Single Nucleotide Recognition and Mutation Site Sequencing Based on a Barcode Assay and Rolling Circle Amplification.

Single nucleotide polymorphisms (SNPs) present significant challenges in microbial detection and treatment, further raising the demands on sequencing technologies. In response to these challenges, we have developed a novel barcode-based approach for highly sensitive single nucleotide recognition. This method leverages a dual-head folded complementary template probe in conjunction with DNA ligase to specifically identify the target base.

Construction of a zero-dimensional halide perovskite in micron scale towards a deeper understanding of phase transformation mechanism and fluorescence applications.

Zero-dimensional (0D) halide perovskites have garnered significant interest due to their novel properties in optoelectronic and energy applications. However, the mechanisms underlying their phase transformations and fluorescence properties remain poorly understood. In this study, we have synthesized a micron-scale 0D perovskite observable under confocal laser scanning microscopy (CLSM).

Perovskite-based electrochemiluminescence analysis of HO.

The detection of hydrogen peroxide (HO) represents an extensive requirement across various domains, including food, environmental, and medical fields. This study introduces a highly sensitive technique for the quantification of HO, integrating the electrochemiluminescence properties of perovskite with bio-catalyzed precipitation. A water-soluble perovskite-based electrochemiluminescence (ECL) biosensing interface was constructed, wherein HO catalyzes a precipitation reaction that leads to the formation of an insoluble precipitate on the electrode surface.

A perovskite-based electrochemiluminescence aptasensor for tetracycline screening.

Tetracyclines are currently the most commonly used class of antibiotics, and their residue issue significantly impacts public health safety. In this study, a surface modification of perovskite with cetyltrimethylammonium bromide led to the generation of stable electrochemiluminescence (ECL) emitters in aqueous systems and improved the biocompatibility of perovskite. A perovskite quantum dot-based ECL sensing strategy was developed.

An extracellular electron transfer enhanced electrochemiluminescence aptasensor for analysis.

As a crucial indicator in food and water safety testing, the detection of plays a significant role in maintaining environmental sanitation and promoting public health. Herein, based on the electrochemical activity characteristics of , we established an enhanced electrochemiluminescence aptasensor for analysis. This study presents a new method for accurate identification by utilizing a double aptamer recognition system.

Target-cycling synchronized rolling circle amplification strategy for biosensing Helicobacter pylori DNA.

Helicobacter pylori is closely linked to many gastric diseases such as gastric ulcers and duodenal ulcers. Therefore, biosensing H. pylori has attracted wide attention from both scientists and clinicians.

A CRISPR/Cas12a-assisted array for Helicobacter pylori DNA analysis in saliva.

Helicobacter pylori infection has become a threat to the world populations. This leads to an urgent need of an efficient and convenient approach to accurately diagnose H. pylori infection.

Identification of multiple single-nucleotide variants for clinical evaluation of Helicobacter pylori drug resistance.

Helicobacter pylori infections are threats to public health due to their high infection rate and drug resistance. Identification of single-nucleotide variants (SNVs) in H. pylori is crucial for both diagnosis and therapy.

A portable electrochemiluminescence bipolar electrode array for the visualized sensing of Cas9 activity.

CRISPR/Cas9 has become a powerful tool for genomic manipulation, and the evaluation of the Cas9 activity is essential for the precise control of the CRISPR system. Herein, we develop a point-of-care platform for the rapid and visible determination of the Cas9 activity. A bipolar electrochemiluminescence (ECL) biosensor platform comprises an Au electric circuit fabricated on a glass substrate and the PDMS layer acts as a reservoir.

Targeted Transmembrane Delivery of Ca via FA-Nanogel for Synergistically Enhanced Chemotherapy.

Metal ion synergistically enhanced chemotherapy is a promising strategy for cancer treatment. However, targeting delivery of ions toward cancer cells remains challenging for decades. Herein, we developed a novel folic acid-nanogel (termed as FA-nanogel) with alkane chains as diffusion barriers for targeted transmembrane delivery of calcium ions into cancer cells.

A simple FRET system using two-color CdTe quantum dots assisted by cetyltrimethylammonium bromide and its application to Hg(II) detection.

In this study, we developed a novel simple fluorescence resonance-energy transfer (FRET) system between two-color CdTe quantum dots (QDs) assisted by cetyltrimethylammonium bromide (CTAB). Mercaptopropionic (MPA)-capped CdTe QDs serving as both donors and acceptors were successfully synthesized by changing the refluxing time in aqueous solution. CTAB micelles formed in water and minimized the distance between the donors and acceptors significantly by electrostatic interactions, improving FRET efficiency.

