Phylogenomics characterization of a highly virulent Edwardsiella strain ET080813(T) encoding two distinct T3SS and three T6SS gene clusters: Propose a novel species as Edwardsiella anguillarum sp. nov.

As important zoonotic organisms causing infections in humans, Edwardsiella bacteria are also notorious leading fish pathogens haunting worldwide aquaculture industries. However, the taxa are now widely recognized to be misclassified, which hurdled the understanding of the epidemiology and development of effective diagnostics and vaccines. Currently the genus Edwardsiella consists of three species Edwardsiella tarda, E.

Anti-inflammatory and anti-thrombogenic effects of atorvastatin in acute ischemic stroke.

Atorvastatin decreases inflammation and thrombogenesis in patients with carotid artery plaque. Atorvastatin is administered to lower lipid levels, but its anti-inflammatory and anti-thrombogenic effects remain unclear. Eighty-nine patients from northeastern China with acute ischemic stroke caused by large-artery atherosclerosis were randomly divided into the study and control groups.

High gene delivery efficiency of alkylated low-molecular-weight polyethylenimine through gemini surfactant-like effect.

To our knowledge, the mechanism underlying the high transfection efficiency of alkylated low-molecular-weight polyethylenimine (PEI) is not yet well understood. In this work, we grafted branched PEI (molecular weight of 1,800 Da; bPEI1800) with lauryl chains (C₁₂), and found that bPEI1800-C₁₂ was structurally similar to gemini surfactant and could similarly assemble into micelle-like particles. Stability, cellular uptake, and lysosome escape ability of bPEI1800-C₁₂/DNA polyplexes were all greatly enhanced after C₁₂ grafting.

(99m)Tc-labeled porphyrin-lipid nanovesicles.

Porphyrin-lipid nanovesicles (PLN) have been developed with intrinsic capabilities as activatable multimodal photonic contrast agents. Radiolabeling of PLN encapsulating drugs could eventually be able to provide quantitative in vivo information for diagnosing and treating diseases. In this study, we developed (99m)Tc-labeled porphyrin-lipid nanovesicles ((99m)Tc-PLN) as a cargo-encapsulated formulation without significant impact on liposome integrity and encapsulation stability.

Small-sized polymeric micelles incorporating docetaxel suppress distant metastases in the clinically-relevant 4T1 mouse breast cancer model.

Background: The small size of ultra-small nanoparticles makes them suitable for lymphatic delivery, and many recent studies have examined their role in anti-metastasis therapy. However, the anti-metastatic efficacy of small-sized nanocarriers loaded with taxanes such as docetaxel has not yet been investigated in malignant breast cancer.

Methods: We encapsulated docetaxel using poly(D,L-lactide)1300-b-(polyethylene glycol-methoxy)2000 (mPEG2000-b-PDLLA1300) to construct polymeric micelles with a mean diameter of 16.

Robustness of a partially interdependent network formed of clustered networks.

Clustering, or transitivity, a behavior observed in real-world networks, affects network structure and function. This property has been studied extensively, but most of this research has been limited to clustering in single networks. The effect of clustering on the robustness of coupled networks, on the other hand, has received much less attention.

Porphyrin-phospholipid liposomes permeabilized by near-infrared light.

The delivery of therapeutic compounds to target tissues is a central challenge in treating disease. Externally controlled drug release systems hold potential to selectively enhance localized delivery. Here we describe liposomes doped with porphyrin-phospholipid that are permeabilized directly by near-infrared light.

Spiral patterns of dislocations at nodes in (111) semi-coherent FCC interfaces.

In semi-coherent interface, a superposed network of interface dislocations accommodates the attendant coherency strains in the adjacent crystals and their intersections (referred to as nodes) can act as sinks and sources for point defects because of the low formation energy. Nodes in {111} semi-coherent interfaces are characterized with a spiral pattern (SP), wherein the line direction of each dislocation entering a node curves. The structure of SP nodes is able to switch between condensed and expanded by either reaction with point defects or mechanical deformation.

