Publications by authors named "Shanna A Santos"
Standardization and Evaluation of the LAMP Technique for the Diagnosis of Canine Visceral Leishmaniasis in Conjunctival Swab Samples Using DNA Extracted by a Silica Column and Boiling.
Trop Med Infect Dis
November 2024
The diagnosis of canine visceral leishmaniasis (CVL) presents a challenge due to a variety of non-specific clinical signs. The available tests have low sensitivity. This study aimed to standardize and evaluate the loop-mediated isothermal amplification technique with K26 target (K26-LAMP) for diagnosis of CVL in conjunctival swab (CS) DNA samples extracted through a silica column commercial kit (SW-kit) and boiling (SW-DB) and to compare sensitivity with conventional PCR (kDNA-cPCR) and quantitative real-time PCR (18S-qPCR).
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- * Out of 39 pets observed, about 31% of dogs and 40% of cats showed signs of infection or had antibodies for the virus, with some test results persisting several weeks after the initial human diagnosis.
- * The study suggests that pet owners with COVID-19 should minimize close contact with their pets to reduce the risk of transmission, especially since some infected animals exhibited mild illness symptoms.
Frequency of co-seropositivities for certain pathogens and their relationship with clinical and histopathological changes and parasite load in dogs infected with Leishmania infantum.
PLoS One
September 2021
Article Synopsis
- The study investigates how co-infections with other pathogens affect dogs infected with Leishmania infantum, the cause of canine leishmaniosis.
- Out of 66 dogs seropositive for L. infantum, 76% were also co-seropositive for other pathogens, particularly Toxoplasma gondii and Ehrlichia spp.
- Although clinical signs were present in a majority of co-seropositive dogs, the overall disease severity and L. infantum load remained similar between monoinfected and co-infected dogs, although inflammatory responses were increased in co-seropositive cases.
Frequency, active infection and load of Leishmania infantum and associated histological alterations in the genital tract of male and female dogs.
PLoS One
October 2020
Visceral leishmaniasis caused by the protozoan Leishmania infantum is a zoonosis. The domestic dog is the primary reservoir in urban areas. This study aimed to evaluate the frequency, active infection and load of L.
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