An exploration of nucleic acid liquid biopsy using a glucose meter.

The development of non-invasive techniques for the diagnosis of cancer, characterization of mutation and monitoring treatment response could greatly reduce the morbidity and mortality caused by cancer. Nevertheless, the extremely low amount of cell free nucleic acids makes liquid biopsy a very challenging task. Herein, taking advantage of the pocket size, reliable quantitative results and simple operation of the pocket-sized personal glucose meter (PGM), we report an approach of circulating microRNA-21 (miR-21) detection with high precision and low cost.

Microfluidic liquid-air dual-gradient chip for synergic effect bio-evaluation of air pollutant.

In this paper, a novel prototype liquid-air dual gradient chip is introduced, which has paved the way for effective synergic effect bio-evaluation of air pollutant. The chip is composed of an array of the agarose liquid-air interfaces, top air gradient layer and bottom liquid gradient layer. The novel agarose liquid-air interface allows for non-biased exposure of cells to all the substances in the air and diffusive interactions with the liquid phase; while the dual liquid-air gradient provides powerful screening abilities, which well reduced errors, saved time and cost from repeated experiment.

A paper-based SERS test strip for quantitative detection of Mucin-1 in whole blood.

A paper-based SERS test strip combining strengths of paper chip and Raman active substrate was demonstrated to overcome challenges in spectroscopic sensing of complicated samples and realize quantitative detection of disease markers in whole blood. The precisely controlled Au NPs were not only capable of generating condensed hot spots on the fibers, but also enhanced the size exclusion effect of paper, resulting in the novel performance on both SERS detection and sample pretreatment. A biosensor for Mucin-1 is developed by equipping the Au NPs with aptamer.

Dual-Functional Carbon Dots Pattern on Paper Chips for Fe and Ferritin Analysis in Whole Blood.

Though microfluidic paper analytical devices (μPADs) have attracted paramounting attentions in recent years as promising devices for low cost point-of-care tests, their real applications for blood analysis are still challenged by integrating sample preparation with different detection modes on a same μPAD. Herein, we developed a novel μPAD, which well coupled automatic serum extraction with reliable dual mode iron health tests: fluorescent analysis for Fe and colorimetric ELISA for ferritin. All these functions are made available by in situ carbon dots (CDs) and AuNPs sequential patterning techniques.

A microfluidic cigarette smoke collecting platform for simultaneous sample extraction and multiplex analysis.

In this work, we report a novel microfluidic gas collecting platform aiming at simultaneous sample extraction and multiplex mass spectrometry (MS) analysis. An alveolar-mimicking elastic polydimethylsiloxane (PDMS) structures was designed to move dynamically driven by external pressure. The movement was well tuned both by its amplitude and rhythm following the natural process of human respiration.

Versatile microfluidic droplets array for bioanalysis.

We propose a novel method to obtain versatile droplets arrays on a regional hydrophilic chip that is fabricated by PDMS soft lithography and regional plasma treatment. It enables rapid liquid dispensation and droplets array formation just making the chip surface in contact with solution. By combining this chip with a special Christmas Tree structure, the droplets array with concentrations in gradient is generated.

Liquid gradient in two-dimensional matrix for high throughput screening.

Based on the ingenious combination of two different gradient generation mechanisms, this work reports a novel approach for a high throughput linear liquid gradient in a two-dimensional (2D) matrix. Specifically, a typical Christmas Tree structure with two inlets was designed as the first mixture gradient generator, upon which the second diffusion gradient generator was coupled to produce the desired concentration series on the basis of the distance difference. Rather than a simple 1D line, the integration of the two generators would result in an innovative 2D matrix of reservoirs, which was then characterized both theoretically and experimentally.

A novel microfluidic platform with stable concentration gradient for on chip cell culture and screening assays.

In this work a novel microfluidic platform for cell culture and assay is developed. On the chip a static cell culture region is coupled with dynamic fluidic nutrition supply structures. The cell culture unit has a sandwich structure with liquid channels on the top, the cell culture reservoir in the middle and gas channels on the bottom.

On chip steady liquid-gas phase separation for flexible generation of dissolved gas concentration gradient.

In this study, steady liquid-gas phase separation is realized by applying a hydrophobic small microchannel array (SMA) to bridge two large microchannels, one for liquid phase and one for gas phase. In this structure, a capillary pressure difference between that in the SMA and the larger channel results in a steady liquid-gas interface. The generated liquid-gas interface allows for fast gas dissolving speed.