pathogenic avian influenza (HPAI) with related environmental factors and the migration of birds. Based on MODIS data of the normalized difference vegetation index, environmental factors were considered in generating a probability map with the aid of logistic regression. A Bayesian maximum entropy model was employed to explore the spatial and temporal correlations of HPAI incidence.

The novel H1N1 Influenza A global airline transmission and early warning without travel containments.

A novel influenza A (H1N1) has been spreading worldwide. Early studies implied that international air travels might be key cause of a severe potential pandemic without appropriate containments. In this study, early outbreaks in Mexico and some cities of United States were used to estimate the preliminary epidemic parameters by applying adjusted SEIR epidemiological model, indicating transmissibility infectivity of the virus.

Simulation of the spread of infectious diseases in a geographical environment.

The study of mathematical models for the spread of infectious diseases is an important issue in epidemiology. Given the fact that most existing models cannot comprehensively depict heterogeneities (e.g.