[Impacts of establishment of chest pain center on the door-to-balloon time and the short-term outcome after primary percutaneous coronary intervention of patients with ST segment elevated myocardial infarction].

Objective: To investigate the impact of the establishment of chest pain center (CPC) model based on the pre-hospital real-time tele-12-lead electrocardiogram on the door-to-balloon (D-to-B) time and short-term outcome after primary percutaneous coronary intervention (PPCI) of patients with ST-segment elevated myocardial infarction (STEMI).

Methods: A regular CPC was established with pre-hospital transmitted real-time 12-lead electrocardiogram system for pre-hospital diagnosis of STEMI and enabled the STEMI patients to bypass the emergency room and directly treated in the catheter lab to shorten the D-to-B time. The mean D-to-B time, the short-term outcome and medical costs were compared in PPCI patients before (93 cases, group A) and after (149 cases, group B) the establishment of CPC.

[The clinical application of the emergency remedial transthoracic cardiac pacing used by a steel wire loop electrode].

Objective: To explore an effective method of emergency remedial cardiac pacing and evaluate its clinical application.

Methods: The transthoracic cardiac pacing was used by a steel wire loop electrode to 18 patients with cardiac arrest or serious bradycardia, after a routine cardiac pacing was failed by way of jugular or subclavian vein.

Results: It is a simple procedure used by steel wire loop.

[Clinical features of idiopathic ventricular tachycardia of various types and their radiofrequency ablation therapy].

Objective: To analyze the clinical features idiopathic ventricular tachycardia (IVT) and evaluate the effect of radiofrequency ablation therapy for their management.

Methods: An retrospective analysis was conducted in 165 IVT patients who received radiofrequency ablation therapy. IVT was classified into 3 types according to the site of origin, namely the right ventricular outflow tract (RVOT-IVT, 86 cases), left ventricular septum (LV-IVT, 75 cases), and left Valsalva sinus (4 cases).

[Fever and Brugada syndrome: report of 21 cases].

A retrospective analysis of 21 cases of Brugada syndrome treated between 1997 and 2004 was conducted to examine the clinical characteristics of these patients and the relations between fever and Brugada syndrome. Of the 21 patients including one female patient, 4 male patents with Brugada syndrome were confirmed to develop ventricular arrhythmias due to febrile disease, suggesting that fever, one of the common causes for triggering cardiac events in Brugada syndrome, should receive due attention in clinical practice.

[PCR-based site-directed mutagenesis and recombinant expression plasmid construction of a SCN5A mutation (K317N) identified in a Chinese family with Brugada syndrome].

Objective: To perform PCR-based site-directed mutagenesis of a new SCN5A mutation (K317N) identified in a Chinese family with Brugada syndrome and construct the recombinant expression plasmid pRc/CMV-Hh1 containing the human cardiac sodium channel alpha subunit (hH1), mutant cDNA.

Methods: A pair of primers was designed according to the restricted sites Sse 8387I and Age I of the SCN5A sequence with the mismatches introduced into primers. Mutagenesis was performed in a single-step PCR, and the fragments amplified by PCR containing the mutation site were subcloned into the pRc/CMV-hH1 vector.