Effects of Euphorbia esula L. (leafy spurge) on cattle and sheep in vitro fermentation and gas production.

Background: Euphorbia esula L. (leafy spurge) is indigenous to Eurasia and has been known to cause grazing aversion in ruminant species. As a result, E.

Determination of methylglyoxal in ruminal fluid by high-performance liquid chromatography using fluorometric detection.

There is no reported method for the quantification of methylglyoxal in ruminal fluid. The method reported here is based on the conversion of methylglyoxal to 6-methylpterin, followed by quantification of the resulting pteridinic compound by fluormetric detection using liquid chromatography. Ruminal fluid was collected and preserved with 1 M HCl at -20 degrees C.