The effect of pretreatment with calcitonin gene-related peptide on attenuation of liver ischemia and reperfusion injury due to oxygen free radicals and apoptosis.

Background/aims: Hepatic ischemia reperfusion injury (I/R) occurs in the settings of transplantation. Trauma and elective liver resections. Reactive oxygen species (ROS) and apoptosis have been shown to play a major role in organ I/R injury.

Evaluation of rat liver apoptotic and necrotic cell death after cold storage using UW, HTK, and Celsior.

Background: The benefit of Celsior in liver graft preservation is controversial. In the isolated perfused rat liver model, we compared the effects of Celsior, University of Wisconsin (UW), and histidine-tryptophan-ketoglutarate (HTK) preservation solutions on liver cell death.

Methods: Rat livers were stored at 4 degrees C for 0, 8, 16, or 24 hr in either Celsior, UW, or HTK and reperfused for 90 min (37 degrees C).