Radioprotective effect of adenine on irradiation-induced apoptosis.

Aim: To investigate the radioprotective effect of adenine on irradiated lymphocytes and discover the possible mechanisms of protection.

Methods: Lymphocytes were pretreated for 12 h with adenine (0.001-0.

Comparative proteomic analysis reveals new components of the PhoP regulon and highlights a role for PhoP in the regulation of genes encoding the F1F0 ATP synthase in Edwardsiella tarda.

Edwardsiella tarda is an important cause of haemorrhagic septicaemia in fish and also of gastro- and extra-intestinal infections in humans. We have recently demonstrated that the PhoP-PhoQ two-component regulatory system plays important roles in both virulence and stress tolerance in E. tarda.

[Flavonoids of Erigeron canadensis].

Objective: To study the flavonoids of Erigeron canadensis.

Method: The constituents of EtOH extraction from the whole plant of E. canadensis were isolated and purified by repeated column chromatography.

Influence of neoadjuvant chemotherapy on HER2/neu status in invasive breast cancer.

Introduction: Reliably estimating HER2/neu expression in breast cancer is important for predicting patient prognosis and optimizing adjuvant therapeutic strategies. In this retrospective cohort study, effects of NAC on HER2/neu status in invasive breast cancer were evaluated, and the related factors were analyzed.

Patients And Methods: One hundred thirty-one patients with primary breast cancer were treated with anthracycline- and/or taxane-based NAC.

Factors affecting clot lysis rates in patients with spontaneous intraventricular hemorrhage.

Background And Purpose: In the treatment of severe intraventricular hemorrhage (IVH), thrombolytic use and clot size are known to influence clot lysis rates. We evaluated the effect of other variables on IVH clot lysis rates among patients treated with recombinant tissue-type plasminogen activator or placebo.

Methods: One hundred patients with IVH and intracerebral hemorrhage volume <30 mL requiring emergency external ventricular drainage from 2 multicenter trials were treated with intraventricular administration of recombinant tissue-type plasminogen activator (n=78; 53 males, 25 females) or placebo (n=22; 7 males, 15 females).

catena-[[(nitrato-κO)silver(I)]-μ-1,10-phenanthroline-5,6-dione-κO,O':N,N'].

In the title one-dimensional coordination polymer, [Ag(NO(3))(C(12)H(6)N(2)O(2))](n), the Ag(I) atom is penta-coordinated by two N atoms from a 1,10-phenanthroline-5,6-dione (phen-dione) ligand, one O atom from the nitrate anion and two O atoms from another phen-dione ligand. The coordination environment around silver is slightly distorted square-pyramidal. Inter-estingly, the Ag-O distances to the phen-dione ligand are different [Ag-O = 2.

[Pharmacognostical identification of the leaf of Uncaria hirsuta Havil].

Objective: To perform a pharmacognostical study of the leaf of Uncaria hirsuta Havil.

Methods: The specimens of Folium Uncariae Hirsutae were collected for studying its characteristics, microscopic appearance and thin-layer chromatography.

Results: The leaf of Uncaria hirsuta Havil was characterized by numerous multicellular non-glandular hairs, 2 lines of palisade tissue, a diacytic type of stoma, and clustered crystals in its parenchyma.

[Studies on the constituents from the herba of Erigeron acer].

Objective: To investigate the chemical constituents of the aerial part of Erigeron acer.

Methods: The chemical constituents were isolated by various columns and thin layer chromatographic methods. The structures were identified by spectral data.

2-Chloro-N,N-diphenyl-acetamide.

In the title compound, C(14)H(12)ClNO, the central acetamide plane forms dihedral angles of 76.0 (2) and 64.0 (2)° with the phenyl rings and the phenyl rings form a dihedral angle of 71.

N-(4-Chloro-phenyl)-1,8-naphthalimide.

In the title compound, C(18)H(10)ClNO(2), the naphthalimide ring system is almost planar, the rings forming dihedral angles of 2.05 (3), 2.26 (3) and 0.