A QTL of eggplant shapes the rhizosphere bacterial community, co-responsible for resistance to bacterial wilt.

Resistant crop cultivars can recruit beneficial rhizobacteria to resist disease. However, whether this recruitment is regulated by quantitative trait loci (QTL) is unclear. The role of QTL in recruiting specific bacteria against bacterial wilt (BW) is an important question of practical significance to disease management.

Comparative analysis of the medicinal and nutritional components of different varieties of and .

and are important medicinal plants with unique chemical compositions that are widely used in traditional Chinese medicine. To compare the nutritional and medicinal profiles of these two species, we analyzed the flavonoid, dietary fiber, total starch, and crude protein contents of one and three varieties using ultra-performance liquid chromatography-tandem mass spectrometry, enzyme weight, acid hydrolysis, and Kjeldahl methods. Furthermore, we used principal component analysis and hierarchical clustering heatmap analysis to separate the data obtained from the and samples.

A chromosome-level reference genome of the wax gourd (Benincasa hispida).

The wax gourd (Benincasa hispida), the only species in the genus Benincasa, is an important crop native to Asia that has been widely planted for multi-purpose uses. The first wax gourd draft genome was published three years ago, but it was incomplete and highly-fragmented due to data and technical limitations. Herein, we report a new chromosome-level genome assembly and annotation of B.

Genome and transcriptome-based characterization of high energy carbon-ion beam irradiation induced delayed flower senescence mutant in Lotus japonicus.

Background: Flower longevity is closely related to pollen dispersal and reproductive success in all plants, as well as the commercial value of ornamental plants. Mutants that display variation in flower longevity are useful tools for understanding the mechanisms underlying this trait. Heavy-ion beam irradiation has great potential to improve flower shapes and colors; however, few studies are available on the mutation of flower senescence in leguminous plants.

Molybdenum disulfide nanoparticles concurrently stimulated biomass and β-carotene accumulation in Dunaliella salina.

Molybdenum disulfide nanoparticles (MoS NPs) hold tremendous properties in wide domain of applications. In this study, the impact of MoS NPs was investigated on algal physiological and metabolic properties and a two-stage strategy was acquired to enhance the commercial potential of Dunaliella salina. With 50 µg/L of MoS NPs exposure, cellular growth and biomass production were promoted by 1.

Physiological and molecular responses in halotolerant Dunaliella salina exposed to molybdenum disulfide nanoparticles.

Molybdenum disulfide nanoparticles (MoS NPs) has emerged as the promising nanomaterial with a wide array of applications in the biomedical, industrial and environmental field. However, the potential effect of MoS NPs on marine organisms has yet to be reported. In this study, the effect of MoS NPs on the physiological index, subcellular morphology, transcriptomic profiles of the marine microalgae Dunaliella salina was investigated for the first time.

Genetic polymorphisms in mutagenesis progeny of irradiated by carbon-ion beams and γ-rays irradiations.

Heavy-ion beams and γ-rays are popular physical mutagenesis to generate mutations in higher plants. It has been found that they show different mutation frequencies and spectrums of phenotype induction, however, the characteristics of heavy-ion beams on genetic polymorphism have not been clarified by comparing with γ-rays. In the present study, seeds of were exposed to carbon-ion beams (with linear energy transfer (LET) of 50 keV/μm) and γ-rays (with average LET of 0.

Ethanol induced jasmonate pathway promotes astaxanthin hyperaccumulation in Haematococcus pluvialis.

Haematococcus pluvialis is a main biological resource for the antioxidant astaxanthin production, however, potential modulators and molecular mechanisms underpinning astaxanthin accumulation remain largely obscured. We discovered that provision of ethanol (0.4%) significantly triggered the cellular astaxanthin content up to 3.

Adaptive evolution of microalgal strains empowered by fulvic acid for enhanced polyunsaturated fatty acid production.

Microalgae have emerged as the potential source for value-added products such as polyunsaturated fatty acids (PUFAs). Metabolic engineering of multiple metabolic pathways has promoted eicosapentaenoic acid (EPA) production in microalgae, however, further improvement is warranted owing to the burgeoning demand. Here we improved the microalgal strains by adaptive evolution under hyposalinity treatment, which showed that 70% salinity potentiated the algae to enhance PUFAs.

Strategies for identification of mutations induced by carbon-ion beam irradiation in Arabidopsis thaliana by whole genome re-sequencing.

Heavy-ion beam irradiation is a powerful physical mutagen that has been used to create numerous mutant materials in plants. These materials are an essential resource for functional genomics research in the post-genome era. The advent of Next-Generation Sequencing (NGS) technology has promoted the study of functional genomics and molecular breeding.

Identification of Substitutions and Small Insertion-Deletions Induced by Carbon-Ion Beam Irradiation in .

Heavy-ion beam irradiation is one of the principal methods used to create mutants in plants. Research on mutagenic effects and molecular mechanisms of radiation is an important subject that is multi-disciplinary. Here, we re-sequenced 11 mutagenesis progeny (M3) lines derived from carbon-ion beam (CIB) irradiation, and subsequently focused on substitutions and small insertion-deletion (INDELs).

Mutagenic effects of carbon-ion irradiation on dry Arabidopsis thaliana seeds.

To investigate the mutagenic effects of carbon ions on Arabidopsis thaliana (ecotype Columbia) and to isolate useful genes in plant development, dry seeds were exposed to 43MeV/u carbon ions at doses of 0, 100, 200, 300, 400, 500 and 600Gy. The survival rate, primary root length, and hypocotyl length of M1 plants were analyzed, and 200Gy was selected as the dose for the large-scale experiment. A total of 1363 lines of plants from 28,062 M2 populations displayed alterations in the leaf, stem, flower, or life cycle, with abnormal leaves and a premature life cycle as the main phenotypic variations.