A Forward Genetic Approach to Mapping a -Element Second Site Mutation Identifies as a Novel Tumor Suppressor in .

The use of transposons to create mutations has been the cornerstone of genetics in the past few decades. Second-site mutations caused by transpositions are often devoid of transposons and thereby affect subsequent analyses. In a -element mutagenesis screen, a second site mutation was identified on chromosome 3, wherein the homozygous mutants exhibit classic hallmarks of tumor suppressor mutants, including brain tumor and lethality; hence the mutant line was initially named as [].