Publications by authors named "Shannon Ceballos"

Article Synopsis
  • The yeast Komagataella phaffii is favored by biotech startups for producing recombinant proteins due to its safety record and effective production processes, but recent findings reveal a significant polysaccharide accumulation during fermentation that complicates product purity and adds processing costs.
  • The study focused on using K. phaffii strain YB-4290 to produce lactoferrin, uncovering that a considerable amount of carbohydrate (mainly composed of mannose) co-purifies with the protein, indicating challenges in purification methods.
  • Further investigations into carbohydrate sources showed that commonly used strains produce baseline exopolysaccharides, with higher levels occurring under recombinant protein expression, which may inform strategies for improving efficiency in protein production with K. ph
View Article and Find Full Text PDF

Stalled replication forks can be processed by several distinct mechanisms collectively called post-replication repair which includes homologous recombination, fork regression, and translesion DNA synthesis. However, the regulation of the usage between these pathways is not fully understood. The Rad51 protein plays a pivotal role in maintaining genomic stability through its roles in HR and in protecting stalled replication forks from degradation.

View Article and Find Full Text PDF

Background: The increasing production of almonds worldwide has resulted in the significant generation of byproduct streams that require end uses. One potential use for byproducts is for cultivation of additional food sources including insects. Studies were performed to determine if black soldier fly larvae (Hermetia illucens L.

View Article and Find Full Text PDF

Ionic liquid (IL) pretreatment is a promising approach for the conversion of lignocellulose to biofuels. The toxicity of residual IL, however, negatively impacts the performance of industrial enzymes and microorganisms in hydrolysis and fermentation. In this study, a thermophilic microbial community was cultured on switchgrass amended with various levels of the ionic liquid 1-ethyl-3-methylimidazolium acetate.

View Article and Find Full Text PDF

The aims of this study were to identify thermophilic microbial communities that degrade green waste in the presence of the ionic liquids (IL) tetrabutylphosphonium chloride and tributylethylphosphonium diethylphosphate and examine preservation methods for IL-tolerant communities. High-solids incubations with stepwise increases in IL concentration were conducted to enrich for thermophilic IL-tolerant communities that decomposed green waste. 16S rRNA sequencing of enriched communities revealed microorganisms capable of tolerating high levels of IL.

View Article and Find Full Text PDF

Homologous recombination is a central pathway to maintain genomic stability and is involved in the repair of DNA damage and replication fork support, as well as accurate chromosome segregation during meiosis. Rad54 is a dsDNA-dependent ATPase of the Snf2/Swi2 family of SF2 helicases, although Rad54 lacks classical helicase activity and cannot carry out the strand displacement reactions typical for DNA helicases. Rad54 is a potent and processive motor protein that translocates on dsDNA, potentially executing several functions in recombinational DNA repair.

View Article and Find Full Text PDF